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GB/T 26628.3-2024: Inspection of grain and oils - Standard atlas of stored grain fungi - Part 3: Fusarium
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Inspection of grain and oils - Standard atlas of stored grain fungi - Part 3: Fusarium
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GB/T 26628.3-2024
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Basic data | Standard ID | GB/T 26628.3-2024 (GB/T26628.3-2024) | | Description (Translated English) | Inspection of grain and oils - Standard atlas of stored grain fungi - Part 3: Fusarium | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B04 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 24,287 | | Date of Issue | 2024-10-26 | | Date of Implementation | 2025-05-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 26628.3-2024: Inspection of grain and oils - Standard atlas of stored grain fungi - Part 3: Fusarium
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ICS 65.020.01
CCSB04
National Standard of the People's Republic of China
Standard Atlas of Stored Grain Fungi for Grain and Oil Inspection
Part 3.Fusarium spp.
Part 3.Fusarium
Released on October 26, 2024
Implementation on May 1, 2025
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Inspection Method 1
5 Colony morphology and microscopic morphology of common Fusarium fungi in stored grain and their description 2
Appendix A (Normative) Observation Procedures for Morphological Characteristics and Microscopic Characteristics of Fusarium Fungi in Grains 16
Appendix B (Informative) Culture Media and Reagents 18
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document is Part 3 of GB/T 26628 "Standard Atlas of Stored Grain Fungi for Inspection of Grain and Oils".
Lower part.
--- Part 1.Aspergillus;
--- Part 2.Penicillium;
--- Part 3.Fusarium;
--- Part 4.Other common bacterial genera.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was submitted by the State Administration of Grain and Material Reserves.
This document is under the jurisdiction of the National Technical Committee on Standardization of Cereals and Oils (SAC/TC270).
This document was drafted by. China National Grain and Oils Corporation Chengdu Storage Research Institute Co., Ltd., National Grain and Material Reserves Administration Scientific Research Institute, China National Grain and Oils Corporation Quality Management Co., Ltd.
Inspection Center Co., Ltd., Henan University of Technology.
The main drafters of this document are. Chen Shuai, Qi Zhihui, Tang Fang, Lei Yuqing, Lan Shengbin, Tian Lin, Zhang Haiyang, Chen Liang, Feng Yongjian, Chen Jinying,
Gu Yuxi, Wang Jin, Yang Chao, Li Li, and Fan Yunqian.
introduction
Fungi are one of the important biological factors that cause the deterioration of grain quality. In order to early evaluate and identify fungi and mycotoxins in grain
To better understand the pollution hazards of grain storage and reduce the risk of grain quality and safety, it is necessary to study the types and distribution of fungi in grain.
The standard atlas of stored grain fungi, which was established based on the different morphological characteristics of different fungi species under different growth conditions, can be used to accurately identify the fungi.
Provide auxiliary reference for fungal inspection in stored grain and improve the accuracy and reliability of fungal identification results.
GB/T 26628 "Standard Atlas of Stored Grain Fungi for Inspection of Grain and Oils" aims to specify the inspection requirements for different species of fungi in stored grains and give
The standard atlas and characteristic descriptions of the main fungi in the species are planned to consist of four parts.
--- Part 1.Aspergillus spp.
--- Part 2.Penicillium spp.
--- Part 3.Fusarium spp.
--- Part 4.Other common bacterial genera.
Standard Atlas of Stored Grain Fungi for Grain and Oil Inspection
Part 3.Fusarium spp.
1 Scope
This document defines the terms and definitions of the Fusarium genus in the Standard Atlas of Stored Grain Fungi, describes the test methods, and gives the common Fusarium species found in stored grains.
Bacterial colony morphological characteristics diagram and microscopic morphological characteristics diagram and characteristic description.
This document is intended as an auxiliary reference for the detection of Fusarium spp. in stored grains.
2 Normative references
This document has no normative references.
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Fusarium
Ascomycota, Sordariomycetes, Hypocreales, Nectri-
aceae).
Note 1.The main characteristics of Fusarium are that the aerial hyphae are sparse, and some have no aerial hyphae at all, and the myxospore layer is directly produced by the matrix hyphae, which contains a large number of conidia
Conidia; Most species of microconidia usually have pseudo-capital, less commonly chain, or a combination of pseudo-capital and chain.
On branched or unbranched peduncles, the shapes are various, including oval, pear-shaped, elliptical, oblong, spindle-shaped, lanceolate, sausage-shaped, cylindrical, cone-shaped, etc.
The number of small conidia is usually greater than that of large conidia; large conidia are produced on the short mycelium.
The large conidia have various shapes, such as sickle-shaped, linear, spindle-shaped, lanceolate, columnar, etc.
The apical cells are various in shape, such as short beak, cone, hook, line, column, etc.
Narrow or suddenly contracted; aerial hyphae, stroma, myxospores, sclerotia can be various colors. The matrix can also be dyed into various colors; thick-walled spores are interstitial or apical.
They grow singly or in clusters or nodules, sometimes in large conidial chambers, and are colorless or of various colors, smooth or rough.
Note 2.Toxigenic fungi of the genus Fusarium mainly include Fusarium graminearum, Fusarium moniliforme, Fusarium trilineata, Fusarium oxysporum and Fusarium equisetum, which can produce single
Secondary metabolites include cetosporium compounds, zearalenone, moniliformin and butenolide.
4.Inspection methods
4.1 Observation of colony morphology
The indoor environment should be bright and well-lit, or the observation should be conducted under a light source with an illumination of 450lx±100lx.
Follow Appendix A. For culture medium preparation, see Appendix B.
4.2 Microscopic morphological observation
According to the different objective lens magnifications, adjust the light source intensity of the microscope and the aperture diaphragm of the condenser. The aperture diaphragm of the condenser should be consistent with the objective lens used.
The numerical aperture of the objective lens should be consistent or slightly larger, so that the entire observation field is bright and suitable. The operation of microscopic morphological characteristics observation shall be carried out according to Appendix A.
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