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GB/T 34059-2017 English PDF

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GB/T 34059-2017: Nanotechnology -- Nano-biological effects of nanomaterials -- NMR-based metabolomics
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Basic data

Standard ID GB/T 34059-2017 (GB/T34059-2017)
Description (Translated English) Nanotechnology -- Nano-biological effects of nanomaterials -- NMR-based metabolomics
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard N04
Classification of International Standard 71.040.50
Word Count Estimation 18,158
Date of Issue 2017-07-31
Date of Implementation 2018-02-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34059-2017: Nanotechnology -- Nano-biological effects of nanomaterials -- NMR-based metabolomics


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Nanotechnology - Nanobiological effects of nanomaterials - NMR-based metabolomics ICS 71.040.50 N04 National Standards of People's Republic of China Nanotechnology Nanobiotic Effects Metabonomics Methods Nuclear magnetic resonance spectroscopy 2017-07-31 Posted 2018-02-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Foreword Ⅲ Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Abbreviations 1 Principle 5 6 instruments 1 7 Test Sample 2 8 test step 2 9 signal attribution 2 10 signal pretreatment and multivariate statistical analysis 2 11 test analysis results 3 Appendix A (Normative) Acronyms 4 Appendix B (informative) Gold nanorods on cytotoxicity Example 5 Appendix C (Informative) Nano-biological effects of NMR test report format 11 Reference 12

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the Chinese Academy of Sciences. This standard by the National Institute of Technology Standardization Technical Committee (SAC/TC279) centralized. This standard drafting unit. Chinese Academy of Sciences Wuhan Institute of Physics and Mathematics, National Nanoscience and Technology Center. The main drafters of this standard. Zhang Limin, Wang Yulan, Tang Huiru, Wu Xiaochun.

Introduction

Nanomaterials can enter the organism through many ways, such as breathing, ingesting, direct or indirect skin contact, etc., Sub-levels affect the organism, causing metabolic changes in the organism. Therefore, it can be evaluated by the changes of metabolome of organism caused by the exposure of nanomaterials Biological effect of nanomaterials. Metabolomics is based on group analysis of indicators, high-throughput detection and multivariate statistics Analysis as a means to mathematical models and systems integration as the goal of the discipline, is a branch of system biology, has been widely used in swollen Tumor, gastrointestinal diseases, metabolic diseases and toxicology and other fields. At present metabolomics test methods are mainly chromatography-mass spectrometry and Nuclear magnetic resonance spectroscopy, the standard developed based on nuclear magnetic resonance spectroscopy to detect and used to evaluate the biological effects of nanomaterials metabonomics analysis method. Nanotechnology Nanobiotic Effects Metabonomics Methods Nuclear magnetic resonance spectroscopy

1 Scope

This standard specifies the nuclear magnetic resonance detection method of nano-material exposure caused by changes in the metabolism of organisms. This standard applies to biological effects of nano-materials evaluation.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 13966 analytical instruments terminology GB/T 19619 nanomaterials terminology

3 Terms and definitions

GB/T 13966 and GB/T 19619 and defined by the following terms and definitions apply to this document. 3.1 Metabolomics metabonomics; metabolomics Metabolites in vivo analysis of the group, and the establishment of metabolites and physiological and pathological changes in the correspondence between the omics method [1]. 3.2 Nanomaterials exposed nanomaterialsexposure Nanomaterials are in full contact with the population or with experimental cells and animals.

4 Abbreviations

See Appendix A.

5 principle

Proton NMR was used to detect biological samples exposed to nanomaterials, and multivariate statistical analysis of metabolomics Changes in content, thus assess the biological effects of nano-materials.

6 instruments

6.1 High-resolution nuclear magnetic resonance spectrometer NMR spectrometer with proton resonance frequency above 500MHz, see Appendix B for parameter optimization.

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