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GB/T 22944-2008 English PDF

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GB/T 22944-2008: Determination of clenbuterol residues in honey -- LC-MS-MS method
Status: Valid
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GB/T 22944-2008English189 Add to Cart 3 days [Need to translate] Determination of clenbuterol residues in honey -- LC-MS-MS method Valid GB/T 22944-2008

PDF similar to GB/T 22944-2008


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Basic data

Standard ID GB/T 22944-2008 (GB/T22944-2008)
Description (Translated English) Determination of clenbuterol residues in honey -- LC-MS-MS method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 8,872
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.26 (Total No.139)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the amount of clenbuterol residues in honey by liquid chromatography-tandem mass spectrometry. This standard applies to the determination of clenbuterol residues in honey. This standard method detection limit of 1 ��g/kg.

GB/T 22944-2008: Determination of clenbuterol residues in honey -- LC-MS-MS method

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Determination of clenbuterol residues in honey. LC-MS-MS method ICS 67.050 X04 National Standards of People's Republic of China Honey Determination of clenbuterol residues Liquid chromatography - tandem mass spectrometry Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision, Inspection and Quarantine. This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine. The main drafters of this standard. Caoyan Zhong, Shiyu Qiu, Jia light group, Yao Zhihui, FEDERATION Pang Guofang. Honey Determination of clenbuterol residues Liquid chromatography - tandem mass spectrometry

1 Scope

This standard specifies the amount of clenbuterol residues in honey by liquid chromatography - tandem mass spectrometry. This standard applies to the determination of clenbuterol residues in honey. The standard method detection limit of 1μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Clenbuterol residues in honey with sodium acetate buffer solution (pH = 5.0) extracted, filtered, dried OasisHLB1) or equivalent solid phase Extraction column, eluted with a solution of ammonia and methanol evaporated and the residue was dissolved in methanol and 0.1% formic acid solution, over 0.2μm membrane, the sample solution For liquid chromatography - tandem mass spectrometer, internal standard.

4 Reagents and materials

1) OasisHLB solid phase extraction columns are Waters company's product trade name, this information is given for the convenience of users of this standard, not the table Endorsement of the product shown. If other equivalent product has the same effect, you can use these equivalent products. 4.1 Water. GB/T 6682, level. 4.2 Methanol. chromatographically pure. 4.3 acetonitrile. chromatography. 4.4 Sodium acetate. pure class distinctions. 4.5 glacial acetic acid. pure class distinctions. 4.6 ammonia. AR. 4.7 formic acid. pure class distinctions. 4.8 0.2mol/L sodium acetate buffer solution. Weigh 16.4g of sodium acetate (4.4), into 1000mL beaker, add 800mL water soluble Solution, with glacial acetic acid (4.5) was adjusted to a pH of 5.0, and then water volume to 1000mL. 4.9 ammonia - methanol solution (1 + 19). lessons 5mL ammonia (4.6) and 95mL of methanol (4.2) and mix well. 4.10 in methanol (3 + 7). Measure 30mL of methanol and 70mL of water were mixed uniformly. 4.11 0.1% formic acid solution. lessons 1.0mL of formic acid (4.7), diluted with water to 1000mL. 4.12 methanol and 0.1% formic acid (1 + 19). Amount of 5mL of methanol and 95mL0.1% formic acid solution (4.11) and mixed. 4.13 clenbuterol standard substance (CAS37148-27-9). purity ≥95%.

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