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GB/T 28082-2011: Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf.
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Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf.
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GB/T 28082-2011
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PDF similar to GB/T 28082-2011
Basic data | Standard ID | GB/T 28082-2011 (GB/T28082-2011) | | Description (Translated English) | Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf. | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 13,112 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-06-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the elm Fusarium were isolated and cultured, morphological identification methods. This standard applies to Fusarium oxysporum from elm countries and regions have occurred Ulmus (Ulmus L.) seedlings, timber, wood products and wood packaging elm Fusarium quarantine and identification. | GB/T 28082-2011: Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf.
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf.
ICS 65.020.01
B16
National Standards of People's Republic of China
Elm quarantine and identification of Fusarium oxysporum
andOphiostomaulmi (Buisman) Nannf.
Issued on. 2011-12-30
2012-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. Chinese Academy of Inspection and Quarantine.
Drafters of this standard. Wu Shan goods, Yan Jin, Du Hongzhong.
Elm quarantine and identification of Fusarium oxysporum
1 Scope
This standard specifies the elm wilt pathogen isolation and culture, morphological identification method.
This standard applies to happen Fusarium oxysporum from elm countries Ulmus (UlmusL.) Nursery stock, logs, wood and wood packaging
The elm quarantine and identification of Fusarium oxysporum f.
2 Elm Fusarium oxysporum basic information
Chinese name. Yu Fusarium.
Name. Ophiostomanovo-ulmiBrasier;
Ophiostomaulmi (Buisman) Nannf ..
Synonyms. Ceratocystisulmi (Buism.) Moreau.
Disease English name. dutchelmdisease, elmwiltdisease.
Fungi industry Fungi, Ascomycota Ascomycota, Ascomycetes Ascomycetes, fecal bacteria Branch subclass Sordariomycetidae, long
Ceratocystis head Ophiostomatales, Ceratocystis families Ophiostomataceae, Ceratocystis Ophiostoma.
Elm Fusarium oxysporum For additional information, see Appendix A.
3 PRINCIPLE OF THE METHOD
Based on morphological and biological characteristics of asexual spores and sexual generation, as well as its characteristic symptoms caused by host detection and identification.
4 Instrument
4.1 biological microscope.
4.2 stereo microscope.
4.3 Light biological incubator.
4.4 autoclave.
4.5 Clean Benches.
4.6 electronic balance.
4.7 shaker.
5 Reagents and culture medium
5.1 Reagents
Agar powder, malt extract, Oxoid malt extract, elm branches, glucose, asparagine (L-asparagine), potassium dihydrogen phosphate
(KH2PO4), magnesium sulfate (MgSO4 · 7H2O), zinc sulfate (ZnSO4), ferric chloride (FeCl3), vitamin B1, vitamin B6, Actinobacillus
Cycloheximide, streptomycin, penicillin.
NOTE. actidione as virulent, pay attention to protection.
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