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GB/T 18634-2009: Determination of feed phytase activity -- Spectrophotometric method
Status: Valid GB/T 18634: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18634-2009 | English | 229 |
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Determination of feed phytase activity -- Spectrophotometric method
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GB/T 18634-2009
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| GB/T 18634-2002 | English | 319 |
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Determination of feed phytase activity -- Spectrophotometric method
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GB/T 18634-2002
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PDF similar to GB/T 18634-2009
Basic data | Standard ID | GB/T 18634-2009 (GB/T18634-2009) | | Description (Translated English) | Determination of feed phytase activity -- Spectrophotometric method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 10,163 | | Date of Issue | 2009-05-26 | | Date of Implementation | 2009-10-01 | | Older Standard (superseded by this standard) | GB/T 18634-2002 | | Quoted Standard | GB/T 14699.1 | | Regulation (derived from) | National Standard Approval Announcement 2009 No.7 (Total No.147) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the order spectrophotometry feeding phytase activity. This standard applies to the phytase product used as a feed additive, also applies to the added phytase feed. The method limit of quantification was 130 U/kg. | GB/T 18634-2009: Determination of feed phytase activity -- Spectrophotometric method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of feed phytase activity. Spectrophotometric method
ICS 65.120
B46
National Standards of People's Republic of China
Replacing GB/T 18634-2002
Determination of feed phytase activity
Posted 2009-05-26
2009-10-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard replaces GB/T 18634-2002 "Feeding phytase activity was measured by spectrophotometry."
This standard compared with GB/T 18634-2002 main changes are as follows.
--- Narrowed the scope of application of the method;
--- Change the minimum limit of quantification;
--- Changed the method of preparation of buffer solution;
--- Refine the determination of process steps;
--- Removed relatively law;
--- Expanded with the addition of phytase feed samples allowed two runs difference, relative deviation ≤15%.
Appendix A of this standard is an informative annex.
This standard by the National Standardization Technical Committee Feed Industry and focal points.
This standard is drafted by. Agricultural Quality Standards and Testing Technology Research Institute, Chinese Academy of Agricultural Sciences [National Feed Quality Supervision and Inspection
Heart (Beijing)], Guangdong overflow Dolly Biotechnology Co., Ltd., Wuhan Xinhua Yang Biotechnology Limited.
The main drafters of this standard. Ma Dongxia, Zhanzhi Chun, Shi Baojun, Suxiao Ou, Zhan Liansheng, Liangxue Xia, Zhang Su.
This standard replaces the standards previously issued as follows.
--- GB/T 18634-2002.
Determination of feed phytase activity
Spectrophotometry
1 Scope
This standard specifies the spectrophotometric determination of feed with phytase activity.
This standard applies to products phytase as a feed additive for use, but also to added phytase feed. Methods lowest
The limit of quantification of 130U/kg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 14699.1 feed samples
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
At a temperature of 37 ℃, under pH5.50 conditions, L solution per minute from sodium phytate concentration of 5.0mmol/release 1μmol inorganic phosphorus,
Is a unit of phytase activity, expressed in U.
Principle 4
Phytase at a certain temperature and pH conditions, the hydrolysis of the substrate phytate, and inositol phosphate derivatives being generated. In acidic solution,
Can form a yellow complex with ammonium molybdate vanadium, can be measured colorimetrically at 415nm wavelength.
5 Reagents and materials
Unless otherwise indicated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. Wash
Test vessels do not use phosphorus-containing cleaning agents.
5.1 potassium dihydrogen phosphate (KH2PO4). reference material.
5.2 acetate buffer (1), Ba (CH3COONa) = 0.25mol/L. Weigh 20.52g of anhydrous sodium acetate in 1000mL beaker, add
Into 900mL water and stir to dissolve, with glacial acetic acid pH adjusted to 5.50 ± 0.01, and transferred to a 1000mL volumetric flask and filled with distilled water
To volume. Stored at room temperature for 2 months effective.
5.3 acetate buffer (2), Ba (CH3COONa) = 0.25mol/L. Weigh 20.52g of anhydrous sodium acetate, 0.5g Triton X-100
(TritonX-100), 0.5g of bovine serum albumin (BSA) in 1000mL beaker, 900mL water and stir to dissolve, with glacial acetic acid tone
To pH 5.50 ± 0.01, and transferred to a 1000mL volumetric flask with distilled water to the mark. Store at room temperature for 2 months
effective.
5.4 substrate solution, Ba (C6H6O24P6Na12) = 7.5mmol/L. Weigh 0.69g sodium phytate (C6H6O24P6Na12, relative molecular mass
To 923.8, purity 95%), accurate to 0.1mg, placed in 100mL beaker, dissolved in about 80mL acetate buffer (5.2), with ice
Acetic acid to adjust the pH to 5.50 ± 0.01, transferred to a 100mL volumetric flask, and treated with acetate buffer (5.2) to the mark, using now
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