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GB/T 24773-2009 English PDF

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GB/T 24773-2009: Determination of purity for ursolic acid -- High performance liquid chromatographic method
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Basic data

Standard ID GB/T 24773-2009 (GB/T24773-2009)
Description (Translated English) Determination of purity for ursolic acid -- High performance liquid chromatographic method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard G15
Classification of International Standard 71.080.40
Word Count Estimation 6,624
Date of Issue 2009-12-15
Date of Implementation 2010-07-01
Quoted Standard GB/T 6682-2008
Regulation (derived from) National Standard Approval Announcement 2009 No.15 (Total No.155)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the test method for determining the purity of ursolic acid by high performance liquid chromatography. This standard applies from loquat, determination of rosemary extract and other plant products ursolic acid ursolic acid mass fraction.

GB/T 24773-2009: Determination of purity for ursolic acid -- High performance liquid chromatographic method


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Determination of purity for ursolic acid.High performance liquid chromatographic method ICS 71.080.40 G15 National Standards of People's Republic of China Determination of ursolic acid Purity High Performance Liquid Chromatography Posted 2009-12-15 2010-07-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by China Petroleum and Chemical Industry Association. This standard by the National Standardization Technical Committee on Organic Chemistry Chemical Technical Committee (SAC/TC63/SC2) centralized. This standard is drafted by. Yichun. Participated in the drafting of this standard. Beijing-day treasures Biological Technology Co., Ltd. The main drafters of this standard. Li Kaiquan, Ye Wenfeng, had Xiaomin, Zousheng Qin Qian Yuan Hua, Han Xiao, Yu Guoping, Peng Junping. Determination of ursolic acid Purity High Performance Liquid Chromatography

1 Scope

This standard specifies the test method for determining the purity of ursolic acid by high performance liquid chromatography. This standard applies to the determination extracted from leaves, rosemary and other plants ursolic acid products ursolic acid mass fraction.

2 Normative references

The following documents contain provisions which, through reference in this standard, constitute provisions of this standard. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682-2008 analytical laboratory use specifications and test methods (ISO 3696. 1987, MOD) Test Method 3 3.1 Warning Some test procedures prescribed test method can lead to dangerous situations, the operator should take appropriate safety and health practices. 3.2 Method summary By high performance liquid chromatography, under the selected operating conditions, the compound through the column and the separation of impurities in the sample solution target with purple External absorption detector, with external standard, calculate the mass fraction of ursolic acid in the sample. 3.3 Reagents 3.3.1 Water, GB/T 6682-2008, Class 2. through 0.45μm membrane filter and degassed ultrasound. 3.3.2 Methanol (HPLC grade). 3.3.3 ursolic acid standard sample. national certified reference materials, ursolic acid mass fraction ≥99.8%. 3.4 Instrument 3.4.1 HPLC system 3.4.1.1 High pressure pump. no pulse, can maintain the flow 0.1mL/min ~ 10.0mL/min. 3.4.1.2 Loop. 10μL. 3.4.1.3 UV absorption detector. variable wavelength. 3.4.1.4 Data Processing System. chromatography workstation or a data processor. 3.4.2 filtration system A pore diameter of 0.45μm filtration system filters cellulose esters (for pretreatment mobile phase). 3.4.3 Filtration System 0.45μm pore size of the filtration system using a cellulose ester membrane filter (used for sample pretreatment). 3.4.4 Injector Autosampler, or micro-syringe (HPLC-specific). 50μL. 3.5 chromatographic conditions Recommended chromatographic columns and typical operating conditions are shown in Table 1, ursolic acid content determination typical HPLC diagram in Appendix A Figure A. 1, the retention time of the components in Appendix A, Table A. 1. Others can achieve the same degree of separation columns and chromatographic operating conditions are be usable.

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