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GB/T 31270.13-2025 English PDF

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GB/T 31270.13-2025: Test guidelines on environmental safety assessment for chemical pesticides - Part 13: Daphnia sp. acute immobilisation test
Status: Valid

GB/T 31270.13: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB/T 31270.13-2025English359 Add to Cart 4 days [Need to translate] Test guidelines on environmental safety assessment for chemical pesticides - Part 13: Daphnia sp. acute immobilisation test Valid GB/T 31270.13-2025
GB/T 31270.13-2014English279 Add to Cart 3 days [Need to translate] Test guidelines on environmental safety assessment for chemical pesticides -- Part 13: Daphnia sp. acute immobilisation test Valid GB/T 31270.13-2014

Basic data

Standard ID GB/T 31270.13-2025 (GB/T31270.13-2025)
Description (Translated English) Test guidelines on environmental safety assessment for chemical pesticides - Part 13: Daphnia sp. acute immobilisation test
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B15
Classification of International Standard 65.100
Word Count Estimation 18,187
Date of Issue 2025-08-29
Date of Implementation 2026-03-01
Older Standard (superseded by this standard) GB/T 31270.13-2014
Issuing agency(ies) State Administration for Market Regulation, National Standardization Administration

GB/T 31270.13-2025: Test guidelines on environmental safety assessment for chemical pesticides - Part 13: Daphnia sp. acute immobilisation test


---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT31270.13-2025
ICS 65.100 CCSB15 National Standard of the People's Republic of China Replaces GB/T 31270.13-2014 Test Guidelines for Environmental Safety Assessment of Chemical Pesticides Part 13.Daphnia acute immobilization test Released on August 29, 2025 Implementation on March 1, 2026 State Administration for Market Regulation The National Standardization Administration issued

Table of Contents

Preface III Introduction V 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Principle 2 5 Test conditions 2 6 Reagents and Materials 2 7 Instruments and Equipment 3 8 Test and reference substances 3 9 Test Step 3 10 Experimental data processing 5 11 Quality Control 6 12 Test Report 6 Appendix A (Informative) Preparation of Reconstituted Water 8 Appendix B (Normative) Test Water Quality Requirements 10 Reference 11 Preface This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document is Part 13 of GB/T 31270 "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides". GB/T 31270 has been published. The following parts. --- Part 1.Soil metabolism test; --- Part 2.Hydrolysis test; --- Part 3.Photolysis test; --- Part 4.Soil adsorption/desorption test; --- Part 5.Soil leaching test; --- Part 6.Volatility test; --- Part 7.Bioconcentration test; --- Part 8.Water-sediment system metabolism test; --- Part 9.Short-term feeding toxicity test for birds; --- Part 10.Bee acute toxicity test; --- Part 11.Silkworm acute toxicity test; --- Part 12.Fish acute toxicity test; --- Part 13.Daphnia acute immobilization test; --- Part 14.Algal growth inhibition test; --- Part 15.Earthworm acute toxicity test; --- Part 16.Soil microbial toxicity test; --- Part 17.Acute toxicity test for natural enemy Trichogramma; --- Part 18.Acute toxicity test for natural enemies and amphibians; --- Part 19.Non-target plant impact test; --- Part 20.Short-term feeding toxicity test for livestock; --- Part 21.Toxicity test for large crustaceans; --- Part 22.Soil surface photolysis test; --- Part 23.Birds acute oral toxicity test. This document replaces GB/T 31270.13-2014 "Test Guidelines for Environmental Safety Assessment of Chemical Pesticides Part 13.Acute Activity of Daphnia Inhibition test, compared with GB/T 31270.13-2014, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope has been changed (see Chapter 1, Chapter 1 of the.2014 edition); b) Deleted 6 terms and definitions. “test substance”, “chemical pesticide”, “technical product”, “preparation”, “active ingredient” and “reference substance” (see.2014 Chapter 2 of the.2011 edition); c) Added four terms and definitions. “50% inhibition concentration”, “static test method”, “semi-static test method” and “flow-through test method” (see Chapter 3); d) The test conditions have been changed (see Chapter 5, 4.1.6 of the.2014 edition); e) Added the relevant content of reagents (see 6.1); f) The relevant content on test organisms has been changed (see 6.2, 4.1.1 of the.2014 edition); g) Added relevant content on test containers (see 6.3); h) The relevant content of test water has been changed (see 6.4, 4.1.5 of the.2014 edition); i) The relevant content on instruments and equipment has been modified (see Chapter 7, 4.1.4 of the.2014 edition); j) The relevant content of the test substance has been changed (see 8.1, 4.1.2 of the.2014 edition); k) Added requirements for the selection of test methods (see 9.1); l) Added the test solution preparation method (see 9.3.2); m) The methods of exposure, culture, observation and determination have been changed (see 9.3.3, 9.3.4, 9.3.5, and 4.2.2 of the.2014 edition); n) Added concentration detection method (see 9.3.6); o) Added relevant content on reference substances (see 9.5); p) Added content related to data collation (see 10.1); q) The statistical method has been changed (see 10.2, 4.3 of the.2014 edition); r) The quality control conditions have been changed (see Chapter 11, 4.4 of the.2014 edition); s) Changed the test report requirements (see Chapter 12, Chapter 5 of the.2014 edition); t) Added Appendix B (Normative) “Water Quality Requirements for Test Water” (see Appendix B). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Pesticide Standardization (SAC/TC133). This document was drafted by. Pesticide Control Institute, Ministry of Agriculture and Rural Affairs. The main drafters of this document are. Chen Lang, Yang Hairong, Yuan Shankui, Qu Mengmeng, Zhou Xinxin, Bu Yuanqing, Pu Qianyun, An Xuehua, Chen Liping, Xu Weili, Zhou Binbin and Zhang Haiqing. The previous versions of this document and the documents it replaces are as follows. ---First published in.2014 as GB/T 31270.13-2014; ---This is the first revision.

