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GB/T 28099-2011 English PDF

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GB/T 28099-2011: Detection and identification of Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 28099-2011	English	229	Add to Cart	3 days [Need to translate]	Detection and identification of Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.	Valid	GB/T 28099-2011

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Basic data

Standard ID	GB/T 28099-2011 (GB/T28099-2011)
Description (Translated English)	Detection and identification of Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	B16
Classification of International Standard	65.020.01
Word Count Estimation	10,188
Date of Issue	2011-12-30
Date of Implementation	2012-06-01
Quoted Standard	SN/T 0800.1; SN/T 2122
Regulation (derived from)	Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary	This standard specifies the rice bacterial leaf spot pathogen detection and identification methods. This standard applies to rice seeds and plants rice bacterial leaf spot pathogen detection and identification.

GB/T 28099-2011: Detection and identification of Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas oryzae pv.oryzicola (Fang et al.) Swings et al. ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine and identification of rice bacterial leaf spot pathogen DetectionandidentificationofXanthomonasoryzaepv.oryzicola (Fangetal.) Swingsetal. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Xiamen People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine People's Republic of China, Chongqing, Nanjing Agricultural University. Drafters of this standard. Yi Jianping, Lin Shiming, Zhou Guoliang, millet cold, India Liping, Kongde Ying, Xu Zhigang, Hu Whitehead. Quarantine and identification of rice bacterial leaf spot pathogen

1 Scope

This standard specifies the method for the detection and identification of rice bacterial leaf spot pathogen article. This standard applies to rice seeds and the plants detection and identification of rice bacterial leaf spot pathogen.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 0800.1 and export grain, feed inspection sampling and sample preparation methods SN/T 2122 entry and exit quarantine of plants and plant products sampled

3 Basic information of germs rice bacterial leaf streak

Chinese name. rice bacterial leaf spot pathogen (rice raw rice Xanthomonas pv). Scientific name. Xanthomonasoryzaepv.oryzicola (Fangetal, 1956.) Swingsetal, 1990, referred to Xoc.. Synonyms. Xanthomonascampestris pv.oryzicola (. Fangetal, 1956) Dye1978; Xanthomonas oryzicola (Fangetal, 1956.) Dowson1943;. Xanthomonastranslucensf.sp.oryzicola (Fangetal, 1956) Bradbury1971. Disease English. bacterialleafstreakofrice. Spp community Bacteria, Proteobacteria door Proteobacteria, γ- Proteobacteria Gang Gammaproteobacteria, Xanthomonas Head Xanthomonodales, Xanthomonas Branch Xanthomonodaceae, Xanthomonas Xanthomonas (Dowson, 1939). Mainly in the disease germs and disease grass seed winter, followed by regeneration of rice seedlings in the winter or hexandra other weeds. Bacteria mainly by rainwater, stream Water spread contaminated seed is one of the main long-distance transmission. Rice bacterial leaf spot pathogen additional information, see Appendix A.

4 principle of the method

Detection and identification based on symptoms characteristic colony morphology, physiological and biochemical characteristics, PCR reactions and pathogenicity test results, etc.

5 Reagents

Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. Tris (hydroxymethyl) aminomethane hydrochloride (Tris-HCl), ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), hexadecyl Trimethyl ammonium bromide (CTAB), chloroform, isoamyl alcohol, ethanol, protease, ethidium bromide, yeast extract, agar, peptone, beef extract, camp Yang broth, glucose, sucrose, sodium glutamate. Media and reagents formulation in Appendix B.

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