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GB/T 18638-2021: Diagnostic techniques for Japanese encephalitis
Status: Valid GB/T 18638: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18638-2021 | English | 559 |
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Diagnostic techniques for Japanese encephalitis
| Valid |
GB/T 18638-2021
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| GB/T 18638-2002 | English | 519 |
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Diagnostic technique of epidemic encephalitis B
| Obsolete |
GB/T 18638-2002
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PDF similar to GB/T 18638-2021
Basic data | Standard ID | GB/T 18638-2021 (GB/T18638-2021) | | Description (Translated English) | Diagnostic techniques for Japanese encephalitis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 30,334 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 18638-2021: Diagnostic techniques for Japanese encephalitis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for Japanese encephalitis
ICS 11.220
B41
National Standards of People's Republic of China
Replace GB/T 18638-2002
Diagnosis technology of Japanese encephalitis
Released on 2021-03-09
2021-10-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Table of contents
Foreword Ⅰ
Introduction Ⅱ
1 Scope 1
2 Normative references 1
3 Abbreviations 1
4 Clinical diagnosis 1
5 Laboratory diagnostic sample collection and processing 2
6 Virus isolation and identification 3
7 Virus RT-PCR detection method 5
8 Complement fixation test detection method 7
9 Detection method of hemagglutination inhibition test 9
10 Indirect ELISA antibody detection method 11
11 Indirect immunofluorescence test (IFA) detection method 13
12 Virus neutralization test detection method 14
13 Comprehensive decision 16
Appendix A (Normative Appendix) Virus RT-PCR Detection Method Test Reagent Preparation 17
Appendix B (Normative Appendix) Reagent preparation for complement fixation test detection method and hemagglutination inhibition test detection method 18
Appendix C (Normative Appendix) Preliminary Tests for Complement Fixation Test Methods 20
Appendix D (informative appendix) Complement fixation test detection method and hemagglutination inhibition test detection method result judgment diagram 23
Appendix E (Normative Appendix) Indirect ELISA antibody detection method Test reagent preparation 24
Appendix F (Normative Appendix) Indirect immunofluorescence test (IFA) detection method Test reagent preparation 26
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces GB/T 18638-2002 "Diagnostic Techniques for Japanese Encephalitis", and compared with GB/T 18638-2002, except for editing
In addition to the modification, the main technical changes are as follows.
--- Added "Normative Reference Documents" (see Chapter 2);
---Added "abbreviations" (see Chapter 3);
---Added "viral RT-PCR detection method" (see Chapter 7);
---Added "indirect ELISA antibody detection method" (see Chapter 10);
---Added "indirect immunofluorescence test (IFA) detection method" (see Chapter 11);
--- Added "Virus Neutralization Test Detection Method" (see Chapter 12);
---Added reagent preparation for virus RT-PCR detection method test (see Appendix A);
--- Added a schematic diagram of the results of the complement fixation test detection method and hemagglutination inhibition test detection method (see Appendix D);
---Added the reagent preparation for the indirect ELISA antibody detection method test (see Appendix E);
---Added the reagent preparation for the indirect immunofluorescence test (IFA) test method (see Appendix F).
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
Drafting organizations of this standard. Institute of Military Veterinary Medicine, Academy of Military Sciences, Wenzhou University, Huazhong Agricultural University, Jilin
the University.
The main drafters of this standard. Jin Ningyi, Xiao Pengpeng, Lu Huijun, Cao Shengbo, Huang Shaomei, Zhang Ying, Tian Mingyao, Li Xiao, Li Chang.
The previous versions of the standard replaced by this standard are as follows.
---GB/T 18638-2002.
Introduction
Japanese encephalitis (Japaneseencephalitis), also known as Japanese encephalitis, is a kind of Japanese encephalitis caused by the Japanese encephalitis virus
Mosquito-borne zoonoses. Horses show signs of encephalitis, pigs show miscarriage, stillbirth and orchitis, and most other livestock and poultry are invisible
dye. There is obvious seasonality, and it occurs frequently in summer and autumn. The disease is widely distributed, mainly in Asia, causing human and animal health and national economy
Great harm and loss.
