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GB/T 23215-2008 English PDF

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GB/T 23215-2008: Determination of paralytic shellfish poison in shellfish -- HPLC-fluorescence detection method
Status: Obsolete
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PDF similar to GB/T 23215-2008


Standard similar to GB/T 23215-2008

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Basic data

Standard ID GB/T 23215-2008 (GB/T23215-2008)
Description (Translated English) Determination of paralytic shellfish poison in shellfish -- HPLC-fluorescence detection method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 10,169
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the paralytic shellfish toxins in shellfish by high performance liquid chromatographic method. This standard applies to the determination of paralytic shellfish toxins in shellfish. This standard method detection limit: GTX4 as 16. 7��g/kg, GTX1 is 50. 7��g/kg, dcGTX3 as 4. 8��g/kg, B1 is 31. 9��g/kg, dcGTX2 as 17. 3��g/kg, GTX3 as 6. 5��g/kg, GTX2 to 19. 6��g/kg, neoSTX as 15. 7��g/kg, dcSTX as 12. 5��g/kg, STX is 14. 5��g/kg, combined total of paralytic shellfish toxins (STXeq) is 125��g/kg.

GB/T 23215-2008: Determination of paralytic shellfish poison in shellfish -- HPLC-fluorescence detection method


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Determination of paralytic shellfish poison in shellfish. HPLC-fluorescence detection method ICS 67.050 X04 National Standards of People's Republic of China Determination of shellfish variety of paralytic shellfish toxin levels Liquid chromatography - fluorescence detection Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard is drafted by. People's Republic of China Qinhuangdao Exit Inspection and Quarantine, Shenzhen People's Republic of China Entry-Exit Inspection Quarantine Bureau, People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. Ouyang Shan, Xieli Qi, Zhu Yulan, Yuezhen Feng, Chen Jincan, Pei Jin, Song Wenbin, Pang Guofang. Determination of shellfish variety of paralytic shellfish toxin levels Liquid chromatography - fluorescence detection

1 Scope

This standard specifies the paralytic shellfish toxins in shellfish by high performance liquid chromatographic method. This standard applies to the determination of paralytic shellfish toxins in shellfish. The standard method detection limit. GTX4 to 16.7μg/kg; GTX1 is 50.7μg/kg; dcGTX3 of 4.8μg/kg; B1 is 31.9μg/kg; dcGTX2 of 17.3μg/kg; GTX3 of 6.5μg/kg; GTX2 of 19.6μg/kg; neoSTX of 15.7μg/kg; dcSTX to 12.5μg/kg; STX is 14.5μg/kg; combined total of paralytic shellfish toxins (STXeq) is 125μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Sample of paralytic shellfish toxins with 0.1mol/L hydrochloric acid extraction, after centrifugation, the supernatant was C18 solid-phase extraction (SPE), then by Zeolite molecular weight over 10,000 ultrafiltration tubes filtered and the filtrate was separated by high performance liquid chromatography, after post-column reaction online, Fluorescence detection, external standard.

4 Reagents and materials

Unless otherwise specified, the reagents used were of analytical grade. 4.1 Water. GB/T 6682, level. 4.2 acetonitrile. chromatography. 4.3 acetic anhydride. 4.4 hydrochloric acid. 36% to 38% (by mass). 4.5 heptane sulfonate. 4.6 phosphoric acid. 85% (mass fraction). 4.7 periodic acid dihydrate. 4.8 dipotassium hydrogen. 4.9 potassium hydroxide. 4.10 ammonia. 4.11 100mmol/L sodium heptane solution. Weigh 20.2g heptane sulfonate (4.5), dissolved in water and dilute to 1000mL.

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