|
US$229.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 27821-2011: Chemicals -- Bacterial DNA damage or repair test
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27821-2011 | English | 229 |
Add to Cart
|
3 days [Need to translate]
|
Chemicals -- Bacterial DNA damage or repair test
| Valid |
GB/T 27821-2011
|
PDF similar to GB/T 27821-2011
Basic data | Standard ID | GB/T 27821-2011 (GB/T27821-2011) | | Description (Translated English) | Chemicals -- Bacterial DNA damage or repair test | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 10,122 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-08-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemical or bacterial DNA damage repair test principle, test methods, test procedures and test data and reports. This standard applies to chemicals bacterial DNA damage or repair tests. | GB/T 27821-2011: Chemicals -- Bacterial DNA damage or repair test---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Bacterial DNA damage or repair test
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemical or bacterial DNA damage repair test methods
Issued on. 2011-12-30
2012-08-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard and the US Environmental Protection Agency (UnitedStatesEnvironmentalProtectionAgency, USEPA) prevention, pesticides and
Office of Toxic Substances (theOfficeofPrevention, PesticidesandToxicSubstances, OPPTS) of OPPTS
870.5500.1998 bacterial DNA (deoxyribonucleic acid) damage or repair test (Healtheffecttestguidelines-Bacterial
DNAdamageorrepairtests) agreement (in English) of technical content.
This standard configuration and do the following editorial changes.
--- The introductory part to the introduction;
--- Increasing the range chapter (see Chapter 1).
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard was drafted. Chinese Center for Disease Prevention and Control of Occupational Health and Poison Control.
The main drafters of this standard. Liuqing Jun, Chao-lin, Lin Zheng, Shi Xiao Yi, Wei Wu Ai.
Introduction
This standard reference USEPAOPPTS870.5500.1998 bacterial DNA (deoxyribonucleic acid) damage or repair test ((English
Version). The guide is the United States Environmental Protection Agency (UnitedStatesEnvironmentalProtectionAgency, USEPA) Prevention, Pesticides
And the Office of Toxic Substances (theOfficeofPrevention, PesticidesandToxicSubstances, OPPTS) developed for evaluation
Pesticides and other toxic substances, one of a series of safety testing guidelines. Is OPPTS integration testing guidelines and requirements of the relevant departments three
And ultimately the formation of three test instructions are based on the relevant departments of the United States Code of Federal Regulations (CodeofFederalRegulations, Title
Pollution prevention and toxic substances office 40), the first chapter of Part R (theOfficeofPolutionPreventionandToxics,
OPPT) developed test guidelines and requirements, published by the US National Intelligence Agency (theNationalTechnicalInformationService,
NTIS) Pesticide Planning Office (theOfficeofPesticidePrograms, OPP) testing guidelines and requirements, and the World Economic Cooperation
And Development Organization (theOrganizationforEconomicCooperationandDevelopment, OECD) testing guidelines published.
Based on the Toxic Substances Control Regulations (theToxicSubstancesControlAct, 15U.SC2601) and the Federal Insecticide, Fungicide
Agents and agents to kill rodents and regulations (theFederalInsecticide, FungicideandRodenticideAct (7U.SC136, et
seq.), in order to meet the requirements of the test data, USEPA sets guidelines to integrate and form a final OPPTS testing guidelines, to reduce
Low differences caused different test procedures.
Chemical or bacterial DNA damage repair test methods
1 Scope
This standard specifies the test principle chemical injury or bacterial DNA repair test, test methods, test procedures and test data and
report.
This standard applies to chemical or bacterial DNA damage repair test.
2 test principle
Bacterial DNA damage or repair test can be used to test DNA damage repair in a healthy bacteria strains and repair defects
Strain test, repair deficient strain of cell death and growth inhibition in different manifestations. The test itself is not used to measure the mutation events, but
Through interaction with the test substance detection detect genetic material of its genotoxicity. By detecting sound repair strains and lack of repair
Trap strains exhibit different growth inhibition test chemicals to repair healthy wild-type strain and repair defects projections
Variant strains of different cell death induced effect. Repair deficient mutant strain is usually one or more regulatory DNA damage repair
Enzyme deficient strain.
This test method can be used to detect whether chemicals can react with cellular DNA leads to inhibition of cell growth or cause death action. Chemistry
Interaction with DNA products can be identified by specific cell repair system. The method of selecting a specific DNA repair genes intact and lack
One pair of strain trap, since DNA repair-deficient strain can not repair DNA damage caused by a chemical, its performance tends to cells
It tends to growth inhibition or death.
Reference substance present methods include, but are not limited to chloramphenicol (chloramphenicol) and methyl methanesulfonate (methylmethanesul-
fonate).
Test Method 3
3.1 Method of selection
Different methods can be used to test the method should use the following two methods.
a) tests carried out on a solid substrate (diffusion test);
b) in the liquid phase of tests conducted on the substrate (suspension test).
3.2 strain selection
3.2.1 Common strain
Should choose coli (EscherichiacolipolA, W3110/p3478) and Bacillus subtilis (Bacillussubtilisrec, H17 /
M45). Alternatively other strains.
3.2.2 Strain preparation and preservation
Strains preservation solution preparation and preservation, growth conditions, identification and verification of the desired phenotype strains and other microorganisms should adopt good test
Test methods, and should be documented.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 27821-2011_English be delivered?Answer: Upon your order, we will start to translate GB/T 27821-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 27821-2011_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 27821-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|