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GB/T 21164-2007 English PDF

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GB/T 21164-2007: Determination of streptomycin, dihydrostreptomycin residues in royal jelly -- Liquid chromatography method
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GB/T 21164-2007English189 Add to Cart 3 days [Need to translate] Determination of streptomycin, dihydrostreptomycin residues in royal jelly -- Liquid chromatography method Valid GB/T 21164-2007

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Basic data

Standard ID GB/T 21164-2007 (GB/T21164-2007)
Description (Translated English) Determination of streptomycin, dihydrostreptomycin residues in royal jelly -- Liquid chromatography method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B47
Classification of International Standard 67.180.10
Word Count Estimation 7,759
Date of Issue 2007-10-31
Date of Implementation 2008-04-01
Quoted Standard GB/T 6682
Regulation (derived from) China National Standard Approval Announcement2007 No.12 (Total No.112)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the royal jelly streptomycin and dihydrostreptomycin residual liquid chromatography �� column derivatization fluorescence spectrometry. This standard applies to the determination of streptomycin and dihydrostreptomycin residues in royal jelly.

GB/T 21164-2007: Determination of streptomycin, dihydrostreptomycin residues in royal jelly -- Liquid chromatography method


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Determination of streptomycin, dihydrostreptomycin residues in royal jelly. Liquid chromatography method ICS 67.180.10 B47 National Standards of People's Republic of China Royal jelly streptomycin, dihydrostreptomycin residues in the determination Liquid chromatography Posted 2007-10-31 2008-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, China Veterinary Drug Control. The main drafters of this standard. Chen Huilan, Wu Bin, Zhao Yun increased, Chong Yu Shen, Ding Tao, Huang Juan, Zhucheng Jing, Xu Jinzhong, Jiang Yuan, Wang Jin Tao, Zhu Citadines. Royal jelly streptomycin, dihydrostreptomycin residues in the determination Liquid chromatography

1 Scope

This standard specifies the royal jelly streptomycin and dihydrostreptomycin Liquid Chromatography - post-column derivatization fluorescence spectrometry. This standard applies to the determination of streptomycin and dihydrostreptomycin residues in royal jelly.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

Royal jelly samples streptomycin and dihydrostreptomycin with heptane sulfonate - mixed extract sodium phosphate extraction, extract with C18 solid phase extraction Column purification, methanol, rotary evaporated to dryness and 0.01mol/L sodium heptane solution dissolved with the sample solution by high performance liquid chromatography - Post-column derivatization, fluorescence detection, external standard.

4 Reagents and materials

4.1 Methanol. chromatographically pure. 4.2 Water. Water should be consistent with a GB/T 6682 regulations. 4.3 acetonitrile. chromatography. 4.4 Sodium hydroxide. AR. 4.5 chloroform. AR. 4.6 1,2-naphthoquinone-4-sulfonate. chromatography. 4.7 tert-butyl methyl ether. chromatography. 4.8 hexane. AR. 4.9 sodium heptane. chromatography. 4.10 Sodium. AR. 4.11 Phosphate. AR. 4.12 streptomycin and dihydrostreptomycin standard substance. purity ≥99%. 4.13 C18 solid phase extraction column. 500mg, 3mL. Before use 5mL methanol 10mL water pre-wash and keep moist column. 4.14 heptane sodium - sodium phosphate mixed extract. Weigh 10.10g sodium heptane, 4.10g of sodium dissolved in 1000mL of water, PH3.3 solution was adjusted with phosphoric acid (4.11). 4.15 tert-butyl methyl ether - n-hexane solution. n-hexane of 10mL, 40mL tert-butyl methyl ether, and mix. 4.16 sodium heptane solution. 0.01mol/L. Weigh 2.02g of heptane sulfonate, was dissolved in 1000mL of water (4.11) adjusted with phosphoric acid pH3.3. 4.17 Sodium hydroxide solution. 0.2mol/L. Weigh 8g sodium hydroxide (4.4), was dissolved in 1000mL of water, through 0.45μm membrane, Suction degassing.

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