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GB/T 22101.4-2009: Technical specification for evaluating resistance of cotton to diseases and insect pests -- Part 4: Fusarium wilt
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 22101.4-2009 | English | 189 |
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Technical specification for evaluating resistance of cotton to diseases and insect pests -- Part 4: Fusarium wilt
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GB/T 22101.4-2009
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Basic data | Standard ID | GB/T 22101.4-2009 (GB/T22101.4-2009) | | Description (Translated English) | Technical specification for evaluating resistance of cotton to diseases and insect pests -- Part 4: Fusarium wilt | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 8,859 | | Date of Issue | 2009-10-30 | | Date of Implementation | 2009-12-01 | | Regulation (derived from) | National Standard Approval Announcement 2009 No.12 (Total No.152) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard applies to cotton resistance to Fusarium wilt resistance Identification and assessment. | GB/T 22101.4-2009: Technical specification for evaluating resistance of cotton to diseases and insect pests -- Part 4: Fusarium wilt
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical specification for evaluating resistance of cotton to diseases and insect pests Part 4. Fusarium wilt
ICS 65.020
B16
National Standards of People's Republic of China
Cotton pest resistance evaluation of technical specifications
Part 4. blight
Posted 2009-10-30
2009-12-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 22101 "cotton pest resistance evaluation of technical specifications" is divided into five parts.
--- Part 1. cotton bollworm;
--- Part 2. aphids;
--- Part 3. pink bollworm;
--- Part 4. wilt;
--- Part 5. Verticillium wilt.
This part of GB /Part 4 T 22101 of.
Appendix B to this part of the normative Appendix Appendix A is an information appendix.
This part of the People's Republic of China Ministry of Agriculture and focal points.
This section drafted by. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, the National Agricultural Technology Extension and Service Center.
The main drafters of this section. Jane Guiliang, Sun Wenji, PIDM, Lei Yan, Zou Yafei, Guo Rong.
Cotton pest resistance evaluation of technical specifications
Part 4. blight
1 Scope
This section applies to cotton resistance to Fusarium wilt resistance Identification and assessment.
2 Terms and definitions
The following terms and definitions apply to this part of the GB/T 22101 of.
2.1
Cotton seedlings incidence percentage of total number of cotton seedlings.
2.2
A comprehensive assessment of composite indicators of morbidity and severity.
Training 3 pathogens
3.1 Training pathogens basic equipment
Incubator, clean table, autoclaves, refrigerators, temperature-controlled greenhouse (keeping the temperature between 20 ℃ ~ 28 ℃), plastic
Pots, aluminum pot, electric stove, petri dishes, test tubes, scissors, tweezers, jars, lamps and other alcohol.
3.2 Identification of Fusarium oxysporum used Races
Due to Fusarium oxysporum race 7 most widely distributed, for small species should be chosen on the 7th, but can also be based around the advantages of local races, election
Optional used strains.
3.3 blight pathogen culture
Fusarium wilt (see Appendix A) bacteria cultures using sand grains or grain cultivation (wheat sand = 3/1), the first grains with flooding
Bubble more than 12h, then boiled up so far, drain water mix sand, loaded jars, heat sterilization 2h; the clean table would have been
Develop good Fusarium oxysporum tablet or access ramp which then placed in an incubator at 25 ℃ cultured 7d ~ 10d.
3.4 Identification of soil inoculated with the disease
With disease-free soil sifted through 160 ℃ dry heat sterilization. Then, the bacteria culture grain or grain of sand soil by weight of 2% to 3% compared
Cases were added to sterilized soil, mix well. With clean newspaper rolled into a diameter of 6cm, 8cm high bottomed pot, which has been mixed uniformly contaminated
Soil load bowl, to 2/3 height, then loaded into 30cm × 20cm × 9cm plastic pot and set aside.
3.5 Identification of material cultivation methods
Identification of the material grown in the greenhouse using pots potting soil inoculation method. 3 replicates of 12 pot pot, each identified material 3 pots,
A total of 36 bowl; the seeds before planting pot before pouring 300mL of tap water to make mortar soil suck enough water, then have to be primed first identified species
5% of the fungicide carbendazim mix, then placed in the bowl, each bowl 6 ~ 8 cottonseed; then cover with sterile soil (height and bowl flush), then poured
Into 200mL water.
3.6 standard control
Identification and selection of a susceptible control a disease control, disease control use of "No. 86-1," susceptible control can be used, "No. 11 Ji cotton"
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