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GB/T 27822-2011 English PDF

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GB/T 27822-2011: Chemicals -- Test method of sex-linked recessive lethal in Drosophila Melanogaster
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GB/T 27822-2011English209 Add to Cart 3 days [Need to translate] Chemicals -- Test method of sex-linked recessive lethal in Drosophila Melanogaster Valid GB/T 27822-2011

PDF similar to GB/T 27822-2011


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Basic data

Standard ID GB/T 27822-2011 (GB/T27822-2011)
Description (Translated English) Chemicals -- Test method of sex-linked recessive lethal in Drosophila Melanogaster
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A80
Classification of International Standard 13.300; 11.100
Word Count Estimation 9,942
Date of Issue 2011-12-30
Date of Implementation 2012-08-01
Regulation (derived from) Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the Drosophila sex-linked recessive lethal test of terms and definitions, reference materials, try to face the basic principles, test methods, test data and reports. This standard is applicable to the detection of chemicals melanogaster sex- linked recessive lethal test.

GB/T 27822-2011: Chemicals -- Test method of sex-linked recessive lethal in Drosophila Melanogaster


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Chemicals. Test method of sex-linked recessive lethal in Drosophila Melanogaster ICS 13.300; 11.100 A80 National Standards of People's Republic of China Chemicals Drosophila melanogaster sex-linked recessive lethal test methods Issued on. 2011-12-30 2012-08-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given planning. The Standards and Organisation for Economic Co-operation and Development (OECD) chemical testing methods No.477 (1984 Nian) "Drosophila melanogaster sex-linked recessive lethal Test "is consistent (in English) of technical content. The standard structure and made the following editorial changes. --- Increasing the range a chapter; --- The OECD477 description of "reference material" As part of Chapter 3 of this standard; --- The OECD477 original in the "Experiment Operations Overview" As part of this standard 5.1; --- The original OECD477 the "essential information" as part of the standard 5.2; --- Change the unit of measure of legal units of measurement. This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points. This standard was drafted. Chinese Center for Disease Prevention and Control of Occupational Health and Poison Control, Centers for Disease Control and Prevention, Tianjin, China Chemical Technology Development Center. The main drafters of this standard. Ai Wei Wu, Chao-lin, Yan-let, Wang Xiaobing, a Yang, Liu Jing, Liu Baofeng, Zhang. Chemicals Drosophila melanogaster sex-linked recessive lethal test methods

1 Scope

This standard specifies the terms and definitions Drosophila melanogaster sex-linked recessive lethal test, reference materials, test the basic principles, test methods, test Data and reports. This standard applies to detect chemicals Drosophila melanogaster sex-linked recessive lethal test.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Lethal mutation lethalmutation A genetic change in the group, causing the death of carriers when this group of gene expression. 2.2 Recessive mutations recessivemutation Changes in the homozygous or hemizygous condition expressing a genome. 2.3 Sex-linked gene sex-linkedgenes Present in the sex chromosome (X or Y) on the gene, in this method refers only to genes located on the X chromosome. REFERENCE SUBSTANCES Used as a positive control substances. ethyl methanesulfonate (ethylmethanesulphonate), N- nitroso dimethylamine (N-nitroso- dimethylamine).

4 Test the basic principles

Sex-linked recessive lethal test (sex-linkedrecessiveletha, SLRL) the use of Drosophila melanogaster (DrosophilaMelanogaster) check Measuring the rate of mutation in germ insect cell lines, including point mutations and small deletions. This method is a forward mutation analysis method, it is possible Screening of mutation on the X chromosome locus 800, or 80% of all X chromosome gene locus. X chromosome accounting for the entire genome 1/5 [1]. Drosophila melanogaster X chromosome mutations typical phenotypic performance in carrying the mutant gene in a male subject. When hemizygous fatal When the mutation can be inferred through normal heterozygous females breeding two types of male offspring in a class missing. SLRL test use The characteristics of the chromosome and mark the particularity arrangement.

5 Test methods

5.1 Test Operations Overview Wild-type male Drosophila melanogaster exposed after mating with a suitable female Drosophila melanogaster. The female flies mating again and get the same generation male

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