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GB/T 22981-2008 English PDF

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GB/T 22981-2008: Determination of bacitracin residues in milk and milk powder -- LC-MS-MS method
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GB/T 22981-2008English209 Add to Cart 3 days [Need to translate] Determination of bacitracin residues in milk and milk powder -- LC-MS-MS method Valid GB/T 22981-2008

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Basic data

Standard ID GB/T 22981-2008 (GB/T22981-2008)
Description (Translated English) Determination of bacitracin residues in milk and milk powder -- LC-MS-MS method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 9,984
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.26 (Total No.139)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the milk and milk powder bacitracin residues liquid chromatography-tandem mass spectrometry. This standard applies to milk and milk powder-Determination of residues of bacitracin and confirmation. This standard method detection limit: Milk 50. 0��g/kg, milk 250��g/kg.

GB/T 22981-2008: Determination of bacitracin residues in milk and milk powder -- LC-MS-MS method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of bacitracin residues in milk and milk powder. LC-MS-MS method ICS 67.050 X04 National Standards of People's Republic of China Determination of milk and milk powder bacitracin residues Liquid chromatography - tandem mass spectrometry Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Exit Inspection and Quarantine Qinhuangdao, Hebei People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. segment Wenzhong, Mayu Song, Guo Chunhai, Wang Fengchi, Gena, Yi Lianfeng, Doucai Yun, Lv Hongying, Pang Guofang. Determination of milk and milk powder bacitracin residues Liquid chromatography - tandem mass spectrometry

1 Scope

This standard specifies the milk and milk powder bacitracin residues in liquid chromatography - tandem mass spectrometry. This standard applies to milk and milk powder bacitracin residues measured and confirmed. The standard method detection limit. milk 50.0μg/kg; milk 250μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Bacitracin residues in the sample with acetonitrile - methanol extract a sample of bacitracin residues, liquid chromatography - tandem mass spectrometer, external standard method Quantified.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 acetonitrile. chromatography. 4.3 formic acid. Chromatographic pure. 4.4 Acetonitrile - Methanol (1 + 2). Measure 200mL acetonitrile (4.2) and 100mL methanol (4.1) were mixed. 4.5 0.1% formic acid solution. Pipette 1mL acid (4.3) in 1L of water containing about 800mL flask with water to volume and mix. 4.6 0.1% formic acid in methanol solution. Pipette 1mL acid in methanol containing about 800mL 1L flask with methanol to volume And mix. 4.7 bacitracin standard substance. bacitracin A (CAS. 1505-87-4) purity not less than 92.0%, the following criteria are bacitracin, Bacitracin A meter. Standard stock solution 4.8 bacitracin. Accurately weigh an adequate amount of bacitracin A (4.7) in 50mL volumetric flask, with 0.1% formic acid in methanol Preparation of a concentration of 0.1mg/mL of the standard stock solution. The solution was stored at 4 ℃. 4.9 bacitracin standard solution. Pipette bacitracin standard stock solution (4.8) 1.0mL to 10mL volumetric flask, with 0.1% formic acid Methanol solution volume, formulated at a concentration of 10.0μg/mL standard working solution. The solution was stored at 4 ℃. 4.10 bacitracin matrix standard solution. draw the right amount of bacitracin standard solution (4.9) as required, with a blank sample extract Diluted to the appropriate concentration of the matrix standard working solution, prepared before use.

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