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GB/T 22996-2008 English PDF

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GB/T 22996-2008: Determination of ginsenosides in ginseng -- LC-UV method
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Basic data

Standard ID GB/T 22996-2008 (GB/T22996-2008)
Description (Translated English) Determination of ginsenosides in ginseng -- LC-UV method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 9,979
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.26 (Total No.139)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the ginsenosides in ginseng (ginsenosides) Re, Rb2 content of liquid chromatography with UV detection methods, Rc, Rf, , Rb1 Rg1. This standard applies to sun-dried ginseng ginsenosides Re, Rg1, Rf, Rb1, Rc, determination Rb2 content. This standard method detection limit: ginsenosides Re, Rg1, Rb1, Rc, Rb2 are 50mg/kg, ginsenosides Rf is 25mg/kg.

GB/T 22996-2008: Determination of ginsenosides in ginseng -- LC-UV method

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Determination of ginsenosides in ginseng. LC-UV method ICS 67.050 X04 National Standards of People's Republic of China Determination of ginsenosides in ginseng variety of content LC - UV detection method Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine, People's Republic of China Liaoning Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Song Wenbin, Li Jun, Dongzhen Lin, Sun Yuling, Mike Sui, Lijia Chang, in a vast, on behalf of the British Cheng, Li Shu, Pang Guofang. Determination of ginsenosides in ginseng variety of content LC - UV detection method

1 Scope

This standard specifies the ginsenosides in ginseng (ginsenosides) Re, Rg1, Rf, Rb1, Rc, Rb2 content Liquid Chromatography - ultraviolet detector method. This standard is applicable to the determination of ginseng ginsenosides Re, Rg1, Rf, Rb1, Rc, Rb2 content in raw sun. The standard method detection limit. ginsenosides Re, Rg1, Rb1, Rc, Rb2 were 50mg/kg, ginsenoside Rf is 25mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard. However, according to research encourages the parties to the agreement Study whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1- 2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 By rapid solvent extraction (ASE) at high temperature and pressure conditions so that complete and total ginsenosides extracted into methanol, concentrated by Reduction, volume, liquid chromatography, external standard.

4 Reagents and materials

Water as a water GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 acetonitrile. chromatography. 4.3 sea sand. pure chemistry, particle size. 0.65mm ~ 0.85mm. 4.4 ginsenosides Standards. Re, Rg1, Rf, Rb1, Rc, Rb2 purity greater than 99%. 4.5 ginsenosides standard solution 4.5.1 six kinds of ginsenosides Re, Rg1, Rf, Rb1, Rc, Rb2 standard stock solution. 1.0mg/mL. Analytical balance accurately weighed amount The substance (4.4), respectively, with methanol (4.1) formulated into standard stock solution 1.0mg/mL of. At 2 ℃ ~ 4 ℃ in dark stock solution save. 4.5.2 six kinds of ginsenosides working standard solutions. The linear range for each ginsenosides sensitivity and instrument, the appropriate amount of six Kind of Ginsenosides stock standard solution (4.5.1), prepared in methanol and mixed standard working solution, stored in the dark at 2 ℃ ~ 4 ℃. Note. taking said quality reference material is corrected in accordance with the quality of purity.

5 Instruments

5.1 liquid chromatograph. equipped with a UV detector.

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