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GB/T 22980-2008 English PDF

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GB/T 22980-2008: Determination of flunixin residue in bovine milk and milk powder -- LC-UV method
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GB/T 22980-2008English209 Add to Cart 3 days [Need to translate] Determination of flunixin residue in bovine milk and milk powder -- LC-UV method Valid GB/T 22980-2008

PDF similar to GB/T 22980-2008


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Basic data

Standard ID GB/T 22980-2008 (GB/T22980-2008)
Description (Translated English) Determination of flunixin residue in bovine milk and milk powder -- LC-UV method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 9,991
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.26 (Total No.139)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the raw milk and pure milk of flunixin (flunixin) residual liquid chromatography-UV detection methods. This standard applies to the determination of pure raw milk and milk powder flunixin residues. This standard method detection limit: raw milk is 5. 0��g/L, pure milk of 40��g/kg.

GB/T 22980-2008: Determination of flunixin residue in bovine milk and milk powder -- LC-UV method

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Determination of flunixin residue in bovine milk and milk powder. LC-UV method ICS 67.050 X04 National Standards of People's Republic of China Determination of milk and milk powder flunixin residues LC - UV detection method Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine, People's Republic of China Liaoning Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Song Wenbin, Li Jun, Li Yichen, Clue red, in a vast, Li Bai Ge, Yu Jie, Li Ye, Pang Guofang. Determination of milk and milk powder flunixin residues LC - UV detection method

1 Scope

This standard specifies the pure raw milk and milk powder flunixin (flunixin) residues LC - UV detection method. This standard applies to the determination of pure raw milk and milk powder flunixin residues. The detection limit of this standard. raw milk was 5.0μg/L, pure milk of 40μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard. However, according to research encourages the parties to the agreement Study whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1- 2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Samples were digested with acetonitrile under acidic conditions extracted by n-hexane and solid-phase extraction (SPE), the configuration of the high performance liquid UV detector Determination phase chromatography, external standard.

4 Reagents and materials

Unless otherwise indicated, all reagents were of analytical grade. Water as a water GB/T 6682 regulations. 4.1 formic acid. 4.2 glacial acetic acid. 4.3 β- glucuronidase (β-glucuronidase) 15000U/mL. β- glucuronidase (600000U/g), accurately weighed 0.0500gβ- glucuronidase, add 2mL phosphate (PBS) buffer solution was dissolved with 0.45μm nylon membrane filter. use Before now formulated. 4.4 glucose. 4.5 concentrated hydrochloric acid. 4.6 Mobile phase A. ammonium acetate 0.05mol/L, pH5.0 (accurately weighed 3.90g ammonium acetate, dissolved in water and adjusted to formic acid pH 5.0, transferred to a 1000mL volumetric flask, through 0.45μm filter prior to use). 4.7 Mobile phase B. methanol, chromatography. 4.8 Mobile phase C. acetonitrile chromatography. 4.9 mobile phase mixture. 40mL of the mobile phase B, 30mL and 30mL mobile phase C mobile phase A are mixed. 4.10 phosphate (PBS) buffer solution. Weigh 0.8g NaCl, 0.02g KCl, 0.02g potassium dihydrogen phosphate, 0.115g hydrogen phosphate Disodium glucose and 0.02g in 250mL beaker add approximately 90mL of water to dissolve, adjust pH6.0 with 0.1mol/L phosphoric acid, transferred to 100mL volumetric flask and dilute to volume.

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