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GB/T 34799-2017: Determination of the activity of chitinase
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| GB/T 34799-2017 | English | 119 |
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Determination of the activity of chitinase
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GB/T 34799-2017
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Basic data | Standard ID | GB/T 34799-2017 (GB/T34799-2017) | | Description (Translated English) | Determination of the activity of chitinase | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G66 | | Classification of International Standard | 07.080 | | Word Count Estimation | 6,623 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34799-2017: Determination of the activity of chitinase---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of chitinase
ICS 07.080
G66
National Standards of People's Republic of China
Chitinase activity test method
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized.
This standard was drafted by Shenzhen Institute of Measurement and Quality Inspection, China Institute of Testing Technology, Sichuan Province Health and Family Planning Supervision
Law Enforcement Corps, Shenzhen Polytechnic.
The main drafters of this standard.Li Bifang, Lai Xin Tian, Lu Jie, Zhang Shiwei, Zhou Lihua, Liu Dong, Lin Lin, Tang Dong, Wang Shifeng, Chen Xuejian, Liu Bin,
Yang Guowu.
Chitinase activity test method
1 Scope
This standard specifies Serratia marcescens, Streptomyces griseus and green wood
Detection of Chitinase Activity by Trichodermaviride Fermentation.
This standard applies to biochemical reagents, determination of chitinase activity in industrial products.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Chitinase activity unit chitinaseactivityunit
At 37 ° C, pH 6.0, β-N-acetylglucosaminidase-assisted two-step reaction catalyzed the formation of 1 mg N-B per minute
The amount of chitinase in acylglucose is one unit of activity, in units of U.
4 principle
Chitinase rapidly catalyzes the hydrolysis of chitin to N-acetylglucose or disaccharides and oligosaccharides composed of N-acetylglucose, disaccharides and
Oligosaccharides continue to be hydrolyzed with N-acetylglucosaminidase to N-acetylglucose, which is detectable by absorbance,
Calculated N-acetyl glucose production measured enzyme activity.
5 equipment and appliances
5.1 pH meter.
5.2 electronic balance. Feel 0.0001g.
5.3 Spectrophotometer. Absorbance value accurate to 0.001.
5.4 1cm quartz cuvette.
5.5 constant temperature shaking shaker temperature control accuracy of ± 0.5 ℃.
6 Reagents
The reagents used in this standard are of analytical grade, water is in line with GB/T 6682 provisions of the two water.
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