introduction

In order to reduce the impact of pesticide use on the ecological environment and provide data that meets the requirements of pesticide environmental risk assessment, it is necessary to carry out environmental safety assessment. Tests are conducted to clarify the metabolism, degradation, adsorption, leaching, bioaccumulation and other characteristics of pesticides in the environment as well as their toxicity to non-target organisms. GB/T 31270 "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides" is a test method standard guiding the environmental safety evaluation test of pesticides in my country. The standard aims to standardize the pesticide environmental safety evaluation test work and improve the scientificity and comparability of pesticide environmental safety evaluation tests. It consists of 23 parts. constitute. --- Part 1.Soil metabolism test. The purpose is to clarify the metabolic pathways and metabolic rates of pesticides in soil. --- Part 2.Hydrolysis test. The purpose is to clarify the hydrolysis pathway and hydrolysis rate of pesticides. --- Part 3.Photolysis test. The purpose is to clarify the photolysis pathway and photolysis rate of pesticides. --- Part 4.Soil adsorption/desorption test. The purpose is to clarify the soil adsorption characteristics of pesticides. --- Part 5.Soil leaching test. The purpose is to clarify the leaching of pesticides. --- Part 6.Volatility test. The purpose is to clarify the volatility of pesticides. --- Part 7.Bioconcentration test. The purpose is to determine the bioconcentration of pesticides in fish. --- Part 8.Water-sediment system metabolism test. The purpose is to clarify the metabolic pathways and metabolism of pesticides in water-sediment systems. Thanks for the rate. --- Part 9.Short-term feeding toxicity test for birds. The purpose is to determine the short-term feeding toxicity of pesticides to birds. --- Part 10.Bee acute toxicity test. The purpose is to determine the acute toxicity of pesticides to bees. --- Part 11.Acute toxicity test to silkworm. The purpose is to determine the acute toxicity of pesticides to silkworm. --- Part 12.Fish acute toxicity test. The purpose is to determine the acute toxicity of pesticides to fish. --- Part 13.Daphnia acute activity inhibition test. The purpose is to determine the acute toxicity of pesticides to Daphnia. --- Part 14.Algal growth inhibition test. The purpose is to clarify the toxicity of pesticides to algae. --- Part 15.Earthworm acute toxicity test. The purpose is to determine the acute toxicity of pesticides to earthworms. --- Part 16.Soil microbial toxicity test. The purpose is to clarify the toxicity of pesticides to soil microorganisms. --- Part 17.Acute toxicity test for natural enemy Trichogrammatis. The purpose is to clarify the toxicity of pesticides to Trichogrammatis. --- Part 18.Acute toxicity test for natural enemies and amphibians. The purpose is to clarify the acute toxicity of pesticides to amphibians. --- Part 19.Non-target plant effects test. The purpose is to clarify the toxicity of pesticides to non-target plants. --- Part 20.Short-term feeding toxicity test for livestock. The purpose is to clarify the feeding toxicity of pesticides to livestock. --- Part 21.Toxicity test to large crustaceans. The purpose is to determine the toxicity of pesticides to large crustaceans. --- Part 22.Soil surface photolysis test. The purpose is to clarify the photolysis pathway and photolysis rate of pesticides on the soil surface. --- Part 23.Bird acute oral toxicity test. The purpose is to determine the acute oral toxicity of pesticides to birds. Test Guidelines for Environmental Safety Assessment of Chemical Pesticides Part 13.Daphnia acute immobilization test Warning. People who use this document should have practical experience in regular laboratory work. This document does not address all possible safety issues. The user is responsible for taking appropriate safety and health measures and ensuring that the conditions stipulated by relevant national laws and regulations are met.

1 Scope

This document describes the test method for the acute inhibition of immobilization of Daphnia pulex for the environmental safety evaluation of chemical pesticides. This document applies to the acute immobilization inhibition test on Daphnia pulex conducted for the registration of chemical pesticides.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. NY/T 3273 Guidelines for Aquatic Toxicity Testing of Refractory Pesticides NY/T 4195.5 Pesticide Registration Environmental Impact Test Biological Material Cultivation Part 5.Daphnia magna

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 In the acute activity inhibition test, the concentration of the test substance that causes 50% of the test daphnia to inhibit their activity is expressed as EC50. Note. The units are mga.i./L and mg test substance/L. 3.2 Immobilization Gently shake the test container; the Daphnia cannot swim within 15 s (slight movement of the appendages is allowed). 3.3 Static test method statictest A poisoning method in which the drug solution is not changed during the test. 3.4 Semi-static test method semi-static test The poisoning method is to replace the drug solution at regular intervals (such as 24 hours) during the test. 3.5 flow-through test The method of continuously updating the drug solution during the test.

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