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GB/T 28630.1-2012: Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe
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| GB/T 28630.1-2012 | English | 299 |
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Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe
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GB/T 28630.1-2012
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Basic data | Standard ID | GB/T 28630.1-2012 (GB/T28630.1-2012) | | Description (Translated English) | Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 13,144 | | Quoted Standard | GB/T 28630.4-2012 | | Regulation (derived from) | National Standards Bulletin 2012 No. 17 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the spot syndrome nucleic acid probe dot blot method requires reagents and materials, equipment, procedures, and results of determination. This section applies to shrimp as shrimp, shrimp, larvae, larvae and eggs of live, chilled o | GB/T 28630.1-2012: Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS65.020.30
B41
National Standards of People's Republic of China
(WSD) diagnostic protocols spot syndrome
Part 1. nucleic acid probe dot blot hybridization assay
Part 1. DotblothybridizationmethodwithDNAprobe
Issued on. 2012-11-01 2012-07-31 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 28630 "white spot syndrome (WSD) diagnostic protocols" is divided into five parts.
--- Part 1. nucleic acid probe dot blot hybridization assay;
--- Part 2. nested PCR assay;
--- Part 3. In situ hybridization assay;
--- Part 4. Histopathological diagnosis method;
--- Part 5. TE fresh tissue staining.
This is Part 1 GB/T 28630 in.
Rules in this section in accordance with GB/T 1.1-2009 given draft.
This section proposed by the People's Republic of China Ministry of Agriculture.
This part of the National Standardization Technical Committee on Fisheries (SAC/TC156) centralized.
This section drafted by. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Ministry of Agriculture, the National Fisheries Extension Center.
The main drafters of this section. Huang Jie, Yang Bing, Chen Aiping, Song Xiaoling, the history of the silver, Yang Cong Hai, Wei Qi, Liu Li.
Introduction
When issuing authority of the document drew attention to the statement of compliance with this document, related to the national invention patent "shrimp epidemic pathogen detection reagent
Kit and detection method "(ZL00111336.4) use.
The issuing authority for the patent document authenticity, validity and scope without any position.
The patent holder has to ensure that the issuing authority of this document, they are willing to work with any applicant reasonable and non-discriminatory terms and conditions
Under patent license negotiations. The patent holder's statement has been filed in the release mechanism of the present document. Related information can be
Contact the following ways.
Patent Holder Name. History of the silver, Yang Bing, Huang Jie, Song Xiaoling Yang Cong sea.
Address. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Nanjing Road, Qingdao City, Shandong Province, No. 106.
Please note that in addition to the above patents, some of the contents of this document may still involve patents issuing authority of this document does not undertake to recognize these special
Lee responsibility.
(WSD) diagnostic protocols spot syndrome
Part 1. nucleic acid probe dot blot hybridization assay
1 Scope
This section GB/T 28630 provisions of the White Spot Syndrome DNA probe dot blot hybridization methods needed reagents and materials, equipment,
Procedure and result judgment.
This section applies to shrimp as shrimp, young shrimp, shrimp larvae, juveniles and fertilized in vivo, chilled or frozen products and other white spot syndrome
Virus sensitive crustaceans White Spot Syndrome pathogen screening, clinical diagnosis of the condition, the estimate does not apply to active viral infection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated edition applies to this article
Member. Where not dated references, the latest edition (including any amendments) applies to this document.
Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method
Principle 3
Pathogens and pathological features of White Spot Syndrome 3.1
See GB/T 28630.4-2012 2.1.
3.2 Technical Principle
In non-radioactive marker --- digoxigenin labeled White Spot Syndrome Virus (WSSV) DNA fragment syndrome, or nucleic acid probes, and present in the
Samples of viral DNA specificity of nucleic acid hybridization, by an antigen - antibody reaction and enzyme - substrate chemical color reaction like display
Products in the presence of a virus.
4 Reagents and materials
4.1 Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity.
4.2 chlorine disinfectant. medical supplies or aquatic drugs.
4.3 Hybrid Membrane. nitrocellulose membrane.
4.4 PCRDIGProbeSynthesisKit1).
4.5 Sodium lauryl sarcosine (Sarkosyl). chemical reagents.
4.6 sodium dodecyl sulfate (SDS). chemical reagents.
4.7 disodium edetate (EDTA-Na2 · 2H2O). chemical reagents.
4.8 mercaptoethanol. biochemical reagents.
4.9 Tris balance phenol (saturated phenol). biochemical reagents.
1) PCRDIGProbeSynthesisKit is provided by Roche, the trade name of the product. This information is given in order to facilitate the use of this section
Who does not imply endorsement of the product. If other equivalent product has the same effect, you can use these equivalent products.
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