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GB/T 29585-2013: Detection and identification of Anguina agrostis (Steinbuch) Fillpjev
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Detection and identification of Anguina agrostis (Steinbuch) Fillpjev
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GB/T 29585-2013
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Basic data | Standard ID | GB/T 29585-2013 (GB/T29585-2013) | | Description (Translated English) | Detection and identification of Anguina agrostis (Steinbuch) Fillpjev | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 11,14 | | Quoted Standard | SN/T 2122 | | Regulation (derived from) | National Standards Bulletin 2013 No. 10 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies: bentgrass grain nematodes quarantine and identification methods. This standard applies to seeds and other grains bentgrass plants quarantine and identification of nematodes. | GB/T 29585-2013: Detection and identification of Anguina agrostis (Steinbuch) Fillpjev---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Anguina agrostis (Steinbuch) Fillpjev
ICS 65.020.01
B16
National Standards of People's Republic of China
Bentgrass nematode quarantine and identification of grain
DetectionandidentificationofAnguinaagrostis (Steinbuch) Filipjev
Issued on. 2013-07-19
2013-12-06 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Qingdao, People's Republic of China Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Wang Jincheng, Yang Xiuli, Cui Tiejun, Zhang Zhiyu, Liu Peng, Liu Fang, Zhang Yujun, NIU Chun King.
Bentgrass nematode quarantine and identification of grain
1 Scope
This standard specifies the quarantine and identification of bentgrass grain nematode.
This standard applies to seeds and other plant material in grain bentgrass nematode quarantine and identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 bentgrass nematode grain basic information
Chinese name. bentgrass grain nematodes.
English name. bentgrassnematode.
Scientific name. Anguinaagrostis (Steinbuch, 1799) Filipjev, 1936.
Taxonomic position. door nematode (Nematoda), side tail gland Gang (Secernentea), Tylenchida (TylenchidaThorne, 1949), mitochondria
Beetles [AnguinidaeNicol, 1935 (1926)], grain nematode genera (AnguinaScopoli, 1777).
Parasite seeds, galls, plant disability organizations is the main carrier particle bentgrass nematode spread.
For additional information, see Appendix bentgrass grain nematode A.
4 principle of the method
Field testing to obtain samples by microscopy and molecular biology methods to obtain information identifying characteristics and nucleic acid form, were identified.
5 instruments, appliances
5.1 Instrument. a microscope (100 × ~ 1000 ×, having ocular micrometer or a plotter, the image capture device, measurement software), stereo microscope
(10 × ~ 80 ×, a transmission light source), refrigerator, PCR instrument, electrophoresis, gel imaging system, low-speed centrifuge (1000r/min ~ 5000r/min), pure
Water meter.
5.2 Appliances. pick a needle nematodes, slides, cover slips, alcohol lamp, pipettes (1000μL, 100μL, 10μL, 2.5μL), clock face dish
(Φ7cm, φ9cm), dish (φ7cm, φ9cm), trays, sets sieve (400 mesh, 500 mesh), improved Berman funnel, heating plate, drilling
, A scalpel.
6 Reagents
Unless otherwise indicated in the analysis use only reagents or confirmed analytically pure distilled water.
Slide mountant 6.1, 4% formaldehyde solution and paraffin.
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