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GB/T 35329-2017: Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 35329-2017 | English | 279 |
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Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell
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GB/T 35329-2017
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Basic data | Standard ID | GB/T 35329-2017 (GB/T35329-2017) | | Description (Translated English) | Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,152 | | Date of Issue | 2017-12-29 | | Date of Implementation | 2018-07-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 35329-2017: Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell
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Detection and identification of Phytophthora medicaginis EMHans.et DPMaxwell
ICS 65.020.01
B16
National Standards of People's Republic of China
Quarantine and identification methods for root rot pathogens
Published on.2017-12-29
2018-07-01 Implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing agency of this document does not assume responsibility for identifying these patents.
This standard is proposed and managed by the National Commission for Standardization of Plant Quarantine (SAC/TC271).
This standard was drafted by the People's Republic of China Tianjin Entry-Exit Inspection and Quarantine Bureau and the Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Zhang Yujun, Du Hongzhong, Guo Jingze, He Yan, Liu Peng, Liu Yueting, Wu Pinshan.
Quarantine and identification methods for root rot pathogens
1 Scope
This standard specifies the quarantine and identification methods for the phytophthora infestans.
This standard applies to plants, plant products, and entrained soils and mediums that target the host of Phytophthora capsici (see Appendix A).
Quarantine and identification of the phytophthora root rot pathogen.
2 Phytophthora root rot pathogen basic information
Scientific name. PhytophthoramedicaginisE.M.Hans.etD.P.Maxwel.
Taxonomic status. Chromista, Oomycota, Peronosporea, Peronospora-
Les), Peronosporaceae, Phytophthora.
Transmission route. soil, flowing water, cultivation medium, seedlings, root tissues can all be transmitted and transmitted, and dry grasses traded and transported also have long distances.
The risk of transmission.
See Appendix A for additional information on P. grisea root rot.
3 Methodology
Pathological symptoms of host plants, morphological characteristics of pathogenic bacteria, and sequence characteristics of pathogenic bacteria genome target fragments
collection.
4 Instruments and apparatus
4.1 Instruments
Clean bench, light incubator, artificial climate box, biological microscope, electronic balance (sensibility 0.01g), autoclave, blood count
, Constant temperature water bath, High-speed refrigerated centrifuge, Ice maker, Spectrophotometer, PCR amplification instrument, Electrophoresis apparatus, Gel imaging system, Fluorescent PCR
The instrument.
4.2 Appliances
Beaker, flask, graduated cylinder, petri dish, alcohol lamp, tweezers, scissors, scalpel, inoculation needle, slide, coverslip, plastic mortar, pipette
Device, pipette tips, centrifuge tubes.
5 Reagents and culture media
5.1 Reagent materials
Unless otherwise specified, all reagents were of analytical grade. Pimamarycin (C33H47NO13), Ampicillin Sodium Salt (C16H18N3O4SNa),
Rifampicin sodium salt (C43H58N4O12), pentachloronitrobenzene (C6C15NO2), calcium carbonate (CaCO3), sodium hypochlorite (NaOCl).
Tris(hydroxymethyl)aminomethane (C4H11NO3), Sodium Chloride (NaCl2), Hydrochloric acid (HCl), Tetrasodium ethylenediaminetetraacetate (C10H4N2O8Na2),
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