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GB/T 28096-2011 English PDF

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GB/T 28096-2011: Detection and identification of Xanthomonas axonopodis pv. manihotis (Bondar) Vauterin et al.
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GB/T 28096-2011English259 Add to Cart 3 days [Need to translate] Detection and identification of Xanthomonas axonopodis pv. manihotis (Bondar) Vauterin et al. Valid GB/T 28096-2011

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Basic data

Standard ID GB/T 28096-2011 (GB/T28096-2011)
Description (Translated English) Detection and identification of Xanthomonas axonopodis pv. manihotis (Bondar) Vauterin et al.
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 11,177
Date of Issue 2011-12-30
Date of Implementation 2012-06-01
Quoted Standard GB/T 4789.28
Regulation (derived from) Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the cassava plant quarantine in quarantine bacterial wilt pathogen identification methods. This standard applies to cassava seeds, seedlings, seed and other propagating material stems of cassava bacterial wilt pathogen quarantine and identification.

GB/T 28096-2011: Detection and identification of Xanthomonas axonopodis pv. manihotis (Bondar) Vauterin et al.


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Detection and identification of Xanthomonas axonopodis pv. Manihotis (Bondar) Vauterin et al. ICS 65.020.01 B16 National Standards of People's Republic of China Cassava bacterial wilt pathogen identification of quarantine (Bondar) Vauterinetal. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Hainan Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Shandong Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Li Weidong, Feng Liping, Liu Fuxiu, Han Yuchun, Zhou first Chao, Su Han, Wu Cuiping, the king of the Premiership. Cassava bacterial wilt pathogen identification of quarantine

1 Scope

This standard specifies the phytosanitary cassava bacterial wilt pathogen of quarantine and identification methods. This standard applies to cassava seeds, seedlings, stems and other types of inspection and identification of propagation material in cassava bacterial wilt pathogen.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 4789.28 Microbiological examination of food hygiene staining, media and reagents

3 cassava bacterial wilt pathogen basic information

Chinese name. cassava bacterial wilt pathogen (carpet grass Xanthomonas cassava pv). Scientific name. Xanthomonas axonopodis pv.manihotis (Bondar) Vauterin, Hoste, Kersters Swings1995. Synonyms. BacillusmanihotisArthaud-BerthetandBondar1912, Phytomonasmanihotis (Berthetand Bondar) Viegas, Xanthomonasmanihotis (BerthetandBondar) Starr1946, Xanthomonas.campestris pv.manihotis (ArthaudandBondar) Dye1978. Disease English name. bacterialblightofcassava, cassavabacterialblight. Genus Prokaryotae Procaryotes, proteobacteria door Proteobacteria, γ- Proteobacteria Gang Gammaproteobacteria, yellow Aeromonas mesh Xanthomonadales, yellow Aeromonas Branch Xanthomonadaceae, yellow Xanthomonas Xanthomonas, yellow grass carpet Aeromonas spp Xanthomonasaxonopodis. The main long-distance transmission of infection by contaminated seed, seedlings and seed stems. The vast majority of cassava seeds do not show any symptoms of the disease, the bacteria to break Sleep state exists in seed embryo and seed coat. For additional information, see Appendix cassava bacterial wilt pathogen A.

4 principle of the method

The disease symptoms, and biological characteristics of bacteria, physiological and biochemical characteristics, specific PCR test to detect pathogenic and genomic DNA is the Pathogen detection based on identification.

5 apparatus and appliances

5.1 Instrument Electronic balance, stereoscopic microscope, clean benches, temperature incubator, Autoclave, Microbial Identification System, PCR instrument, electrophoresis , Ultraviolet detector or gel imaging systems, centrifuges, constant temperature water bath (30 ℃ ~ 95 ℃), ultra-low temperature freezer, ordinary refrigerator, water machine, eddy Spin oscillator, adjustable micro pipette.

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