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GB/T 22961-2008 English PDF

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GB/T 22961-2008: Determination of oxytetracycline, tetracycline, chlortetracycline, doxycycline residues in fugu and eel -- HPLC-UV method
Status: Obsolete
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GB/T 22961-2008English359 Add to Cart 3 days [Need to translate] Determination of oxytetracycline, tetracycline, chlortetracycline, doxycycline residues in fugu and eel -- HPLC-UV method Obsolete GB/T 22961-2008

PDF similar to GB/T 22961-2008


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Basic data

Standard ID GB/T 22961-2008 (GB/T22961-2008)
Description (Translated English) Determination of oxytetracycline, tetracycline, chlortetracycline, doxycycline residues in fugu and eel -- HPLC-UV method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 9,924
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Adopted Standard AOAC 995.09, MOD
Regulation (derived from) National Standard Approval Announcement 2008 No.26 (Total No.139)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the puffer fish, tetracycline, , chlortetracycline, eel oxytetracycline and doxycycline residues liquid chromatography-UV detection methods. This standard applies to puffer fish, Eel oxytetracycline, tetracycline, chlortetracycline, determination of doxycycline residues. This standard method detection limits were 0. 010mg/kg.

GB/T 22961-2008: Determination of oxytetracycline, tetracycline, chlortetracycline, doxycycline residues in fugu and eel -- HPLC-UV method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of oxytetracycline, tetracycline, chlortetracycline, doxycycline residues in fugu and eel. HPLC-UV method ICS 67.050 X04 National Standards of People's Republic of China Puffer fish, eel oxytetracycline, tetracycline, Chlortetracycline, doxycycline residues determination LC - UV detection method Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The revised standard 995.09 "edible animal tissues oxytetracycline, tetracycline, chlortetracycline residues were measured using standard AOAC Phase chromatography. " The main technical differences compared to the standard and AOAC standards are as follows. --- C18 solid phase extraction column to OasisHLB SPE. --- Eluent from methanol to ethyl acetate. --- Increased acid cation exchange column purification. --- Increased determination varieties. The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision, Inspection and Quarantine. This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine. The main drafters of this standard. Caoyan Zhong, Shiyu Qiu, Jia light group, Gu Xiaopeng, Pang Guofang. Puffer fish, eel oxytetracycline, tetracycline, Chlortetracycline, doxycycline residues determination LC - UV detection method

1 Scope

This standard specifies the puffer fish, eel oxytetracycline, tetracycline, chlortetracycline, and doxycycline residues LC - UV detection method. This standard applies to fish, eel oxytetracycline, tetracycline, chlortetracycline, doxycycline residues determination puffer. The standard method detection limits were 0.010mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Puffer fish buffer solution was extracted with 0.1mol/LNa2EDTA-Mclvaine (pH = 4.0 ± 0.05), eel tetracycline antibiotic Hormone residues, extract after centrifugation, the supernatant was OasisHLB1) or equivalent solid phase extraction column and a carboxylic acid type cation exchange column purification, fluid Phase Chromatography - ultraviolet detector measurement, external standard. 1) OasisHLB solid phase extraction columns are Waters company's product trade name, this information is given for the convenience of users of this standard, not the table Endorsement of the product shown. If other equivalent product has the same effect, you can use these equivalent products.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade. 4.1 Water. GB/T 6682, level. 4.2 Methanol. chromatographically pure. 4.3 acetonitrile. chromatography. 4.4 ethyl acetate. chromatography. 4.5 hexane. chromatographically pure. 4.6 citric acid (C6H8O7 · H2O). 4.7 dibasic sodium phosphate. 4.8 Disodium ethylenediaminetetraacetate (Na2EDTA · 2H2O). 4.9 oxalic acid. 4.10 formic acid. pure class distinctions.

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