|
US$249.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 19540-2025: Determination of zearalenone in feeds
Status: Valid GB/T 19540: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 19540-2025 | English | 249 |
Add to Cart
|
3 days [Need to translate]
|
Determination of zearalenone in feeds
| Valid |
GB/T 19540-2025
|
| GB/T 19540-2004 | English | 189 |
Add to Cart
|
3 days [Need to translate]
|
Determination of zearalenone in feeds
| Valid |
GB/T 19540-2004
|
PDF similar to GB/T 19540-2025
Basic data | Standard ID | GB/T 19540-2025 (GB/T19540-2025) | | Description (Translated English) | Determination of zearalenone in feeds | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 12,169 | | Date of Issue | 2025-06-30 | | Date of Implementation | 2026-01-01 | | Older Standard (superseded by this standard) | GB/T 19540-2004, GB/T 28716-2012 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 19540-2025: Determination of zearalenone in feeds---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT19540-2025
National Standard of the People's Republic of China
ICS 65.120CCS B 46
Determination of Zearalenone in Feed
Released on June 30, 2025
2026-01-01 implementation
State Administration for Market Regulation
The National Standardization Administration issued
Replaces GB/T 19540-2004 and GB/T 28716-2012
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 19540-2004 “Determination of zearalenone in feeds” and GB/T 28716-2012 “Determination of zearalenone in feeds”.
Determination of mithralenone by immunoaffinity column cleanup - high performance liquid chromatography.
Compared with.2012, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application has been changed (see Chapter 1 of GB/T 19540-2004 and Chapter 1 of GB/T 28716-2012);
b) Thin layer chromatography and enzyme-linked immunosorbent assay (ELISA) have been deleted (see Chapter 3 and Chapter 4 of GB/T 19540-2004);
c) Liquid chromatography-tandem mass spectrometry has been added (see Chapter 4).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. Shanghai Academy of Agricultural Sciences and Shanghai Animal Disease Prevention and Control Center.
The main drafters of this document are. Han Zheng, Cao Ying, Zhao Zhihui, Nie Dongxia, Guo Dakai, Wang Xinyi, Wu Jianping, Yang Haifeng, Guo Wenbo, Zhang Jing,
Yan Feng, Huang Qingwen, Fan Kai, Shang Jun, Wu Yushan.
The previous versions of this document and the documents it replaces are as follows.
--Published as GB/T 19540-2004 in.2004 and GB/T 28716-2012 in.2012;
--This is the first revision.
Determination of Zearalenone in Feed
1 Scope
This document describes a liquid chromatography-tandem mass spectrometry and high performance liquid chromatography method for the determination of zearalenone in feed.
This document applies to the determination of zearalenone in compound feed, concentrate supplements, concentrate feed and plant feed ingredients.
The detection limit of this document is 2 μg/kg and the quantification limit is 10 μg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Liquid chromatography-tandem mass spectrometry
4.1 Principle
The analytes in the sample were extracted with acetonitrile solution, the extract was diluted with phosphate buffer solution, purified by immunoaffinity column, and analyzed by liquid chromatography-mass spectrometry.
The samples were determined by tandem mass spectrometry, calibrated by matrix-matched standard curve, and quantified by external standard method.
4.2 Reagents or materials
Warning - Zearalenone is highly toxic. Avoid contact with skin and clothing during operation and wear medical latex gloves.
Handle the reagents carefully and use them in a fume hood.
Unless otherwise specified, only analytical grade reagents were used.
4.2.1 Water. GB/T 6682, Grade 1.
4.2.2 Methanol. chromatographic grade.
4.2.3 Acetonitrile. chromatographic grade.
4.2.4 Formic acid. chromatographically pure.
4.2.5 Sodium chloride.
4.2.6 Sodium hydroxide solution (0.2 mol/L). Weigh 8.0 g of sodium hydroxide, dissolve it in water, transfer it to a 1000 mL volumetric flask, make up to volume, and mix thoroughly.
4.2.7 80% acetonitrile solution. Measure 800 mL of acetonitrile (4.2.3) into a 1000 mL volumetric flask, dilute with water, make up to volume, and mix thoroughly.
4.2.8 0.1% formic acid solution. Pipette 1 mL of formic acid (4.2.4) into a 1000 mL volumetric flask, dilute with water, make up to volume, and mix thoroughly.
4.2.9 Phosphate buffer solution (PBS). weigh 8.0 g sodium chloride, 1.16 g disodium hydrogen phosphate, 0.2 g potassium dihydrogen phosphate, and 0.2 g potassium chloride.
Dissolve and dilute with water to 1000 mL, and adjust the pH to 7.4 with sodium hydroxide solution (4.2.6).
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 19540-2025_English be delivered?Answer: Upon your order, we will start to translate GB/T 19540-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 19540-2025_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 19540-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. Question 5: Should I purchase the latest version GB/T 19540-2025?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 19540-2025 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.
|