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GB/T 34918-2017: Determination of 7 sexual hormones in cosmetics -- Ultra high performance liquid chromatography-tandem mass spectrometry
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 34918-2017 | English | 219 |
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Determination of 7 sexual hormones in cosmetics -- Ultra high performance liquid chromatography-tandem mass spectrometry
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GB/T 34918-2017
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Basic data | Standard ID | GB/T 34918-2017 (GB/T34918-2017) | | Description (Translated English) | Determination of 7 sexual hormones in cosmetics -- Ultra high performance liquid chromatography-tandem mass spectrometry | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 11,125 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34918-2017: Determination of 7 sexual hormones in cosmetics -- Ultra high performance liquid chromatography-tandem mass spectrometry
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Determination of 7 sexual hormones in cosmetics-Ultra high performance liquid chromatography-tandem mass spectrometry
ICS 71.100.70
Y42
National Standards of People's Republic of China
Determination of seven sex hormones in cosmetics
Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Quality Supervision Key Products Testing Methods Standardization Technical Committee (SAC/TC374) and focal point.
This standard was drafted unit. Shenzhen Institute of Measurement and Quality Inspection, the quality of check Warner (Beijing) Co., Ltd. Technology Center, the Federation seized
(Beijing) Co., Ltd. Quality Inspection Institute, the National Commodity Quality Supervision and Inspection Center, Shenzhen City, large capacity biotechnology Co., Ltd.,
Shenzhen Jin Yin Biotechnology Co., Ltd., Zhejiang Yan Zhuang Cosmetics Co., Ltd., Infinitus (China) Co., Ltd., Perfect (China) Co., Ltd.
Division, Shanghai affordable herbal Cosmetics Co., Ltd., Guangdong Danzi Group Co., Ltd., Guangzhou City, the United States and Cosmetics Co., Ltd., Guangdong Bawei
Biotechnology Co., Ltd., NICE Group Co., Ltd., Yiwu City Products (Commodity) Quality Supervision, Inspection and Research Institute.
The main drafters of this standard. Zhao Yan, Lin Chi-hui, lucky na, Feng shore red, Chen Qian, Xu Honghui, Yang Ping Shun, Ge Feng, Sun Hongmei,
Yao Songjun, Li Xiaomin, Yuan Liwen, Lu Zhi, Mao Yongjin, Zhang Jian, Liu Ruixue, Duan Jianjun, Wei Shunwen.
Determination of seven sex hormones in cosmetics
Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry
Warning --- personnel using this standard should have formal laboratory work experience. The use of this standard may involve some in danger
Dangerous materials, equipment and operation, this standard does not indicate all possible security issues. It is the user's responsibility to take appropriate safety and health measures
Shi, and ensure compliance with the conditions of the relevant state laws and regulations.
1 Scope
This standard specifies the ultra-high performance liquid chromatography-tandem mass spectrometry water, milk, cream, mask, powder and other cosmetics in seven kinds of irritation
Principles of estrogen (estriol, estradiol, estrone, diethylstilbestrol, testosterone, methyltestosterone, progesterone), reagents, equipment, sample handling, assays,
Experimental data processing and experimental reports.
This standard applies to water, milk, cream, mask, powder and other cosmetics in the detection of seven sex hormones.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 principle
With methanol, acetonitrile (1 1, volume ratio) mixed solvent swirling cosmetics sex hormones, centrifugal filtration, adding sodium hydroxide solution
Liquid saponification reaction, adjust the pH to 6.5 ~ 7.5, purified by solid phase extraction cartridge, determined by reverse-phase ultra performance liquid chromatography - tandem mass spectrometry,
Internal standard method.
4 Reagents or materials
Unless otherwise specified, only analytical reagents are used.
4.1 GB/T 6682 provisions of a water.
4.2 methanol. Chromatography pure.
4.3 acetonitrile. Chromatographic purity.
4.4 Ammonium acetate.
4.5 Sodium hydroxide.
4.6 concentrated hydrochloric acid.
4.7 Methanol Acetonitrile (1 1, volume ratio), respectively, the amount of 500mL methanol (4.2) and 500mL acetonitrile (4.3) after mixing well.
4.8 Ammonium acetate solution (0.5 mmol/L) Weigh 38.5 mg of ammonium acetate (4.4) and dissolve to 1 L with water (4.1).
4.9 Sodium hydroxide solution (20g/L) Weigh 20.0g sodium hydroxide (4.5) and dissolve to 1L with water (4.1).
4.10 Hydrochloric acid solution (10mol/L). Take 833mL of concentrated hydrochloric acid (4.6) and dissolve to 1L with water (4.1).
4.11 Hydrochloric acid solution (1mol/L). Measure 100mL of 10mol/L hydrochloric acid solution (4.10) and dissolve to 1L with water (4.1).
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