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GB/T 28100-2011 English PDF

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GB/T 28100-2011: Detection and identification of Burkholderia caryoph ylli (Burkholder) Yabuuchi et al.
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Basic data

Standard ID GB/T 28100-2011 (GB/T28100-2011)
Description (Translated English) Detection and identification of Burkholderia caryoph ylli (Burkholder) Yabuuchi et al.
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 14,174
Date of Issue 2011-12-30
Date of Implementation 2012-06-01
Quoted Standard GB/T 4789.28; SN/T 1157
Regulation (derived from) Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the carnation bacterial Kwai listless quarantine and identification of bacteria. This standard applies to Dianthus, baby's breath and other plant seedlings and products of bacterial wilt of carnation quarantine and identification of bacteria.

GB/T 28100-2011: Detection and identification of Burkholderia caryoph ylli (Burkholder) Yabuuchi et al.


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Detection and identification of Burkholderia caryoph ylli (Burkholder) Yabuuchi et al. ICS 65.020.01 B16 National Standards of People's Republic of China Carnation bacterial wilt pathogen identification of quarantine (Burkholder) Yabuuchietal. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Shandong Entry-Exit Inspection and Quarantine of Liaoning People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Shao Xiuling, Wangyou Fu, Zhao Wenjun, Ganqin Hua, Li Yan, Wang Premiership, Nepal Suki, Su Zhi Ping, Wu Xinghai, Zhang Zhiyu, Yu Dongdong. Carnation bacterial wilt pathogen identification of quarantine

1 Scope

This standard specifies the method for inspection and identification carnation bacterial wilt pathogen. This standard applies to quarantine and identification of Dianthus, stars and other plant seedlings and products Carnation bacterial wilt pathogen.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 4789.28 Microbiological examination of food hygiene staining, media and reagents SN/T 1157 export plant seedlings quarantine rules

3 Carnation bacterial wilt pathogen basic information

Chinese name. Carnation bacterial wilt pathogen. Scientific name. Burkholderiacaryophylli (Burkholder1942) Yabuuchietal.1993 Pseudomonascaryophylli (Burkholder1942) Starr Chinese common name. Burkholderia carnation, carnation Burkholderia, Anthurium Bacterial Leaf Blight Disease English. bacterialwiltofcarnation. Genus Prokaryotae (Procaryotae) thin Bi bacterial phyla (Gracilicutes) dark bacteria Gang (Scotophobia) Pseudomonas mesh (Pseud- omonadales) Pseudomonas Branch (Pseudomonadaceae) Burkholderia (Burkholderia). By cutting a part of bacteria infection spread short distances. Carnation bacterial wilt pathogen base his information, see Appendix A.

4 principle of the method

The field inspection, laboratory microscopes, quantitative PCR instrument and other equipment on the samples tested, according to colony characteristics of pathogens, Cell morphology, physiological and biochemical characteristics and pathogenicity and molecular biology test results to determine.

5 equipment and appliances

5.1 Equipment Electronic balance (inductance 0.0001g), plant growth incubator, incubator, temperature oscillation incubator, autoclave, laminar flow Taiwan, biological microscope (with camera system), high-speed refrigerated centrifuge (12000r/min), a small centrifuge, water meter, temperature water bath, cold Refrigerator, PCR instrument, electrophoresis, electrophoresis tank gel image analyzer, electric mixer, vacuum freeze-drying machine. 5.2 Appliances Petri dishes, test tubes, adjustable micro-injector (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL) and tip, mortar,

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