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GB/T 19423-2020: Determination of nicarbazin in feeds
Status: Valid GB/T 19423: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 19423-2020 | English | 209 |
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Determination of nicarbazin in feeds
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GB/T 19423-2020
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| GB/T 19423-2003 | English | 199 |
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Determination of nicarbazin in feed -- High performance liquid chromatography
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GB/T 19423-2003
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PDF similar to GB/T 19423-2020
Basic data | Standard ID | GB/T 19423-2020 (GB/T19423-2020) | | Description (Translated English) | Determination of nicarbazin in feeds | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 11,146 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2021-07-01 | | Older Standard (superseded by this standard) | GB/T 19423-2003 | | Quoted Standard | GB/T 6682; GB/T 20195 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry methods for the determination of Nicarbazine in feed. This standard is applicable to the determination of Nicarbazine in compound feed, concentrated feed and additive premix feed. In this standard HPLC method, the detection limit of compound feed and concentrated feed is 0.5mg/kg, the limit of quantification is 1mg/kg, the detection limit of additive premixed feed is 1mg/kg, and the limit of quantification is 2mg/kg; The detection limit of liquid chromatography-tandem mass spectrometry is 0.02mg/kg, and the limit of quantification is 0.05mg/kg. | GB/T 19423-2020: Determination of nicarbazin in feeds---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of Nicarbazine in Feed)
ICS 65:120
CCSB46
National Standards of People's Republic of China
Replace GB/T 19423-2003
Determination of Nicarbazine in Feed
2020-12-14 release
2021-07-01 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1:1-2020 Standardization Guidelines Part 1: Structure and Drafting Rules of Standardization Documents
Drafting:
This document replaces GB/T 19423-2003 "Determination of Nicarbazine in Feed High-Performance Liquid Chromatography", and GB/T 19423-
Compared with:2003, in addition to editorial changes, the main technical changes are as follows:
---Added liquid chromatography-tandem mass spectrometry method (see Chapter 5);
---Changed the detection limit of high performance liquid chromatography and increased the limit of quantification (see Chapter 1, Chapter 1 of the:2003 edition);
---Changed the extraction and purification methods of high performance liquid chromatography (see 4:5:1, 4:5:2, 7:1 in the:2003 edition);
---Changed the test data processing of high performance liquid chromatography (see 4:6, 7:4 in the:2003 edition):
Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying patents:
This document was proposed and managed by the National Feed Industry Standardization Technical Committee (SAC/TC76):
Drafting organizations of this document: Agricultural Products Public Monitoring Center of Guangdong Academy of Agricultural Sciences, Zhejiang Veterinary Drug Feed Supervision Institute, Guizhou Veterinary Drug Feed
Material testing institute:
The main drafters of this document: Wang Weili, Ding Chenhong, Zhang Zhijian, Zhao Gui, Wan Kai, Wang Xu, Li Yafei, Su Qiuquan, Lin Xuexian, Zhang Lijie,
Hou Xuan, Huang Xiaomei, Yin Qiumiao, Liang Rui, Cui Zefeng, Xie Lili, Wang Ying:
The previous versions of this document and the documents replaced are as follows:
---GB/T 19423-2003:
Determination of Nicarbazine in Feed
1 Scope
This document describes the high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry methods for the determination of Nicarbazine in feed:
This document is applicable to the determination of Nicarbazine in compound feed, concentrated feed and additive premix feed:
The detection limit of compound feed and concentrated feed in this document is 0:5mg/kg, and the limit of quantification is 1mg/kg: Additives
The detection limit of premixed feed is 1 mg/kg, and the limit of quantification is 2 mg/kg; the detection limit of liquid chromatography-tandem mass spectrometry is 0:02 mg/kg,
The limit is 0:05mg/kg:
2 Normative references
The contents of the following documents constitute indispensable clauses of this document through normative references in the text: Among them, dated quotations
Only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document:
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms and definitions that need to be defined in this document:
4 High performance liquid chromatography
4:1 Principle
Nicarbazine in the sample is extracted with a methanol-acetonitrile mixed solution, purified by MAX solid phase extraction cartridge, and then used by high performance liquid chromatography
Detect and quantify by external standard method:
4:2 Reagents or materials
Unless otherwise specified, only use analytical reagents:
4:2:1 Water: GB/T 6682, level one:
4:2:2 Acetonitrile: chromatographically pure:
4:2:3 Extraction solution: methanol acetonitrile = 50 50:
4:2:4 80% acetonitrile solution: accurately measure 80mL acetonitrile (4:2:2), add water to the volume to 100mL, and mix:
4:2:5 Sodium hydroxide solution (0:2mol/L): accurately measure 0:8g of sodium hydroxide, dilute with water and dilute to 100mL, and mix:
4:2:6 4% formic acid acetonitrile solution: accurately measure 4mL formic acid, dilute with acetonitrile (4:2:2) and dilute to 100mL, and mix:
4:2:7 Standard stock solution (1000μg/mL): Weigh Nicarbazin (Nicarbazin, C19H18N6O6, CAS number: 330-95-0, content
Not less than 99%) standard 10mg (accurate to 0:01mg) in a 10mL volumetric flask, add 2mL dimethyl sulfoxide to dissolve, use acetonitrile
(4:2:2) Constant volume, shake well, store below -18℃, validity period is 3 months:
4:2:8 Standard intermediate solution (100μg/mL): accurately measure 1mL of the standard stock solution (4:2:7) and place it in a 10mL volumetric flask:
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