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GB/T 24863-2010: Technical regulation of farm animals cell culture and cryopreservation
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 24863-2010 | English | 139 |
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Technical regulation of farm animals cell culture and cryopreservation
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GB/T 24863-2010
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Basic data | Standard ID | GB/T 24863-2010 (GB/T24863-2010) | | Description (Translated English) | Technical regulation of farm animals cell culture and cryopreservation | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B40 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 6,631 | | Date of Issue | 2010-06-30 | | Date of Implementation | 2011-01-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2010 No.2 (Total No.157) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method of preservation of animal cells. This standard applies to pigs, cattle, sheep, horses, asses, chicken, ducks, geese and other poultry in vitro cell culture and preservation. | GB/T 24863-2010: Technical regulation of farm animals cell culture and cryopreservation---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical regulation of farm animals cell culture and cryopreservation
ICS 65.020.30
B40
National Standards of People's Republic of China
Animal and in vitro cell
Cryopreservation technical regulations
Issued on. 2010-06-30
2011-01-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee on Animal Husbandry (SAC/TC274) centralized.
This standard was drafted. Beijing Animal Husbandry and Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
The main drafters of this standard. Guan Wei Jun, Ma Yuehui, Lixiang Chen, Li Han, Yan Lei, Zhao Qianjun, lotus palace.
Animal and in vitro cell
Cryopreservation technical regulations
1 Scope
This standard specifies the method to save the livestock body cells.
This standard applies to pigs, cattle, sheep, horses, donkeys, chickens, ducks, geese and other poultry in vitro cell culture and preservation.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
The somatic cells in vitro and in vivo simulated conditions of the living environment in culture, the cells proliferation and retain their structural integrity and functionality
The method of characteristics.
3.2
The cells were cultured in a simulated environment in vivo survival, growth and proliferation of the cell culture method can achieve passage states.
3.3
Cultured cells after cryopreservation fluid mixing by a certain percentage, the cooling rate is set by cooling to liquid nitrogen for long term storage of
process.
4 Workspace conditions
Workspace generally from the preparation room, buffer room, sterile room, cell preservation chamber.
The inter-buffer area should not be less than 3m2, and a locker and ultraviolet light, ultraviolet light intensity is not less than 1.5W/m3. purple
Outside the tube from the ground should not exceed 2.5m, each exposure time was 20min ~ 30min. Minimum standards Aseptic sterile room level should reach
To ten thousand.
5 major instruments and equipment
Clean table, refrigerator, oven blast, high-pressure steam sterilizer, liquid nitrogen biological containers, centrifuges, electronic balance, CO2 incubator,
Ultra-pure water equipment and microscopes.
6 Cleaning and disinfection
6.1 glassware cleaning and disinfection
6.1.1 Soak
Required glassware containing detergent 清水中浸泡 12h.
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