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GB/T 33807-2017 English PDF

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GB/T 33807-2017: Detection of genetically modified components in maize -- Gene chip method
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PDF similar to GB/T 33807-2017


Standard similar to GB/T 33807-2017

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Basic data

Standard ID GB/T 33807-2017 (GB/T33807-2017)
Description (Translated English) Detection of genetically modified components in maize -- Gene chip method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B04
Classification of International Standard 67.050
Word Count Estimation 14,170
Date of Issue 2017-05-31
Date of Implementation 2017-12-01
Quoted Standard GB/T 6682; GB/T 19495.1; GB/T 19495.2; GB/T 19495.3; GB/T 19495.6; GB/T 19495.7
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the detection methods for transgenic components in maize and maize processed products. This standard is suitable for qualitative detection of transgenic elements of transgenic EPSPS gene, PAT gene, BAR gene herbicide tolerant maize and transgenic Bt gene (Cry1A105, Cry1Ab), and also for the qualitative detection of transgenic components in maize processed products. The minimum limit for detection of transgenic components is 0.1%.

GB/T 33807-2017: Detection of genetically modified components in maize -- Gene chip method

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Detection of genetically modified components in maize - Gene chip method ICS 67.050 B04 National Standards of People's Republic of China Determination of Transgenic Genes in Maize Gene chip method Detection ofgeneticalymodifiedcomponentsinmaize- Genechipmethod 2017-05-31 released 2017-12-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and centralized by the National Standardization Technical Committee for Biochip (SAC/TC421). The main drafting unit of this standard. Sichuan Huahan three biological technology Co., Ltd., the National Agricultural Standardization Monitoring and Research Center (Black Dragon Jiang), Guangdong Product Quality Supervision and Inspection Research Institute, Heilongjiang Province, grain and oil health inspection and monitoring station. The main drafters of this standard. Huang Guangping, Peng Liping, She Shenyun, Zhang Jinlong, Wang Zhiqiang, Wang Yongqiang, Kong Luyi, Wei Minwei, Zhang Chunhong, Yin Zhijian, Wang Haikuan. Determination of Transgenic Genes in Maize Gene chip method

1 Scope

This standard specifies the detection methods for transgenic components in maize and maize processed products. This standard is applicable to transgenic EPSPS gene, PAT gene, BAR gene herbicide resistant maize and transgenic Bt gene (Cry1A105, Cry1Ab) insect-resistant maize transgenic components of qualitative testing, but also for corn processing products in the qualitative detection of genetically modified ingredients, the standard The minimum detection limit for the defined transgenic component is 0.1%.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 19495.1 General requirements and definitions for the detection of genetically modified products GB/T 19495.2 Technical requirements for laboratory testing of transgenic products Method for extraction and purification of nucleic acid for detection of transgenic products GB/T 19495 GB/T 19495.6 Detection of genetically modified products by gene chip detection Sampling and sample preparation methods for transgenic product testing GB/T 3 terms, definitions and abbreviations 3.1 Terms and definitions GB/T 19495.1, GB/T 19495.6 Definitions of terms and definitions apply to this document. 3.2 abbreviations The following abbreviations apply to this document. BAR. derived from Bacillus Bacillusamyloliquefaciens glufosinate acetyltransferase gene (phosphinothricinacetyl- transferasegene bp. base pair (basepair) CaMV35S-P. 35S promoter from the cauliflower mosaic virus (Promoterfromcauliflowermosaicvirus) CaMV35S-T. 35S terminator from cauliflower mosaic virus (Terminationsequencefromcauliflowermo- saicvirus Cry1Ab. Bacillus thuringiensis Insecticidal protein Cry1Ab gene (BacillusthuringiensisInsecticidaltoxin Cry1Abgene) Cry1A105. Bacillus thuringiensis Insecticidal Protein Cry1A105 Gene (BacillusthuringiensisInsecticidaltoxin Cry1A105gene)

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