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GB/T 26612-2011: Procedures of Thoroughbred parentage verification with DNA testing techniques
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 26612-2011 | English | 259 |
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Procedures of Thoroughbred parentage verification with DNA testing techniques
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GB/T 26612-2011
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Basic data | Standard ID | GB/T 26612-2011 (GB/T26612-2011) | | Description (Translated English) | Procedures of Thoroughbred parentage verification with DNA testing techniques | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B43 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 11,196 | | Date of Issue | 2011-06-16 | | Date of Implementation | 2011-11-01 | | Quoted Standard | GA/T 383; SN/T 1119; SN/T 1193 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 9 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the Thoroughbred DNA paternity testing methods used, the process, the results and interpretation of the records and so on. This standard applies to paternity Thoroughbred horses. | GB/T 26612-2011: Procedures of Thoroughbred parentage verification with DNA testing techniques---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Procedures of Thoroughbred parentage verification with DNA testing techniques
ICS 65.020.30
B43
National Standards of People's Republic of China
Thoroughbred DNA paternity testing technical regulations
Issued on. 2011-06-16
2011-11-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The Standard Appendix A, Appendix B, Appendix C, Appendix D, Appendix E is an information appendix.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee of animal husbandry.
This standard was drafted. Horse Research Center, China Agricultural University, China Horse Industry Association.
The main drafters of this standard. Zhao Chunjiang, Wu Changxin, only South Korea, Zhang Hao, Wuke Liang, Du Dan, Han Wenpeng.
Thoroughbred DNA paternity testing technical regulations
1 Scope
This standard specifies the Thoroughbred DNA paternity test used methods, procedures, records and interpret the results and the like.
This standard applies to paternity Thoroughbred horses.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GA/T 383 forensic DNA laboratory testing specification
SN/T 1119 imports of animal feed and sheep derived materials in PCR method
SN/T 1193 genetic testing laboratory technical requirements
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Thoroughbred horse Thoroughbred
In line with international Thoroughbred Registration Board (InternationalStudBookCommittee, ISBC) regulations, approved by the members of the ISBC
State Registration Board registered horses.
3.2
Microsatellite DNA paternity testing method parentagetestingwithDNAtechnique
Parent Identification of microsatellite DNA polymorphisms and Mendelian basis.
Principle 4
According flanking primers designed microsatellite DNA, microsatellite DNA loci were amplified, including the International Association of Animal Genetic
Nine microsatellite loci will (InternationalSocietyofAnimalGenetics, ISAG) and ISBC requirements. Judged by gel electrophoresis
Given genotype of the individual. According to Mendelian inheritance, than the individual parent and offspring each microsatellite DNA loci genotype whether
Consistent, so as to achieve the purpose of paternity.
5 Reagents and Equipment
5.1 Reagents
5.1.1 ethanol (ethanol), AR.
5.1.2 isopropyl alcohol (isopropanol), AR.
5.1.3 Sodium hydroxide (NaOH), AR.
5.1.4 disodium edetate (EDTA), AR.
5.1.5 Sodium chloride (NaCl), AR.
5.1.6 sodium dodecyl sulfate (SDS), AR.
5.1.7 Potassium acetate (CH3COOK), AR.
5.1.8 Lithium chloride (LiCl), AR.
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