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GB/T 27827-2011: Chemicals -- Test method of in vivo sister chromatid exchange
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27827-2011 | English | 189 |
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Chemicals -- Test method of in vivo sister chromatid exchange
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GB/T 27827-2011
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Basic data | Standard ID | GB/T 27827-2011 (GB/T27827-2011) | | Description (Translated English) | Chemicals -- Test method of in vivo sister chromatid exchange | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 8,889 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-08-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemicals in vivo sister chromatid exchange test method terminology and definitions, test theory, test methods, test data and reports. This standard applies to chemicals in vivo sister chromatid exchange test. | GB/T 27827-2011: Chemicals -- Test method of in vivo sister chromatid exchange---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Test method of in vivo sister chromatid exchange
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemicals in vivo sister chromatid exchange
experiment method
Issued on. 2011-12-30
2012-08-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard and the US Environmental Protection Agency (UnitedStatesEnvironmentalProtectionAgency, USEPA) prevention, pesticides and
Office of Toxic Substances (theOfficeofPrevention, PesticidesandToxicSubstances, OPPTS) of OPPTS870.
5915.1998 "in vivo sister chromatid exchange test" match (in English) of technical content.
This standard configuration and do the following editorial changes.
--- Increasing the range a chapter;
--- The original text of the "Introduction" section of this standard as "Introduction";
--- The original "definition" as part of the content of this standard "2 Terms and Definitions";
--- A unit of measurement unity to our legal units of measurement.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard was drafted. Chinese Center for Disease Prevention and Control of Occupational Health and Poison Control, Occupational Disease Prevention Hospital in Liaoning Province, China Chemical Industry
Technology Development Center, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Qu Bo, Zheng Lin, Li Xuefei, white feather, Wang Xiaobing, Falls Apart.
Introduction
This standard reference USEPAOPPTS870.5915.1998 in vivo sister chromatid exchange test (in English), and the technical content
It is consistent. The test guide is the US Environmental Protection Agency (EPA) prevention, pesticides and toxic substances Prevention Office (theOfficeofPre-
One series of tests guide vention, PesticidesandToxicSubstances, OPPTS) published for the subjects' Pesticides and Toxic
Substance; toxicological data obtained after the submission for review in accordance with EPA regulations. The test guidelines published by OPPTS
Mergers and weigh a plurality of test guidelines [including pollution prevention and toxic substances office (theOfficeofPolutionPreventionand
Toxics, OPPT) guidelines issued by the US specification Encyclopedia I, Chapter R sub-chapter in the guide under the heading 40 (Title40,
ChapterI, SubchapterRoftheCodeofFederalRegulations, CFR), pesticides Planning Office (theOfficeof
PesticidePrograms, OPP), the Organization for Economic Cooperation and Development (theOrganizationforEconomicCooperationandDe-
velopment, OECD) guidelines a coordinated] after the final formation.
Integration of multiple purpose test guidelines to form a single set of guidelines OPPTS test is to test the number of US Environmental Protection Agency reached
According to requirements, so that differences in test methods is reduced to a minimum.
Applicability. To meet the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA) and the Toxic Substances Control Act (TSCA) to
Seeking formulated guidelines.
Background Information. The OPPTS develop guidelines for testing the synchronization reference information includes. OPPT40CFR798.5915 vivo sisters
Chromatid exchange test and OPP84-2 mutagenicity test (F Pesticide Assessment Guidelines section, hazard evaluation; and livestock) US Environmental Protection
Department report 540/09-82-025,1982.
The ability to test for detection of chemicals to increase the replication of chromosomal DNA of two sister chromatid exchange Sister chromatid exchange.
Cultured mammalian cells can be used in vitro tests, also use non-mammalian or mammalian tissue in vivo tests. Most
Using a mammal (e.g. mouse, rat, hamster) lymphocytes and bone marrow cells, human lymphocytes may also be used.
Chemicals in vivo sister chromatid exchange
experiment method
1 Scope
This standard specifies the chemical test methods for in vivo sister chromatid exchange terms and definitions, test principle, test methods, test number
And, according to the report.
This standard applies to chemicals in vivo sister chromatid exchange tests.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Sister chromatid exchange sisterchromatidexchange, SCE
Two single chromatid exchange chromosome replication in cell division. In the mid-phase of the cell cycle can be observed in this post
Change, it may take a DNA double helix of the DNA fragments, transfer and connectivity.
3 Test principle
Animals were given test substance exposure through appropriate means, then give bromo-deoxyuridine (bromodeoxyuridine, BrdU). move
The former was killed with a spindle inhibitor (colchicine or colchicine) treatment, blocking cell division in metaphase. After the animals were euthanized
Remove the subject tissue, the preparation of tissue samples examined, after staining SCE counted under a microscope to analyze.
Test Method 4
4.1 animal selection
4.1.1 germline
Any suitable mammalian germline can be used. There are commonly used rodent mice, rats and hamsters.
4.1.2 Age
Use healthy young adult animals.
4.1.3 Number and sex
Each exposure group and control group, at least 10 animals, five male and a female. Such as the use of single-sex or a different number of animals should be provided appropriate
reason.
4.1.4 Packet
It should be exposed experimental animals were randomly divided into two groups.
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