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GB/T 21799-2008: Chemicals -- Test method of mouse spot
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| GB/T 21799-2008 | English | 194 |
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Chemicals -- Test method of mouse spot
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GB/T 21799-2008
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Basic data | Standard ID | GB/T 21799-2008 (GB/T21799-2008) | | Description (Translated English) | Chemicals -- Test method of mouse spot | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 5,583 | | Date of Issue | 2008-05-12 | | Date of Implementation | 2008-09-01 | | Adopted Standard | OECD No.484-1986, IDT | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 7, 2008 (No. 120 overall) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the mouse spot test range, test the basic principles, test methods, test data and reports. This standard applies to detect chemicals induced somatic mutations. | GB/T 21799-2008: Chemicals -- Test method of mouse spot---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals.Test method of mouse spot
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemical mouse spot test method
Posted.2008-05-12
2008-09-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard is identical with the Organization for Economic Co-operation and Development (OECD) Guide to Testing Chemicals. 484 (1986) "Genotoxicity. small
Mouse Spot Test "(in English).
This standard made the following editorial changes.
--- Increased range of parts;
--- Unit of measurement into China's legal units of measurement;
--- Removed the introduction.
This standard by the National Standardization Technical Committee of hazardous chemicals management (SAC/TC251) and focal point.
This standard is drafted by. Tianjin Inspection and Quarantine Science and Technology Institute.
The main drafters of this standard. Zhang Yuan, Wang Hua, Feng Zhizhi, Li Xue Yang, Zhao Zhuo, Yu Zhi Rui.
Chemical mouse spot test method
1 Scope
This standard specifies the scope of the mouse spot test, test the basic principles, test methods, test data and reports.
This standard applies to the detection of chemical-induced mouse somatic mutations.
2 test the basic principles
This is an in vivo test that exposes developing embryos to compounds. Target cells are melanocytes that develop embryos, and target genes are controlled
Fur coat pigmentation genes. Many of the fur pigment genes in developing mouse embryos are heterozygous. Into melanoma cells dominant
Allelic mutations (through a variety of genetic events) result in the expression of recessive phenotypes in the cells of their offspring so that in the test mouse's coat
In the formation of a change of color spots. The frequency of occurrence of such spots in the test group was then compared with that in the control group.
3 test methods
3.1 Test sample
Test substance should be dissolved or suspended in isotonic saline solution. Water-insoluble compounds can be dissolved or suspended in suitable excipients
in. The excipients used should neither interfere with the test substance, nor should it produce toxic effects. The test substance should be used before the fresh configuration.
3.2 Test animals
T-type mice (non-tachycardia, a/a; squirrel, pink eye, cchp/cchp; brown, b/b; light, short, dse/dse;
In combination with the HT line (light, non-agouti, short foot, paabp/paabp; lead gray fluff, 1nfz/1nfz; blue gray pe/pe) or C57/B1
Mouse, a/a) mating. Other suitable hybridizations such as NMRI (non-tachycardia, a/a; albino, c/c) and DBA (non-tachycardia, a/a;
Color d/d) Non-agouti mice can also be used.
3.3 Quantity and gender
Adequate exposed pregnant mice should be used in each dose group to breed a sufficient number of surviving mice. By observing the number of spots in the treatment group and
The proportion of control group to determine the size of the sample.
3.4 exposure routes
Commonly used route of exposure for oral administration of intragastric and intraperitoneal injection of pregnant rats, if necessary, can also be inhaled or other appropriate routes of exposure. Abdominal cavity
Injections may be more suitable for identifying congenital mutations. Exposure to human exposure to the same way, the resulting test results on the risk assessment
Set the most practical.
3.5 dose
Use at least two appropriately spaced dosages, including a dose that produces signs of toxic effects or reduces the number of pups. Test substance
Relatively non-toxic when the test dose should reach 1g/(kg · d), if such a high dose can not be reached, the highest dose should be tested.
3.6 control
An excipient-only negative control should be given in conjunction with the exposed mice. Historical data from the same mouse from the same laboratory is also available
as comparison. Satisfactory positive control results from the same laboratory in the near future (usually not more than 12 months) can also be used instead of synchronization
Positive result.
3.7 exposure procedures
Pregnant rats are usually exposed once on the 8th, 9th and 10th day of conception, and the day when the vaginal suppository is found is counted as the first day. These days and
7.25 days after conception, 8.25 days and 9.25 days. It is also possible to continue the exposure for these days.
3.8 results observed
Mice were numbered and counted 3 to 4 weeks after birth in mice spots, divided into three categories.
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