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GB/T 21809-2008: Chemicals -- Test method of earthworm, acute toxicity test
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21809-2008 | English | 174 |
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Chemicals -- Test method of earthworm, acute toxicity test
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GB/T 21809-2008
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Basic data | Standard ID | GB/T 21809-2008 (GB/T21809-2008) | | Description (Translated English) | Chemicals -- Test method of earthworm, acute toxicity test | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 13.020.40 | | Word Count Estimation | 9,978 | | Date of Issue | 2008-05-12 | | Date of Implementation | 2008-09-01 | | Adopted Standard | OECD No.207-1984, IDT | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 7, 2008 (No. 120 overall) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemical earthworm acute toxicity test substance information, methodologies outlined, equipment, test methods, data and reports. This standard applies to the evaluation of the earthworm acute toxicity of chemicals. | GB/T 21809-2008: Chemicals -- Test method of earthworm, acute toxicity test---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Test method of earthworm, acute toxicity test
ICS 13.300; 13.020.40
A80
National Standards of People's Republic of China
Chemicals Earthworm Acute Toxicity Test
Posted 2008-05-12
2008-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard is identical with the Organisation for Economic Co-operation and Development (OECD) guidelines for testing of chemicals No. 207 (1984), "Acute Toxicity earthworms
test".
Appendix A of this standard is an informative annex.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard is drafted by. China Inspection and Quarantine Science Research Institute.
Participated in the drafting of this standard. Liaoning CIQ.
The main drafters of this standard. Yu Wen Lin, Falls Apart, Wang Junbing, Zhou Xin, Hao Nan, Liu Haibo, Zhou Chuan, Liu Weidong, Sun Xin, Wang Zheng.
Chemicals Earthworm Acute Toxicity Test
1 Scope
This standard specifies the test substance chemical information Earthworm acute toxicity test, the method outlined, equipment, test methods, data and
report.
This standard applies to the evaluation of chemicals earthworm acute toxicity.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Median lethal concentration LC50
During the test to 50% of the test subjects concentrations of biological death.
In contact with the test strip, the test substance in mg/cm2 to represent; in artificial soil test to mg/kg (dry weight) represents.
3 test substance information
a) solubility in water;
b) vapor pressure;
c) structural formula;
d) purity;
e) Stability in water, soil and light in;
f) n-octanol/water partition coefficient (POW);
g) biodegradation test results.
4 Summary of Method
4.1 Introduction
This method is applicable to water-soluble and water-insoluble substances, but with different testing methods.
Test chemical toxicity earthworms have many methods, including spot-on, forced feeding and immersion tests. The method includes two Test side
Method. contact filter paper test and artificial soil toxicity test.
Simple filter paper in contact toxicity test as a pre-test, the test substance can be drawn from the soil may be toxic to earthworms, determine the need for
Further artificial soil test.
Contacting the test is simple, easy to operate, using the recommended biological test results reproducible. Toxicity data derived from artificial soil test
Representative of earthworms in the natural chemical exposure cases.
Mortality in the control group at the end of the test must not exceed 10%, the test is valid.
4.2 reference material
To determine LC50 should select a reference material, to ensure that the laboratory test conditions is sufficient and there is no significant change. Chloroacetamide
(Chloracetamide) is the appropriate reference.
4.3 Test principle
Pre-test (contact filter paper Toxicity Test) and the earthworm wet paper contact with the test substance, to identify potentially toxic soil of earthworms
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