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GB/T 28942-2012: Sampling procedure of vector infected by pathogens -- Flea
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 28942-2012 | English | 169 |
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Sampling procedure of vector infected by pathogens -- Flea
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GB/T 28942-2012
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Basic data | Standard ID | GB/T 28942-2012 (GB/T28942-2012) | | Description (Translated English) | Sampling procedure of vector infected by pathogens -- Flea | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C05 | | Classification of International Standard | 11.020 | | Word Count Estimation | 7,750 | | Regulation (derived from) | National Standards Bulletin No. 31 of 2012 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the live fleas infected with pathogens sampling procedures. This standard applies to a variety of communicable diseases, infections or potentially infected fleas pathogens sampled. | GB/T 28942-2012: Sampling procedure of vector infected by pathogens -- Flea---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Sampling procedure of vector infected by pathogens. Flea
ICS 11.020
C05
National Standards of People's Republic of China
Vector biological pathogens flea sampling procedures
Issued on. 2012-11-20
2013-05-01 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard drafting of the main unit. the PLA Academy of Military Medical Institute of Microbiology and Epidemiology, Disease Control and Prevention in China
Heart diseases prevention and control, the Beijing Municipal Center for Disease Control and Prevention.
The main drafters of this standard. Zhao Tong Yan, Zhang Yingmei, Germany Dong Yan, Lu Liang, Zhang Yong.
Vector biological pathogens flea sampling procedures
1 Scope
This standard specifies the live fleas infected with pathogens sampling procedures.
This standard is applicable to many communicable diseases, fleas infected or potentially infected samples of pathogens.
2 reagent material and equipment
Ether, dry ice, ice, squirrel cage, rat trap, sticky paper fleas, flea probe stick, enamel plates, ophthalmic forceps, rat bags, vials, bag, ice box, liquid nitrogen tank,
Deep freezer (-80 ℃).
3 steps
3.1 specimen collection
3.1.1 Animal fleas collected
Using the mouse or squirrel cage clamp method were collected animals. After the rat trap to capture the animal is removed, into rat bags, tie the rat bags; squirrel cage captive animals
Mounted directly on the mouse bag mouse bags truss, back to the lab, in a confined space with ether anesthesia, the animals were put in bags and mouse enamel dish, from
Animal body surface and the surface of rat bags look carefully fleas.
3.1.2 animal nest flea collection
Dig to take a host nest of grass clippings and other contents and surface dust, into the rat bags, back to the lab, anesthesia, you can take the following methods of which
A treatment.
After a) water into the pan, the water you have not seen the contents of at least 5cm, the waste will be picked, after the agitation of the water, so that float on the water fleas
Pick up fleas;
b) directly from the contents of the investigation fleas.
3.1.3 burrow flea collection
Penetrating flea stick (production method see Appendix A) extends into the hole in the pull dry, remove the sticky substance fleas will probe into the rat flea stick on bags, back test
After picking fleas anesthesia chamber.
3.1.4 ground free flea collection
Daily 1h ~ 2h before dusk flea placed sticky paper (production method see Appendix A) in the room corner, recovered the next day at dawn, seized adhesion
Flea.
3.2 specimens are preserved
The fleas collected on the basis of collected host dispensed in vials, and serial number, name specimens detailed records of the number of collection sites, raw
Habitat information, time, people and collected a host animal names, etc. (see Appendix B). Number of vials and then bag installed, and the bags are numbered,
Detailed records of the number of vials in the bag case.
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