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GB/T 22969-2008 English PDF

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GB/T 22969-2008: Determination of streptomycin, dihydrostreptomycin and kanamycin residues in milk and milk powder -- LC-MS-MS method
Status: Valid
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GB/T 22969-2008English229 Add to Cart 3 days [Need to translate] Determination of streptomycin, dihydrostreptomycin and kanamycin residues in milk and milk powder -- LC-MS-MS method Valid GB/T 22969-2008

PDF similar to GB/T 22969-2008


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Basic data

Standard ID GB/T 22969-2008 (GB/T22969-2008)
Description (Translated English) Determination of streptomycin, dihydrostreptomycin and kanamycin residues in milk and milk powder -- LC-MS-MS method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 10,131
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.26 (Total No.139)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the milk, milk streptomycin, dihydrotestosterone streptomycin and kanamycin residues in liquid chromatography-tandem mass spectrometry. This standard applies to milk, dihydrotestosterone streptomycin and kanamycin determined residues, milk streptomycin. This standard method detection limit: milk streptomycin, dihydrotestosterone streptomycin and kanamycin detection limits were 10. 0��g/kg, milk powder streptomycin, dihydrotestosterone streptomycin and kanamycin the detection limits are prime 80. 0��g/kg.

GB/T 22969-2008: Determination of streptomycin, dihydrostreptomycin and kanamycin residues in milk and milk powder -- LC-MS-MS method


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Determination of streptomycin, dihydrostreptomycin and kanamycin residues in milk and milk powder. LC-MS-MS method ICS 67.050 X04 National Standards of People's Republic of China Milk and milk streptomycin, dihydrostreptomycin And kanamycin residues Determination Liquid chromatography - tandem mass spectrometry Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine. The main drafters of this standard. FEDERATION Zhangshou Jun, Zhang Jinjie, Caoyan Zhong, Cao Yaping, Yang Zhiwei, Pang Guofang. Milk and milk streptomycin, dihydrostreptomycin And kanamycin residues Determination Liquid chromatography - tandem mass spectrometry

1 Scope

This standard specifies the milk, milk streptomycin, dihydrostreptomycin and kanamycin residues in liquid chromatography - tandem mass spectrometry. This standard applies to milk, milk streptomycin, dihydrostreptomycin and determination of kanamycin residues. The standard method detection limit. milk streptomycin, dihydrostreptomycin and kanamycin detection limits were 10.0μg/kg, milk powder chain Neomycin, dihydrostreptomycin and kanamycin detection limits were 80.0μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1- 2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Milk or milk samples with antibiotic phosphoric acid extraction, TCA protein precipitation, solid phase extraction column with acid type and acid type Purification. Liquid chromatography - tandem mass spectrometry (ESI +) detection, external standard.

4 Reagents and materials

Unless otherwise indicated, the reagents are pure class distinctions. 4.1 Water. GB/T 6682, level. 4.2 Methanol. chromatographically pure. 4.3 acetonitrile. chromatography. 4.4 formic acid. 4.5 concentrated phosphoric acid. 4.6 dipotassium hydrogen phosphate (K2HPO4). 4.7 TCA (C2HCl3O2). 4.8 heptane sulfonate (C7H15NaO3S · H2O). 4.9 5% phosphoric acid solution (1 + 19). Take 50mL concentrated phosphoric acid (4.5), water volume to 1L. 4.10 0.2mol/L phosphate buffer solution. pH = 8.5. He said dissolved in water taken 34.8g potassium phosphate dibasic (4.6), set the volume to 1L, with Sodium hydroxide solution to adjust pH = 8.5. 4.11 trichloroacetic acid solution. 50% (mass fraction). Take 20g trichloroacetic acid (4.7), water volume to 20mL. 4.12 0.01mol/L sodium heptane solution. Weigh 2.20g of heptane sulfonate (4.8), dissolved in water, dilute to 1L.

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