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GB/T 23788-2009: Determination of soybean isoflavone in health-care food -- High-performance liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 23788-2009 | English | 189 |
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Determination of soybean isoflavone in health-care food -- High-performance liquid chromatography
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GB/T 23788-2009
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Basic data | Standard ID | GB/T 23788-2009 (GB/T23788-2009) | | Description (Translated English) | Determination of soybean isoflavone in health-care food -- High-performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 8,889 | | Date of Issue | 2009-05-18 | | Date of Implementation | 2009-12-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | PRC National Standard Approval Announcement 2009 No.6 (Total No.146) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for determination of soy isoflavones in health foods. This standard applies to soy isoflavones as the main functional components of health food (tablets, capsule, oral, drinks), can also be used to determine the health food raw soy isoflavone content. The detection limit of this standard: solid, semi- solid samples daidzein, daidzin, Genistin, daidzein, daidzein and genistein component detection limits were 5mg/kg, daidzein liquid samples, soybean rehmaionoside, genistin, daidzein, detection daidzein and genistein component limits are 0. 2mg/L. | GB/T 23788-2009: Determination of soybean isoflavone in health-care food -- High-performance liquid chromatography
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Determination of soybean isoflavine in health-care food. High-performance liquid chromatography
ICS 67.050
X04
National Standards of People's Republic of China
Determination of isoflavones in health food
High Performance Liquid Chromatography
Posted 2009-05-18
2009-12-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is an informative annex.
This standard by the National Standardization Technical Committee special dietary and centralized.
This standard was drafted. Chaoyang District, product quality supervision, inspection, Red Bull Vitamin Drink Co., Ltd., China Food Fermentation Engineering
Industry Research Institute.
The main drafters of this standard. Cui Yan, Xing Xiaohui, Zhang Ligang, the top of its bell, Zhang Shuling, Lang Tao Su Wenying, Wuzhen Jun, Qiu Kai.
Determination of isoflavones in health food
High Performance Liquid Chromatography
1 Scope
This standard specifies the method for determination of soy isoflavones in health food.
This standard applies to soy isoflavones as the main function of health food products (tablets, capsules, oral liquid, beverage), can also be used to protect
Determination of isoflavone content of soy-food raw materials.
The detection limit of this standard. solid, semi-solid samples daidzein, daidzin, genistin, daidzein, genistein and daidzein component
The detection limits were 5mg/kg; daidzin liquid sample, daidzin, genistin, daidzein, genistein and daidzein components
The detection limits were 0.2mg/L.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Daidzein, daidzin, genistin, daidzein, genistein and daidzein, collectively, flavonoids. Formula. Soybean
Glycosides. C21H20O9, daidzin. C22H22O10, genistin. C21H20O10, daidzein. C15H10O4, daidzein. C16H12O5, dyes
Lignin. C15H10O5.
4 Method summary
Sample preparation, extraction, filtered and analyzed by high performance liquid chromatography (C18 column separation), based on the retention time qualitative, with external standard.
5 Reagents and materials
Used in this standard in line with GB/T 6682 provided a water. Subject to use only analytical grade reagents.
5.1 acetonitrile. chromatography.
5.2 methanol. pure class distinctions.
5.3 to 80% methanol. methanol 80mL, add water, 20mL, and mix.
5.4 phosphoric acid.
5.5 phosphoric acid aqueous solution. pH was adjusted to 3.0 with phosphoric acid, through 0.45μm membrane filter.
5.6 DMSO. chromatography.
5.7 50% dimethyl sulfoxide solution. take dimethylsulfoxide 50mL, add water, 50mL, and mix.
5.8 soy isoflavones stock standard solution. Weigh soybean glycosides (daidzin), daidzin (glycitin) and genistein (genistin), soybean
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