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GB/T 29577-2013 English PDF

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GB/T 29577-2013: Detection and identification of Ditylenchus destructor Thorne
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Basic data

Standard ID GB/T 29577-2013 (GB/T29577-2013)
Description (Translated English) Detection and identification of Ditylenchus destructor Thorne
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 16,140
Quoted Standard SN/T 1141-2002; SN/T 1157; SN/T 1158; SN/T 2122
Regulation (derived from) National Standards Bulletin 2013 No. 10
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies: stem rot nematode (Ditylenchus destructor Thorne) quarantine and identification methods. This standard applies to parts of vegetables and flowers roots tubers, bulbs, corms, etc., other host plants, seeds, stems, leaves and rottin

GB/T 29577-2013: Detection and identification of Ditylenchus destructor Thorne

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Detection and identification of Ditylenchus destructor Thorne ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine and identification of stem rot nematode Issued on. 2013-07-19 2013-12-06 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Jiang Lihui, Li Yinong, Wang Wanchun, Lifang Rong, Zhao history, Lv Yufeng, Han Jing, Hai, Gao Na, while Yong. Quarantine and identification of stem rot nematode

1 Scope

This standard specifies the stem rot nematode (DitylenchusdestructorThorne) of quarantine and identification methods. This standard applies to roots part tubers, bulbs, corms and other vegetables and flowers, and other host plants seeds, stems, leaves and planting medium Quarantine and identification of stem rot nematode.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1141-2002 Ditylenchus quarantine and identification of nematodes SN/T 1157 export plant seedlings quarantine rules SN/T 1158 bonsai plant quarantine entry and exit procedures SN/T 2122 entry and exit quarantine of plants and plant products sampled

3 stem rot nematode basic information

Chinese name. Ditylenchus destructor; potato rot nematode stem. English name. potatorotnematode; tuberrotnematode. Scientific name. DitylenchusdestructorThorne, 1945. Stem rot nematode belonging nematode door Nematoda (Rudolphi, 1808) Lankester, 1877, gang-side end gland Secernenteavon Linstow, 1905, Tylenchida TylenchidaThorne, 1949, grain nematode families AnguinidaeNicol, 1935 (1926), stem nematode genera DitylenchusFilipjev, 1936. Stem rot nematodes spread over long distances by the block Ballet, bulbs, corms and growing media host plants. For additional information, see Appendix D. destructor in A.

4 principle of the method

Acquisition suspected nematode host plant material separation, observed with a microscope isolated nematodes, according to stem rot nematode females and males The morphological characteristics of morphological identification, molecular biology double PCR detection method is an important auxiliary means of identification of stem rot nematode.

5 Apparatus appliances

5.1 Instrument Biological microscope (100 × ~ 1000 ×), stereo microscope (10 × ~ 80 ×, a transmission light source), refrigerator, electric oven, PCR instrument, Electrophoresis, gel imaging system, low-speed centrifuge (1000r/min ~ 5000r/min), water meter. 5.2 Appliances Improved separator funnel, pipette (10μL, 25μL, 100μL, 1000μL); other appliances see SN/T 1141-2002 in

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