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GB/T 17999.8-2008: SPF chicken -- Microbiological surveillance -- Part 8: Examination of salmonella pullorum for SPF chicken
Status: Valid GB/T 17999.8: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 17999.8-2008 | English | 139 |
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SPF chicken -- Microbiological surveillance -- Part 8: Examination of salmonella pullorum for SPF chicken
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GB/T 17999.8-2008
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| GB/T 17999.8-1999 | English | 199 |
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SPF chicken--Tube agglutination test
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GB/T 17999.8-1999
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PDF similar to GB/T 17999.8-2008
Basic data | Standard ID | GB/T 17999.8-2008 (GB/T17999.8-2008) | | Description (Translated English) | SPF chicken -- Microbiological surveillance -- Part 8: Examination of salmonella pullorum for SPF chicken | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 6,655 | | Date of Issue | 2008-12-31 | | Date of Implementation | 2009-05-01 | | Older Standard (superseded by this standard) | GB/T 17999.7-1999 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.27 (Total No.140) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the technical requirements for Salmonella pullorum test. This section applies to the SPF chickens were separated and identified Salmonella pullorum. Serum plate agglutination test for Salmonella pullorum antibody test conducted, the results difficult to judge, can be carried out in this part of the re-examination. | GB/T 17999.8-2008: SPF chicken -- Microbiological surveillance -- Part 8: Examination of salmonella pullorum for SPF chicken
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
SPF chicken Microbiological surveillance Part 8. Examination of salmonella pullorum for SPF chicken
ICS 11.220
B41
National Standards of People's Republic of China
Replacing GB/T 17999.7-1999
SPF chickens microbiological monitoring
Part 8. SPF chicken Salmonella test
Posted 2008-12-31
2009-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts.
--- The first part 1. SPF chicken microbiological monitoring General;
Part --- Article 2. SPF chicken hemagglutination inhibition test;
Part --- Section 3. SPF chicken serum neutralization test;
Part --- Chapter 4. SPF chicken serum plate agglutination test;
Part --- Article 5. SPF chicken agar diffusion test;
--- Part 6. SPF chickens ELISA;
Part --- Article 7. SPF chicken embryo susceptibility testing;
Part --- Article 8. SPF chicken Salmonella testing;
--- Part 9. SPF chickens tube agglutination test;
Part --- Article 10. SPF chickens indirect immunofluorescence assay.
This section GB/T 17999 Part 8.
The partial revision of the reference to the NY/T 556-2002 "ILTV diagnostic technology" and OIE "terrestrial animals (mammals,
Birds and bees) Manual of diagnostic tests and vaccines, "the relevant provisions (fifth edition) of.
This Part replaces GB/T 17999.7-1999 "SPF chicken Salmonella test."
This section compared with the GB/T 17999.7-1999 The main changes are as follows.
--- An increase of live poultry cloacal swabs test sample;
--- Increased Salmonella bacterial antigen serological detection methods;
--- Added Appendix A "Gram staining and biochemical test result determination";
--- Test procedures were revised, bacterial examination procedures in advance;
--- In scope to further clarify the situation in this part;
--- Delete the reference standard;
--- Revised test procedures.
This section of Appendix A normative appendix.
This section proposed by the People's Republic of China Ministry of Agriculture.
This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized.
This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law
Adams Poultry Limited.
The main drafters of this section. song even East, Jiang Qian, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Liu Jiasen Secretary Chand, Guodong Chun, Yu Haibo,
Mengqing Wen.
This part of the standard replaces the previous editions are.
--- GB/T 17999.7-1999.
SPF chickens microbiological monitoring
Part 8. SPF chicken Salmonella test
1 Scope
This section GB/T 17999 specifies the technical requirements for Salmonella testing.
This section applies to SPF chickens were separated and identified Salmonella pullorum.
Serum plate agglutination test carried out to detect Salmonella antibody, the result is difficult to judge, it can be part of this re-examination.
Principle 2
Take sample to be assayed (intestinal contents, related to organ homogenates, live poultry cloacal swabs) was inoculated into enrichment medium enrichment is carried out, then the culture
Yang was transferred to the selection, identification of the culture medium, the selection of suspicious colonies were inoculated on nutrient agar, the culture was subjected to biochemical tests take
And serology tests to identify Salmonella.
3 Reagents and Equipment
3.1 Materials
3.1.1 Medium
Nutrient broth, DHL agar, the SS agar, bismuth sulfite agar (the BS), triple sugar iron medium (the TSI), nutrient agar, semisolid
Agar.
3.1.2 biochemical reagents
Sugar fermentation medium, peptone water, nitrate media, reagents oxidase, amino acid decarboxylase test medium, urea medium (Results
Determination see Appendix A).
3.1.3 Salmonella diagnostic sera
A-F O polyvalent serum, O9 serum factor, O12 serum factor, H-a serum factor, H-d serum factor, H-g. m factor and serum
H-g. p factor serum.
3.2 Equipment
37 ℃ incubator.
4 steps
4.1 Sampling
Aseptically ovary, liver, spleen and small intestine and cecum, live poultry cloacal swabs.
4.2 Isolation
The collected samples were placed in sterile homogenizer mortar or milled into a homogenized, add a small amount of nutrient broth dilution. Take culture or live poultry cloacal
Cavity swabs were on the SS or BS and DHL agar plate streaked, set (36 ± 1) ℃ cultured 24h ~ 48h. In DHL
If there is a medium brown transparent small colonies; or appear colorless translucent small circular colonies on agar plates SS; or BS agar
Board a black or dark green small colonies, was suspicious colonies. No If, after 24h ~ 48h culture of suspicious colonies, then take enrichment
Repeat streaking cultures incubated for 1 times.
4.3 Pathogen Identification
4.3.1 microscopy
Suspected colonies, smears for Gram stain, specific operations, see Appendix A. Examination shows Gram-negative bacilli, the size of
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