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GB/T 18636-2017: Diagnostic techniques for bluetongue
Status: Valid GB/T 18636: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18636-2017 | English | 579 |
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Diagnostic techniques for bluetongue
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GB/T 18636-2017
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| GB/T 18636-2002 | English | 559 |
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Diagnostic techniques for bluetongue
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GB/T 18636-2002
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PDF similar to GB/T 18636-2017
Basic data | Standard ID | GB/T 18636-2017 (GB/T18636-2017) | | Description (Translated English) | Diagnostic techniques for bluetongue | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 29,297 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Older Standard (superseded by this standard) | GB/T 18636-2002 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 18636-2017: Diagnostic techniques for bluetongue---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for bluetongue
ICS 11.220
B41
National Standards of People's Republic of China
Replacing GB/T 18636-2002
Blue tongue diagnosis technology
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Directory
Foreword Ⅲ
1 Scope 1
2 Normative references 1
3 clinical diagnosis 1
3.1 Epidemiology 1
3.2 Clinical symptoms 1
3.3 pathological changes 2
3.4 determine the results 2
4 virus separation 2
4.1 equipment 2
4.2 Reagents 2
4.3 Test Procedure 2
4.4 virus identification 4
4.5 results to determine 4
5 immune enzyme staining 4
5.1 equipment 4
5.2 Reagents 4
5.3 Test Procedure 4
5.4 test conditions established 5
5.5 determine the results 5
6 antigen capture enzyme-linked immunosorbent assay (AC-ELISA) 5
6.1 Equipment 5
6.2 Reagents 5
6.3 Sample 5
6.4 Experimental Design 5
6.5 Test Procedure 5
6.6 Test conditions established 6
6.7 Determination of results 6
7 stereotypes and micro-neutralization test 6
7.1 equipment 6
7.2 Reagents 6
7.3 Test Preparation 7
7.4 Screening Test Procedure 7
7.5 Test conditions established 8
7.6 Determination of results 8
8 plaque and plaque inhibition stereotypes test 8
8.1 equipment 8
8.2 Reagents
8.3 Plaque Test Procedure 9
8.4 Plaque inhibition test procedure 9
8.5 Determination of Results 9
9 Reverse Transcription Polymerase Chain Reaction (RT-PCR) 9
9.1 Equipment 9
9.2 Reagents 10
9.3 Test Procedure 10
9.4 Test conditions established 11
9.5 Determination of results 11
10 Fluorescent RT-PCR assay 11
10.1 Equipment 11
10.2 Reagents
10.3 Test Procedure 12
10.4 Threshold Setting 13
10.5 Test conditions established 13
10.6 Result Judgment 13
11 Agar immunodiffusion test (AGID) 13
11.1 Equipment 13
11.2 Reagents 13
11.3 Test Procedure 14
11.4 result judgment 14
12 competitive enzyme-linked immunosorbent assay (C-ELISA) 15
12.1 Equipment 15
12.2 Reagents 15
12.3 Test Procedure 15
12.4 Reading and Suppression Rate Calculation 16
12.5 conditions for the establishment of the test 16
12.6 Result Judgment 16
13 diagnostic results to determine 16
13.1 Suspected cases 16
13.2 confirmed cases 16
13.3 Recessive Infection 16
Appendix A (Normative) Cell culture fluid preparation 17
Appendix B (Normative) Preparation of immunoenzyme staining test solution 18
Appendix C (Normative) Enzyme-linked immunosorbent assay and determination of micro-neutralization test solution 19
Appendix D (informative) Karber method 21
Appendix E (Normative) Plaque and plaque inhibition stereotypes test 22
Appendix F (Normative) RT-PCR test solution and primer preparation 23
Appendix G (Normative) Synthesis and preparation of fluorescent RT-PCR primers and probes 24
Appendix H (Normative) Preparation of agar plates 25
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces GB/T 18636-2002 "blue tongue disease diagnostic technology", compared with GB/T 18636-2002, in addition to editorial changes,
The main technical changes are as follows.
--- Increased clinical diagnosis;
--- Increased reverse transcriptase polymerase chain reaction (RT-PCR) detection method;
--- Increased fluorescence RT-PCR detection method.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.
This standard was drafted unit. Yunnan Academy of Animal Husbandry and Veterinary Medicine, People's Republic of China Shenzhen Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Hua Chun, Zhu Jianbo, Hua Yun Yi, Xiao Lei, Song Jianling, Li Le, Gao Lin, Miao Haisheng.
This standard was last released version.
--- GB/T 18636-2002.
Blue tongue diagnosis technology
1 Scope
This standard specifies the Bluetongue (Bluetongue, BT) diagnostic techniques and test procedures.
This standard applies to ruminant blue tongue disease diagnosis, epidemiological investigation and quarantine. The methods include clinical diagnosis, pathogen isolation and
Identification, pathogenic nucleic acid detection and serological testing, among them.
--- Virus isolation Suitable for isolating Bluetongue virus (BTV) from animal blood, organs and semen;
--- Immunoenzyme staining and antigen capture enzyme-linked immunosorbent assay (AntigenCaptureEnzyme-LinkedImmunosorbent
Assay, AC-ELISA) is suitable for the identification of isolated isolates of serogroups;
--- stereotypes and micro-neutralization test and plaque and plaque inhibition stereotyping test for the identification of isolated BTV serotypes;
--- Reverse transcription polymerase chain reaction (Reverse-Transcription Polymerase Chain Reaction, RT-PCR) and fluorescence
RT-PCR for the detection of BTV nucleic acids in blood, organs and semen of animals is also suitable for the detection of isolated viruses
Serogroup identification
--- Agar immunodiffusion test (AGar) and competitive enzyme-linked immunosorbent assay
(CompetitionEnzyme-Linked Immunosorbent Assay, C-ELISA) is suitable for the determination of BTV in serum samples
Body detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this edition
file. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB 19489 laboratory biosafety common requirements
SN/T 1193 genetic testing laboratory technical requirements
3 clinical diagnosis
3.1 Epidemiology
3.1.1 Bluetongue disease is caused by the Bluetongue virus of the genus Reoviridae and is transmitted by bites from the civets to ruminants
The incidence of non-contact transmission of arbovirus disease.
3.1.2 BTV mainly causes morbidity and mortality in sheep, cattle and goats are often recessive infections, occasional onset and death.
3.1.3 The occurrence and distribution of blue tongue disease is closely related to the library library, mainly in the summer and autumn of the large number of activities in library library, especially in ponds, rivers
More common low-lying areas.
3.1.4 Susceptible animals have strong resistance to oral infections, the incidence of animal secretions and excretions within the virus content is very low, not
Cause the spread of blue tongue disease.
3.2 Clinical symptoms
3.2.1 incubation period of 3 days to 9 days. Disease early body temperature of sheep is 40.5 ℃ ~ 42 ℃, was reserved hot type, usually lasts 2 days ~ 3 days.
3.2.2 sick sheep lips edema and congestion, there salivation and runny nose and other phenomena. Oral congestion, was purple or blue-purple, soon oral mucosa
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