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GB/T 30664-2014: Chemicals -- Amphibian metamorphosis assay
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Chemicals -- Amphibian metamorphosis assay
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GB/T 30664-2014
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Basic data | Standard ID | GB/T 30664-2014 (GB/T30664-2014) | | Description (Translated English) | Chemicals -- Amphibian metamorphosis assay | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 13.020 | | Word Count Estimation | 36,341 | | Date of Issue | 12/31/2014 | | Date of Implementation | 4/30/2015 | | Quoted Standard | ASTM E729; ASTM E1439 | | Regulation (derived from) | National Standards Bulletin 2014 No. 33 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemical test methods amphibian metamorphosis principle, the method described, test procedures, data and reports. This standard applies to chemical testing amphibian metamorphosis. | GB/T 30664-2014: Chemicals -- Amphibian metamorphosis assay---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals - Amphibian metamorphosis assay
ICS 13.300; 13.020
A80
National Standards of People's Republic of China
Chemicals amphibian metamorphosis test
Issued on. 2014-12-31
2015-04-30 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Table of Contents
Introduction Ⅴ
1 Scope 1
2 Normative references 1
3 Abbreviations 1
4 principle of the method 1
Method Description 2 5
5.1 Test species 2
5.2 Equipment and Supplies 2
5.3 Chemicals detectable 2
5.4 exposure system 3
5.5 Water 3
5.6 test water in the iodide concentration 3
5.7 Animal Feeding 3
5.7.1 adult breeding 3
5.7.2 tadpole rearing and select 4
5.7.3 tadpoles culture and feeding 4
5.8 Analytical Chemistry 5
5.9 chemical delivery 5
Select test concentration 5.10 5
Step 6 6 Test
6.1 Test start and for six
6.2 Observation 7
6.2.1 General Description 7
6.2.2 measured on Day 7 7
6.2.3 measured on day 21 (test termination) 7
6.3 Determination of biological endpoints 8
6.4 endpoint 8
6.4.1 General description 8
6.4.2 Development Stage 8
6.4.3 HLL 9
6.4.4 body length and wet weight 9
6.4.5 Thyroid histology 9
6.4.6 Death 10
6.4.7 More Observation 10
7 Data and Reports 10
7.1 Data Collection 10
7.1.1 Test substance 10
7.1.2 Test conditions 10
10 7.1.3 Results
7.1.4 Data Report 10
7.2 Performance criteria and test acceptability/effectiveness 11
7.3 Test validity 11
7.4 AMA decision logic 11
7.4.1 AMA decision logic process 11
7.4.2 Early development (through developmental stage, SVL and HLL judgment) 12
7.4.3 are not synchronized development (determined using developmental stage standard) 13
7.4.4 Histopathology 13
7.4.5 developmental delay (in the developmental stage, HLL, body wet weight, SVL determination) 13
7.5 Statistical Analysis 14
Special considerations 15 7.6 Data Analysis
7.6.1 have an adverse effect using test concentration of 15
7.6.2 solvent control 15
60 7.6.3 and higher stage of development processing group 16
Test conditions Annex A (informative) 21 days 17 amphibian metamorphosis trial
Annex B (informative) raw data and report summary data table 18
Annex C (informative) with one or more late development tadpole number concentration and body weight of more than 20% is long
Analysis of alternatives 29
References 30
Hindlimb morphology drawing stage 151 Xenopus laevis tadpoles 7
Length measuring 2 Xenopus laevis tadpoles and HLL measuring 9
Figure 3 AMA decision logic 12
Statistical test method of Figure 4 Effect of continuous data flow diagram 15
Table 1 water conditions AMA during Xenopus laevis tadpoles to survive Feeding Programme 4
According to Table 2 highlight morphological stage Neuwkoop and Faber guidance signs 6
Table 3 AMA observation point in time the primary endpoint 8
Table 4 AMA performance standards 11
Test conditions Table A.1 21 Tian amphibian metamorphosis Test 17
General Information Table B.1 test chemicals 18
Table B.2 7 and 21 days of raw data collection form 18
Table B.3 7 and calculate the first 21 days of the end of the data summary 21
22 Table B.4 daily mortality data
23 Table B.5 water quality standards
Table 23 summarizes the chemical data B.6
Table B.7 core standard histopathological report forms 24
Table B.8 other histopathological criteria 25
Table B.9 histopathologic findings narrative description 26
Table B.10 AMA 7 days or 21 days Summary Report 27 Table Template
The table below summarizes the report template Table B.11 AMA day 7 or day 21 of the 28 stages of development data
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The Standards and Organisation for Economic Co-operation and Development (OECD) guidelines for testing of chemicals No.231 (2009 Nian) "amphibian metamorphosis Test Review
Price "agreement (in English) technical content.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Research Center for Eco-Environmental Sciences, Chinese Academy of.
The main drafters of this standard. Cheng Yan, Qin Zhanfen, Cui Yuan, Falls Apart, Li Xi, Zhang Yinfeng, Xie Wenping.
Chemicals amphibian metamorphosis test
1 Scope
This standard specifies the chemical test methods amphibian metamorphosis principle, the method described, test procedures, data and reports.
This standard applies to chemical testing amphibian metamorphosis.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Standard Guide ASTME729 with fish, macroinvertebrates and amphibians were tested material acute toxicity test
(Standardguideforconductingacutetoxicitytestsontestmaterialswithfishes, macroinvertebrates,
andamphibians)
ASTME1439 Xenopus embryos Standard Guide teratogenicity test (FETAX) [Standardguideforconductingthe
frogembryoteratogenesisassay-Xenopus (FETAX)]
NieuwkoopPD, FaberJ Xenopus Growth Table (NormaltableofXenopuslaevis)
3 Abbreviations
The following abbreviations apply to this document.
AMA. amphibian metamorphosis test (amphibianmetamorphosisassay).
HPT. hypothalamic - pituitary - thyroid (hypothalamic-pituitary-thyroid).
SVL. mouth to cloaca length (snouttoventlength).
hCG. human chorionic gonadotropin (humanchorionicgonadotropin).
DO. Dissolved oxygen (dissolvedoxygen).
MTC. the maximum tolerated concentration (maximumtoleratedconcentration).
LC50. lethal concentration (lethalconcentration50).
TSH. thyroid stimulating hormone (thyroidstimulatinghormone).
HLL. hindlimb length (hindlimblength).
OECD. Organization for Economic Cooperation and Development (organisationforeconomicco-operationanddevelopment).
NF. NieuwkoopPD, FaberJ (1994) on the normal development of Xenopus laevis morphological criteria developmental stage classification (Nieuwk-
oopandFaber).
4 principle of the method
4.1 AMA is a screening test, its purpose is to empirically identify possible substances interfere with the normal function of the HPT axis. In HPT axis Paul
Keep within the scope of the structure and function, AMA represents a generalized vertebrate model approach. This is an important trial because two
Amphibians metamorphosis is a study of more mature, thyroid-dependent process that HPT axis of the active substance within the responsive and
This is currently the only active thyroid test animals detect morphological development.
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