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GB/T 23814-2009 English PDF

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GB/T 23814-2009: Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods
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Basic data

Standard ID GB/T 23814-2009 (GB/T23814-2009)
Description (Translated English) Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 10,134
Date of Issue 2009-05-27
Date of Implementation 2009-09-01
Regulation (derived from) PRC National Standard Approval Announcement 2009 No.7 (Total No.147)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the qualitative PCR method for detection of kidney beans ingredients lotus seed paste articles. This standard applies to semi-finished and finished products from the lotus seed paste ingredients in the qualitative detection of kidney beans. This standard detection limit was 0. 5 ng of kidney DNA.

GB/T 23814-2009: Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods


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Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods ICS 67.050 X04 National Standards of People's Republic of China Lotus seed products beans ingredient Qualitative PCR Detection Posted 2009-05-27 2009-09-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by China National Institute of Standardization. This standard is drafted by. National Processed Food Quality Supervision and Inspection Center (Guangzhou), Guangzhou Municipal Quality Supervision and Testing Institute, China Shenzhen People's Republic of CIQ. The main drafters of this standard. Cai Jun, Qin Fangfang, Luo Haiying, Deng hung bell, Guoxin Dong, Yu-luan, Blue Fang. Lotus seed products beans ingredient Qualitative PCR Detection

1 Scope

This standard specifies the qualitative PCR method to detect kidney beans ingredient lotus seed paste products. This standard applies to semi-finished and finished products by the lotus seed paste ingredients in beans qualitative detection. The standard detection limit of 0.5ng beans DNA.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) GB/T 19495.1 GMO testing - General requirements and definitions GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 19495.7 GMO detection methods of sampling and sample preparation

3 Terms and Definitions

Terms and definitions GB/T 19495.1 established apply to this standard. 4 anti-pollution measures Measures to prevent cross-contamination detection process carried out according to GB/T 19495.2 provisions.

5 Sampling and sample preparation

Execution method according to GB/T 19495.7 requirements.

6 Determination

6.1 Principle The samples were extracted DNA, gene sequence specific for kidney primers were designed by the PCR technique, the gene-specific amplification beans DNA fragments, according to the results of PCR amplification to determine whether the sample contains beans ingredients. 6.2 Reagents and materials Unless otherwise specified, the reagents used were of analytical grade or biochemical reagents, water as a water according to GB/T 6682 regulations. 6.2.1 primer. primer and product information detecting endogenous gene lotus seed paste and kidney beans specific genes in Table 1. Table 1 Primer Information lotus seed products, the desired foreign gene Detection of gene primer sequences PCR product size / bp Gene nature Scope NNEA0732 Positive. 5'-GTAGAATCGGCACTGGAGGTAG-3 ' Anti-. 5'-CATGGGCTAACCAACTAGACAGC-3 ' 142 lotus seed lotus seed specific gene products AB070627 Positive. 5'-CAGACATTTCATATCCAGGGAGG-3 ' Anti-. 5'-TGAATTGTACGGTGAAGGATGG-3 ' 194 kidney beans, lotus seed paste specific gene products

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