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GB/T 27820-2011: Chemicals -- Test method of in vitro mammalian cells sister chromatid exchange
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| GB/T 27820-2011 | English | 189 |
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Chemicals -- Test method of in vitro mammalian cells sister chromatid exchange
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GB/T 27820-2011
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Basic data | Standard ID | GB/T 27820-2011 (GB/T27820-2011) | | Description (Translated English) | Chemicals -- Test method of in vitro mammalian cells sister chromatid exchange | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 8,867 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-08-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemical sister chromatid exchange in mammalian cells in vitro test method terminology and definitions, test principles, test methods, test data and reports. This standard applies to chemicals sister chromatid exchange in mammalian cells in vitro tests. | GB/T 27820-2011: Chemicals -- Test method of in vitro mammalian cells sister chromatid exchange---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Test method of in vitro mammalian cells sister chromatid exchange
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemicals in vitro mammalian cell staining sisters
Test methods for exchanging monomers
Issued on. 2011-11-30
2012-08-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard with the United Nations Organisation for Economic Co-operation and Development (OECD) Chemicals testing guidelines No.479 (1986) "in vitro mammalian cell
Sister chromatid exchange test "(in English) is consistent technical content.
This standard configuration and do the following editorial changes.
--- Increasing the range a chapter;
--- The original OECD479 the "Introduction" As part of this standard "Introduction";
--- The original OECD479 the "essential information" part of the contents of this standard as "4.1.1";
--- A unit of measurement unity to our legal units of measurement.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard was drafted. Chinese Center for Disease Prevention and Control of Occupational Health and Poison Control, Occupational Disease Prevention Hospital in Liaoning Province, China Chemical Industry
Technology Development Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Qu Bo, Zheng Lin, Li Xuefei, white feather, Yang Ting, Tang Lijun.
Introduction
In vitro sister chromatid exchange (SCE) test is a rapid test replication between two sister chromatids of chromosome DNA from each other
Test Exchange, SCE representatives of sister chromatid homologous sites on DNA replication product exchange. Although the molecular mechanism is not clear
Chu, but speculated that it may involve the exchange of DNA breakage and reconnection of two steps. Detection of SCEs requires some way different dye
Chromophore labeled. As can be incorporated into the brominated deoxyuridine (bromodeoxyuridine, BrdU) in chromosomal DNA replication and two
The method of cell cycle. SCEs can be performed in a mammalian or non-mammalian systems.
Chemicals in vitro mammalian cell staining sisters
Test methods for exchanging monomers
1 Scope
This standard specifies the chemical mammalian cells in vitro sister chromatid exchange test method terminology and definitions, principles of testing, testing
Methods, test data and reports.
This standard applies to chemicals in mammalian cells in vitro sister chromatid exchange tests.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Sister chromatid exchange sisterchromatidexchange, SCE
Two chromosomes exchange monomer cell division of a single copy of the chromosome. In the mid-phase of cell division can be seen in this post
Change, it may take a DNA double helix of the DNA fragments, transfer and connectivity.
3 Test principle
In mammalian cells, and without the addition of exogenous metabolic activation system conditions, containing the test substance and bromo-deoxyuridine (bro-
modeoxyuridine, BrdU) in the culture medium, continuous culture two cell cycles. Join spindle inhibitor (eg colchicine), will be divided
Split cells remain in the gathering metaphase-like stage, the cells were harvested, prepared chromosomes.
Test Method 4
4.1 Preparation
4.1.1 test substance
The test substance should be provided of state, purity, solubility, melting/boiling point, pH value (if applicable), the vapor pressure (if any) and other information.
Should be prepared medium containing the test substance or the test substance is dissolved in a suitable medium prior to cell exposure, the test substance should be freshly prepared.
Final concentration of the media can not significantly affect cell viability, growth rate and the frequency of SCE.
4.1.2 and cell culture methods
It can use primary cultured cells (such as human lymphocytes) or established cell lines (eg Chinese hamster ovary cells or lung cells). Cell Lines
Mycoplasma contamination and should ensure that no stable karyotype.
The use of appropriate media and culture conditions (such as temperature, dish, CO2 concentration and humidity).
4.1.3 metabolic activation
Respectively, in the presence/absence of suitable mammalian metabolic activation system under the conditions of use of the cells exposed to the test substance. Common metabolic activation system
Enzyme inducers through the mammalian liver homogenates and microsomal enzyme system added cofactor preparation of the activation system, as well as feeding primary culture
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