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GB/T 32016-2015 English PDF

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GB/T 32016-2015: Silk -- Determination of amino acids
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PDF similar to GB/T 32016-2015


Standard similar to GB/T 32016-2015

FZ/T 50015   GB/T 32015   GB/T 32014   

Basic data

Standard ID GB/T 32016-2015 (GB/T32016-2015)
Description (Translated English) Silk -- Determination of amino acids
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard W40
Classification of International Standard 59.080.01
Word Count Estimation 10,169
Date of Issue 2015-09-11
Date of Implementation 2016-04-01
Quoted Standard GB/T 6529; GB/T 6682; GB/T 8170
Regulation (derived from) National Standard Announcement 2015 No.25
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This Standard specifies the method for Determination of silk amino acid composition and content. This Standard applies to the determination of the silk in eighteen amino acids components. Aspartic acid (Asp), threonine (Thr), serine (Ser), glutamate (Glu), glycine (Gly), alanine (Ala), valine (Val), isoleucine (Ile), leucine (Leu), tyrosine (Tyr), phenylalanine (Phe), lysine (Lys), histidine (His), arginine (Arg), proline (Pro) was measured using acid hydrolysis, cystine (Cys) and methionine (Met) if not accurately measured by acid hydrolysis, oxidative hydrolysis assay should be used, tryptophan (Trp) was measured using alkaline hydrolysis. The minimum detection limits were 10 pmol.

GB/T 32016-2015: Silk -- Determination of amino acids

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(Determination of Silk Amino Acids) ICS 59.080.01 W40 National Standards of People's Republic of China Determination of silk amino acids Issued on. 2015-09-11 2016-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by China Textile Industry Association. This standard by the National Standardization Technical Committee silk (SAC/TC401) centralized. Drafting of this standard. Zhejiang Silk Technology Co., Ltd., Guangzhou Academy of fiber product testing, Xinyuan Silk Group shares have Ltd., Suzhou University, Jiangmen Exit Inspection and Quarantine, Hangzhou Quality and Technical Supervision and Inspection Institute, Guangdong CIQ, Zhejiang Provincial Inspection and Quarantine Institute of Science and Technology. The main drafters of this standard. Wu Dongping, Li Tian, \u200b\u200bWushu Huan, Xuhao Nan Sun Road right, Paul Sarkozy left, right Cham, Liu Yu, Xiao Hailong, Gu tiger, Li Chun, Zhou Ying, Wu Gang. Determination of silk amino acids

1 Scope

This standard specifies the method for Determination of silk amino acid composition and content. This standard applies to the determination of the silk in eighteen amino acids components. Aspartic acid (Asp), threonine (Thr), serine (Ser), Valley Acid (Glu), glycine (Gly), alanine (Ala), valine (Va1), isoleucine (Ile), leucine (Leu), tyrosine (Tyr), benzene Phenylalanine (Phe), lysine (Lys), histidine (His), arginine (Arg), proline (Pro) was measured using acid hydrolysis, cystine (Cys) and methionine (Met) if the acid hydrolysis can not be accurately determined by measuring the oxidation hydrolysis method should be used, tryptophan (Trp) using alkaline hydrolysis Determination. The minimum detection limits were 10pmol.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 6529 Textiles Standard conditioning and testing atmosphere Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 8170 repair value expressed about the rules and limit values \u200b\u200band judgment

3 PRINCIPLE OF THE METHOD

Acid, alkaline hydrolysis. hydrolyzed silk protein amino acids occur in high-temperature acidic or alkaline solution, hydrolysis into usable ion exchange chromatography Separation and determination method. Hydrolysis of. silk protein sulfur amino acids (cysteine, methionine), used by the oxidation of formic acid and aqueous hydrochloric acid Solution Generation cysteic acid and methionine alum, chromatography separation and determination of these two products are available ion exchange.

4 Reagents and materials

4.1 Requirements Concentrated hydrochloric acid (12mol/L) of pure class distinctions, other agents unless otherwise noted, were of analytical grade. 4.2 Water In line with GB/T 6682 two water requirements. 4.3 Nitrogen A purity of 99.99%. 4.4 Acid Hydrolysis 4.4.1 acid hydrolysis agent. concentrated hydrochloric acid is diluted with water to 6mol/L. 4.4.2 Refrigerant. liquid nitrogen, dry ice or dry ice acetone and ethanol. 4.4.3 diluted with buffer machine. concentrated hydrochloric acid is diluted with water to 0.02mol/L.

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