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GB/T 22329-2008: Diagnostic techniques for bovine hypodermosis
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| GB/T 22329-2008 | English | 209 |
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Diagnostic techniques for bovine hypodermosis
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GB/T 22329-2008
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Basic data | Standard ID | GB/T 22329-2008 (GB/T22329-2008) | | Description (Translated English) | Diagnostic techniques for bovine hypodermosis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 9,941 | | Date of Issue | 2008-08-22 | | Date of Implementation | 2008-12-01 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.14 (Total No.127) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the leather myiasis diagnostic technology requirements, including clinical diagnostics, animal necropsy and enzyme-linked immunosorbent assay. This standard applies to leather myiasis diagnosis. | GB/T 22329-2008: Diagnostic techniques for bovine hypodermosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for bovine hypodermosis
ICS 11.220
B41
National Standards of People's Republic of China
Hypoderma bovis disease diagnostic technology
Posted 2008-08-22
2008-12-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The Standard Appendix A, Appendix B and Appendix C are normative appendix.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee on Animal Epidemic Prevention.
This standard was drafted. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
The main drafters of this standard. Yin Hong, LUO Jian-xun, Guan Gui-quan.
Hypoderma bovis disease diagnostic technology
1 Scope
This standard specifies requirements for leather myiasis (bovinehypodermosis) diagnostic techniques, including clinical diagnostics, animal necropsy and enzyme-linked
Immunosorbent assay (ELISA).
This standard applies to Hypoderma bovis disease diagnosis.
2 clinical diagnosis and autopsy examination
2.1 clinical diagnosis
Bovine Hypoderma infection, generally do not exhibit obvious clinical symptoms, but severe infection, young animals may show weight loss, slow growth, anemia, mother
Cow milk production decline, draft animals causative ability to reduce other symptoms. When the leather fly larvae bore into the brain, it can cause neurological symptoms, such as a backward movement,
Suddenly fell to the ground, paralysis or syncope, severe cases can cause death. In addition, since the third stage larvae perforations formed on the skin, caused by bacterial infection
Suppuration, fistula formation, often pus and serous outflow fistula healed, scar formation. However, these symptoms only as a reference for diagnosing
Standard, can not serve as a basis for diagnosis. Only when the tumor was found swollen and subcutaneous cellulitis-like ridges on the back of the cow, and the third stage larvae can be extruded
When, before being diagnosed.
2.2 necropsy examination
When the affected areas to carry out epidemiological investigation, often using this method. Focus on examination of parts of the esophageal mucosa, subcutaneous, rumen serosa,
Omentum, esophagus and other parts of serous membrane, such as found in the first phase, the second phase or third larval stage any stage can be confirmed.
2.2.1 The first form of larvae
Milky white, long 3.5mm ~ 12mm, width 0.75mm ~ 2mm, slightly pointed tip.
2.2.2 The second form of larvae
Pale yellow white, length 11mm ~ 15mm, width 3mm ~ 6mm. Ventral micro Long, the back of the flat. Exterior mouth hook only see two brown
Dots, separated from each other.
2.2.3 The third stage larvae form
Body length 19mm ~ 25mm, width 8mm ~ 11mm, the aspect ratio (2.3 to 2.1) .1 back flat, belly bulge, side warts
Like projection, color varies maturity varies from yellowish white to light brown, brown and even black body with section 11 (section 3 chest, abdomen
Section 8), pseudo-head with a sensor head, separated from each other. Mouth hook degenerate into black dots.
3.1 Test Material
3.1.1 Antigen
Preparation see Appendix A.
3.1.2 positive serum.
Since leather myiasis endemic areas jugular vein tumor package acquisition back with bovine blood serum was separated by foreign commercial kits tested positive
, Press 3.1 (3 volumes of serum .1 volume glycerol) Glycerin, mix, packed in 1.5mL tubes, -20 ℃ stored for use.
3.1.3 standard negative serum
Since no leather myiasis popular southern province collected via the jugular vein of cattle blood serum was separated by foreign commercial kits for the detection of female
After sex, according to 3.1 (3 volumes of serum .1 volume glycerol) Glycerin, mix, packed in 1.5mL tubes, -20 ℃ stored for use.
3.1.4 enzyme-labeled secondary antibody
Horseradish peroxidase-labeled rabbit anti-bovine IgG, from biochemical reagents purchased.
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