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GB/T 19200-2025: Diagnostic techniques for swine vesicular disease
Status: Valid GB/T 19200: Evolution and historical versions
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| GB/T 19200-2025 | English | 439 |
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Diagnostic techniques for swine vesicular disease
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GB/T 19200-2025
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| GB/T 19200-2003 | English | 319 |
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Diagnostic techniques for swine vesicular disease
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GB/T 19200-2003
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Basic data | Standard ID | GB/T 19200-2025 (GB/T19200-2025) | | Description (Translated English) | Diagnostic techniques for swine vesicular disease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 22,280 | | Date of Issue | 2025-08-29 | | Date of Implementation | 2026-03-01 | | Older Standard (superseded by this standard) | GB/T 19200-2003, GB/T 22917-2008 | | Issuing agency(ies) | State Administration for Market Regulation, National Standardization Administration | GB/T 19200-2025: Diagnostic techniques for swine vesicular disease---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT19200-2025
ICS 11.220
CCSB41
National Standard of the People's Republic of China
Replaces GB/T 19200-2003, GB/T 22917-2008
Diagnostic technology of swine vesicular disease
Released on August 29, 2025
Implementation on March 1, 2026
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Clinical Diagnosis 2
6 Biosafety Measures 2
7 Sample Collection and Processing 2
8 Virus Isolation 3
9 Reverse transcription-polymerase chain reaction (RT-PCR) 5
10 Fluorescent reverse transcription polymerase chain reaction (fluorescent RT-PCR) 6
11 Virus Neutralization Test (VNT) 8
12 Monoclonal antibody competition enzyme-linked immunosorbent assay (monoclonal antibody competition ELISA) 10
13 Comprehensive judgment 11
Appendix A (Normative) Reagents and Solution Preparation Methods 12
Appendix B (Informative) Preparation of positive control plasmid for RT-PCR detection and test results (see Figure 13)
Appendix C (Informative) Preparation of positive control plasmid for fluorescent RT-PCR detection and detection results refer to Figure 14
Appendix D (Informative) Preparation of Standard Positive Serum and Standard Negative Serum 15
Appendix E (Normative) Karber method calculation method 16
Appendix F (Normative) Enzyme-linked immunosorbent assay solution preparation method 17
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 19200-2003 “Diagnostic Techniques for Swine Vesicular Disease” and GB/T 22917-2008 “Fluorescence Spectroscopy of Swine Vesicular Disease Virus”.
RT-PCR detection method". This document is based on GB/T 19200-2003 and integrates the contents of GB/T 22917-2008.
Compared with GB/T 19200-2003, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
--- Changed the scope (see Chapter 1, Chapter 1 of the.2003 edition);
--- Added abbreviations (see Chapter 4);
--- Increased clinical diagnosis (see Chapter 5);
--- Added biosafety measures (see Chapter 6);
--- Added reverse transcription-polymerase chain reaction (RT-PCR) (see Chapter 9);
--- Added fluorescent reverse transcription polymerase chain reaction (fluorescent RT-PCR) (see Chapter 10);
--- Added monoclonal antibody competition enzyme-linked immunosorbent assay (monoclonal antibody competition ELISA) (see Chapter 12);
--- Added comprehensive judgment (see Chapter 13);
--- Deleted the reverse indirect hemagglutination test (RIHA) (see 2.5 of the.2003 edition);
--- Deleted agar gel immunodiffusion test (AGID) (see Chapter 3 of the.2003 edition).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181).
This document was drafted by. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, and Shenzhen Customs Animal and Plant Inspection and Quarantine Technical Center.
The main drafters of this document are. Zheng Haixue, Yang Fan, He Jijun, Ma Weimin, Shen Chaochao, Cao Weijun, Ruan Zhouxi, Zhu Zixiang, Yang Yamin, Lü Lu,
Liu Yongjie, Lin Yanxing, Hua Qunyi, Guo Jianhong, and Liu Xiangtao.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2003 as GB/T 19200-2003;
--- This is the first revision, incorporating the technical content of GB/T 22917-2008.
introduction
An acute, contagious disease of pigs characterized by blisters around the hooves, mouth, snout, and teats, with no obvious signs of infection in the internal organs.
SVDV belongs to the Picornaviridae family, Enterovirus genus, and has only one
The World Organization for Animal Health (WOAH) lists it as a notifiable disease, and my country defines it as a Class I animal disease.
Diagnostic technology of swine vesicular disease
1 Scope
This document describes the clinical diagnosis of swine vesicular disease, laboratory diagnostic sample collection and processing, as well as virus isolation, reverse transcription-polymerase chain reaction
Laboratory diagnostic methods include PCR, fluorescent reverse transcription-polymerase chain reaction, virus neutralization test, monoclonal antibody competitive enzyme-linked immunosorbent assay, etc.
This document applies to the diagnosis, quarantine, detection, monitoring and epidemiological investigation of swine vesicular disease.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB 19489 General requirements for laboratory biosafety
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
BEI. Binaryethylenimine
OD. Optical density
RNA. Ribonucleic acid
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