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GB/T 30990-2014: Determination of the activity of lysozyme
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| GB/T 30990-2014 | English | 169 |
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Determination of the activity of lysozyme
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GB/T 30990-2014
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Basic data | Standard ID | GB/T 30990-2014 (GB/T30990-2014) | | Description (Translated English) | Determination of the activity of lysozyme | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G60 | | Classification of International Standard | 07.080 | | Word Count Estimation | 7,769 | | Date of Issue | 7/24/2014 | | Date of Implementation | 3/1/2015 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standards Bulletin No. 19, 2014 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for detecting the activity of lysozyme. This standard applies to biological and chemical reagents, measured activity in different industrial products and raw materials lysozyme. | GB/T 30990-2014: Determination of the activity of lysozyme---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of lysozyme
ICS 07.080
G60
National Standards of People's Republic of China
Lysozyme activity detection method
Issued on. 2014-07-24
2015-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard biochemical tests by the National Standardization Technical Committee (SAC/TC387) and focal points.
This standard was drafted. Shenzhen Academy of Metrology and Quality Inspection, China Testing Technology Research Institute, Zhuhai Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Lai Xiaofang, Tan peace, Lin Lin, Sun Dengfeng, Lai Xintian, blue whole school, Yangguo Wu, Li Italy, Li Xi, Pan Lanfang,
Ze Mo Qiuhua.
Lysozyme activity detection method
1 Scope
This standard specifies the method for detecting the activity of lysozyme.
This standard applies to biological and chemical reagents, measured activity in different industrial products and raw materials lysozyme.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Lysozyme lysozyme
Grape glucosidase, through its active site peptide chain of Glu35 and Asp52 residues catalyzed hydrolysis of bacterial cell walls
β- glycosidic linkage N- acetylmuramic acid and N- acetylglucosamine C1 C4 is the so rapidly dissolved in the bacterial cell wall polysaccharides
ingredient.
Note. also known as immunoglobulin G, muramidase, N- acetyl xylan murein hydrolase.
3.2
Lysozyme activity lysozymeactivity
At 25 ℃, 0.1mol/L phosphate buffer (pH6.2) in order to dissolve the wall of Micrococcus as the substrate, so that the absorbance at 450nm at every minute
Value decrease the amount of enzyme required 0.001. Activity units of U/mg or U/g or U/mL.
Principle 4
The detection method to dissolve the wall of Micrococcus (Micrococcuslysodeikticus) as a reaction substrate, which catalyzes the hydrolysis of cells using lysozyme
Wall, bacterial cell wall due to the loss of protection can not maintain osmotic balance within the cell death and lysis, resulting bacterial suspension turbidity decreased by purple
Outside - visible spectrophotometer measuring the change in absorption spectrophotometry at 450nm and value in terms of the activity of the sample units.
5 equipment and appliances
5.1 biological safety cabinet.
5.2 Autoclave.
5.3 shaker. (37 ± 1) ℃.
5.4 Incubator. (37 ± 1) ℃.
5.5 Centrifuge. up to 12000g/min.
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