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GB/T 21167-2007 English PDF

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GB/T 21167-2007: Determination of nitrofuran metabolites residues in royal jelly -- LC-MS-MS method
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Basic data

Standard ID GB/T 21167-2007 (GB/T21167-2007)
Description (Translated English) Determination of nitrofuran metabolites residues in royal jelly -- LC-MS-MS method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B47
Classification of International Standard 67.180.10
Word Count Estimation 9,997
Date of Issue 2007-10-31
Date of Implementation 2008-04-01
Quoted Standard GB/T 6682
Regulation (derived from) China National Standard Approval Announcement2007 No.12 (Total No.112)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the royal jelly furaltadone metabolite 5-methyl morpholin-3-amino-2-oxazolidinylethyl one, nitrofurazone metabolite semicarbazide (, nitrofurantoin metabolites object 1-amino-2-within hydantoin and furazolidone metabolite 3-amino-2-yl alkyl ketones residue liquid chromatography-tandem mass spectrometry determination of the criteria applicable to the determination of nitrofuran metabolite residues in royal jelly.

GB/T 21167-2007: Determination of nitrofuran metabolites residues in royal jelly -- LC-MS-MS method


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Determination of nitrofuran metabolites residues in royal jelly. LC-MS-MS method ICS 67.180.10 B47 National Standards of People's Republic of China Determination of royal jelly nitrofuran metabolites residues Liquid chromatography - tandem mass spectrometry Posted 2007-10-31 2008-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Ding Tao, Xu Jinzhong, Shen Chong-yu, Wu Bin, Chen Huilan, by Zhao Yun, Zhang Yang, ZHU Chun, Jiang Yuan, Wang Jin Tao. Determination of royal jelly nitrofuran metabolites residues Liquid chromatography - tandem mass spectrometry

1 Scope

This standard specifies the royal jelly furaltadone metabolite 5-methyl-morpholino-3-amino -2- oxazolidinyl ketone (referred AMOZ), furan Metabolism Xilin metabolite semicarbazide (abbreviated SEM), nitrofurantoin metabolite amino-2-hydantoin (abbreviated AHD) and furazolidone Was 3-amino -2- oxazolidinyl ketone (referred AOZ) residual amount of liquid chromatography - tandem mass spectrometry measurement. This standard applies to the determination of nitrofuran metabolites residues in royal jelly.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

Nitrofuran drugs rapid decomposition in animals, which is only a few hours in situ stability, and its metabolites in the body of an animal egg and easy White tissue binding, very stable. Trichloroacetic acid solution was precipitated protein in royal jelly, the o-nitrobenzaldehyde derivative as a derivative reagent dark Biochemical 16h, ethyl acetate extraction and concentration by liquid chromatography - tandem mass spectrometer, internal standard.

4 Reagents and materials

4.1 Water. Water should be consistent with a GB/T 6682 regulations. 4.2 Methanol. HPLC grade. Ethyl acetate 4.3. HPLC grade. Ammonium acetate 4.4. HPLC grade. 4.5 TCA. AR. Anhydrous dipotassium hydrogen 4.6. AR. 4.7 DMSO. AR. 4.8 o-nitrobenzaldehyde. AR. 4.9 isotope internal standard. AHD-13C3, AMOZ-D5, AOZ-D4, SEM · HCl- (13C, 15N2), 100μg/mL, Purity ≥98%. 4.10 AOZ, SEM · HCl, AMOZ and AHD · HCl Standard. Purity ≥98%. 4.11 AOZ, SEM, AMOZ and AHD standard stock solution. 1mg/mL. Accurately weighed amount of AOZ, SEM · HCl, AMOZ and AHD · HCl standard (4.10), with methanol (4.2) standard stock solution dubbed 1.0mg/mL of. Stock solution stored in 4 ℃ refrigerator, valid for one year. 4.12 AOZ, SEM, AMOZ and AHD standard solution. mobile phase were formulated at a concentration of 100ng/mL, 10ng/mL standard Quasi working solution, standard solution at 4 ℃ preservation, valid for 3 months. 4.13 Internal standard solution. mobile phase formulated at a concentration of 50ng/mL mixed solution of four internal standard, internal standard mixed solution was stored at 4 ℃, 1 Year effective.

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