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GB/T 35098-2018: Microbeam analysis -- Transmission electron microscopy -- Morphological identification method of plant viruses by transmission electron microscopy
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| GB/T 35098-2018 | English | 379 |
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Microbeam analysis -- Transmission electron microscopy -- Morphological identification method of plant viruses by transmission electron microscopy
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GB/T 35098-2018
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Basic data | Standard ID | GB/T 35098-2018 (GB/T35098-2018) | | Description (Translated English) | Microbeam analysis -- Transmission electron microscopy -- Morphological identification method of plant viruses by transmission electron microscopy | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G04 | | Classification of International Standard | 71.040.40 | | Word Count Estimation | 19,162 | | Date of Issue | 2018-05-14 | | Date of Implementation | 2019-04-01 | | Regulation (derived from) | National Standards Announcement No. 6 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35098-2018: Microbeam analysis -- Transmission electron microscopy -- Morphological identification method of plant viruses by transmission electron microscopy
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(Microbeam analysis Transmission electron microscopy Identification of plant virus morphology by transmission electron microscopy)
ICS 71.040.40
G04
National Standards of People's Republic of China
Microbeam analysis transmission electron microscopy plant virus
Morphological transmission electron microscopy identification method
Published on.2018-05-14
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Content
Foreword I
Introduction II
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Basic Principles 2
5 Instruments, reagents and materials, environmental conditions 2
6 sample 3
7 Transmission electron microscopy identification step 5
8 Quality requirements for morphological identification of plant viruses 6
9 Identification Report Release 6
10 Decontamination treatment 6
Appendix A (informative) Formulations for commonly used reagents 7
Appendix B (informative) Morphological and cytopathological features of various families and genera of plant viruses 10
Reference 15
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Microbeam Analysis Standardization Technical Committee (SAC/TC38).
This standard was drafted. Zhejiang University, Yunnan Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences.
The main drafters of this standard. Hong Jian, Zhang Zhongkai, Xie Li, Wu Jianxiang, Qian Yajuan, Zhou Xueping.
Introduction
Plant viruses are an important class of pathogens that infect angiosperms, gymnosperms and ferns, causing crops worldwide.
Diseases of fruits, fruit trees, flowers, pastures, and medicinal plants cause a decline in yield and quality, which seriously affects human production and life..2016 International
The plant virus announced by the International Committee for Classification (Taxonomy of Viruses, ICTV) involves 4
Head, 26 families, 5 subfamilies, 118 genera, a total of 1323 species. Virology morphology is an important basis for virus identification and classification, based on viral particles
The shape, size, presence or absence of the capsule, the symmetry of the capsid, the cytopathology of the virus in the host, and the genomic sequence
Equivalent molecular biology evidence can be used to diagnose and identify viruses to families, genera, and species. Since the individual of the plant virus is as small as several tens of nanometers, it can be transmitted by electrons.
The microscope can observe its morphology. In order to standardize the technical methods of plant virus morphology detection, correctly guide the diagnosis of plant virus pathogens
And identification work, it is necessary to develop a national standard for morphological identification methods of plant virus transmission electron microscopy.
Microbeam analysis transmission electron microscopy plant virus
Morphological transmission electron microscopy identification method
1 Scope
This standard specifies the steps, quality requirements and identification reports for morphological observation and identification of plant viruses using transmission electron microscopy.
The announcement of the announcement.
This standard applies to the observation and identification of plant virus morphology by various types of transmission electron microscopy.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products
SN/T 2964 Plant Virus Testing Practice
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Virus virus
A pathogen consisting of a nucleic acid and a protein coat and having an invasive activity and capable of self-replication in a cell.
3.2
Virion virion
A mature or structurally intact, infectious virus individual.
3.3
Capsid capsid
A symmetrical protein envelope consisting of a collection of protein subunits and surrounding the nucleic acid genome.
3.4
Nucleocapsid nucleocapsid
A combination of a protein capsid surrounding a viral nucleic acid and a nucleic acid.
3.5
Inclusion body
An abnormal structure composed of viruses or cellular components produced in a host cell after infection by a virus.
Note. The inclusion bodies show different morphological features under optical or TEM.
3.6
Antigen antigen
A substance that stimulates the body of an animal to produce antibodies and sensitize lymphocytes and to react specifically with it.
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