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GB/T 21768-2008: Chemical -- Test method of DNA damage and repair/unscheduled DNA synthesis with mammalian cells in vitro
Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21768-2008 | English | 279 |
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Chemical -- Test method of DNA damage and repair/unscheduled DNA synthesis with mammalian cells in vitro
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GB/T 21768-2008
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Basic data | Standard ID | GB/T 21768-2008 (GB/T21768-2008) | | Description (Translated English) | Chemical -- Test method of DNA damage and repair/unscheduled DNA synthesis with mammalian cells in vitro | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 7,717 | | Date of Issue | 2008-05-12 | | Date of Implementation | 2008-09-01 | | Adopted Standard | OECD No.482-1986, IDT | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 7, 2008 (No. 120 overall) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemicals in vitro mammalian cell DNA damage and repair unscheduled DNA synthesis test the scope, terms and definitions, test basic principles, test methods, test data and reports. This standard applies to detect mammalian cells can induce DNA damage and repair chemicals. | GB/T 21768-2008: Chemical -- Test method of DNA damage and repair/unscheduled DNA synthesis with mammalian cells in vitro
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemical. Test method of DNA damage and repair/unscheduled DNA synthesis with mammalian cells in vitro
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemicals in vitro mammalian cell DNA damage
Repair/unscheduled DNA synthesis test methods
Posted 2008-05-12
2008-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard is identical with the Organisation for Economic Co-operation and Development (OECD) Chemicals testing guidelines No. 482 (1986) "in vitro mammalian
Cellular DNA damage and repair/unscheduled DNA synthesis test "(in English).
The editorial changes made the following standard.
--- Increasing the scope section;
--- Change the unit of measure of legal units of measurement;
--- Delete the reference section of the OECD.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard is drafted by. China Center for Disease Control and Prevention of Occupational Health and Poison Control.
Participated in the drafting of this standard. Guangdong CIQ, Tianjin Inspection and Quarantine Institute of Science and Technology.
The main drafters of this standard. Liuqing Jun, Cheng Shujun, Xuchong Hui, Ren Jun, Zhang Yuan.
Chemicals in vitro mammalian cell DNA damage
Repair/unscheduled DNA synthesis test methods
1 Scope
This standard specifies the chemical in vitro mammalian cell DNA damage and repair unscheduled DNA synthesis test the scope, terms and
Definitions, test the basic principles, test methods, test data and reports.
This standard is applicable to the detection of mammalian cells can induce DNA damage and repair chemicals.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Under normal circumstances, DNA synthesis only in the mitotic cycle S phases, known as DNA synthesis.
2.2
Chemical or physical agents cause DNA damage, cut, and remove damaged DNA fragments after repair synthesis.
3 test basic principles
Mammalian cells in vitro unscheduled DNA synthesis test (UDS) describes the use of primary cells or continuous mammalian cell line inspection
Measuring DNA repair synthesis method of operation. End unscheduled DNA synthesis can be measured by autoradiography of radiolabeled
Nucleosides, such as tritiated thymidine (3H-TdR) uptake to, scintillation counting, or may also be (LSC) by liquid
Measured. UDS may also be used in vivo experimental system were measured.
After the chemical or physical factors induce DNA damage, cells start unscheduled DNA synthesis procedures to excise or remove the damaged DNA region
Domain, UDS test is to detect damaged DNA repair synthesis. Principle of the test is to detect 3H-TdR incorporation of non-mammalian S
The amount of cellular DNA. Autoradiography techniques or by liquid scintillation counting radioactive DNA (LSC) method for the determination of the exposed cells of 3H-TdR
Absorption. In addition to using primary rat liver cells as target cells, cultured mammalian cells should be exogenous metabolic live in Canada and without
Under System and the role of the test substance in both cases.
Test Method 4
4.1 test substance required information
a) states of matter. solid, liquid or gas;
b) The name and identification code;
c) purity (impurities);
d) dissolution characteristics;
e) Melting point/boiling point;
f) pH value (measured at);
g) vapor pressure data (if available).
4.2 Test preparation
4.2.1 test substance
Test substance and controls should be used to prepare cell culture media, or first dissolved in a suitable vehicle, and further diluted with cell culture medium using
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