The pathogen that causes this disease is Japanese encephalitis virus (Japaneseencephalitisvirus), which belongs to the Flaviviridae family
(Flaviviridae) Flavivirus is a single-stranded RNA virus. The virus particles are spherical and have a lipoprotein envelope.
It has hemagglutinating activity and can agglutinate red blood cells of chickens, ducks, geese and sheep. The virus is not strong against the outside world, is more sensitive to chemicals, and is often disinfected
The medicine has a good inactivation effect, and it is also sensitive to pancreatin, ether, chloroform and so on.
The World Organization for Animal Health (OIE) recommends the use of virus isolation and identification, immunofluorescence test, hemagglutination inhibition test, complement fixation test, blood
Diagnosis by methods such as Qingzhong and test. There are many research reports on the disease in our country, and the more commonly used diagnostic methods are still specified in this standard.
Clinical diagnosis, virus isolation and identification, hemagglutination inhibition test, complement fixation test, RT-PCR test, indirect ELISA test, indirect immunization
Fluorescence test and virus neutralization test. When performing virus isolation and identification, it is required that all sample processing should be at the corresponding level that can ensure biological safety
Operations are carried out in the laboratory; containers, articles, and experimental materials used to process samples should be effectively disinfected before being transported out of the laboratory.
The revision of this standard refers to the.2018 edition of the OIE "Manual of Diagnostic Tests and Vaccine Standards for Terrestrial Animals" (Manual of Diagnostic Tests and Vaccines).
Tests and Vaccines for Terrestrial Animals,.2018), combined with the new research results of related technologies in my country. The implementation of this standard,
It will play an important role in improving the diagnosis of Japanese encephalitis and predicting the epidemic situation and taking timely preventive measures to ensure the health of humans and animals.
Diagnosis technology of Japanese encephalitis
1 Scope
This standard specifies the technical requirements for clinical diagnosis and laboratory diagnosis of Japanese encephalitis (JE).
This standard applies to the diagnosis and quarantine of Japanese encephalitis in animals such as pigs, horses, mules, donkeys, cattle, sheep, dogs, ducks, geese and birds.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 18088 Entry and Exit Animal Quarantine Sampling
GB 19489 Laboratory Biosafety General Requirements
3 Abbreviations
The following abbreviations apply to this document.
BBS borate buffer (boratebufferedsaline)
CFT complement fixation test (complementfixationtest)
CPE cytopathic effect (cytopathiceffect)
DEPC diethylpyrocarbonate (diethylpyrocarbonate)
DNA deoxyribonucleic acid (deoxyribonucleic acid)
dNTP deoxy-ribonucleosidetriphosphate (deoxy-ribonucleosidetriphosphate)
ELISA enzyme-linked immunosorbent assay (enzymelinkedimmunosorbentassay)
FITC fluoresceinisothiocyanate (fluoresceinisothiocyanate)
HRP horseradish peroxidase (horseradishperoxidase)
JEV Japanese encephalitis virus (Japaneseencephalitisvirus)
PBS phosphate buffered saline
RNA ribonucleic acid (ribonucleicacid)
RT-PCR reverse transcription-polymerase chain reaction (reversetranscription-polymerasechain reaction)
TCID50 half tissue culture infection dose (mediantissuecultureinfectivedose)
4 Clinical diagnosis
4.1 Susceptible animals
Pigs, horses, mules, donkeys, cattle, sheep, dogs, ducks, geese and birds can be infected, and pigs are the most common.
4.2 Clinical symptoms
4.2.1 Pigs develop fever after infection, and a small number of pigs will have neurological symptoms. Pregnant sows can cause miscarriage, stillbirth, and testicular swelling in boars.
4.2.2 After the horse is infected, the body temperature increases (39℃~41℃), neurological symptoms (depression or manic restlessness), abnormal posture, shaking or turning when walking
Circle, limbs are swimming.
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