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GB/T 17999.7-2008 English PDF

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GB/T 17999.7-2008: SPF chicken -- Microbiological surveillance -- Part 7: Embryo susceptibility test for SPF chicken
Status: Valid

GB/T 17999.7: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB/T 17999.7-2008English139 Add to Cart 3 days [Need to translate] SPF chicken -- Microbiological surveillance -- Part 7: Embryo susceptibility test for SPF chicken Valid GB/T 17999.7-2008
GB/T 17999.7-1999English239 Add to Cart 2 days [Need to translate] SPF chicken--Examination of salmonella pullorum Obsolete GB/T 17999.7-1999

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Standard similar to GB/T 17999.7-2008

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Basic data

Standard ID GB/T 17999.7-2008 (GB/T17999.7-2008)
Description (Translated English) SPF chicken -- Microbiological surveillance -- Part 7: Embryo susceptibility test for SPF chicken
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 6,693
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Older Standard (superseded by this standard) GB/T 17999.6-1999
Quoted Standard GB/T 17999.1-2008
Regulation (derived from) National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the technical requirements of the embryo sensitive tests. This section applies to the SPF chickens to detect avian encephalomyelitis virus antibodies.

GB/T 17999.7-2008: SPF chicken -- Microbiological surveillance -- Part 7: Embryo susceptibility test for SPF chicken


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
SPF chicken Microbiological surveillance Part 7. Embryo susceptibility test for SPF chicken ICS 11.220 B41 National Standards of People's Republic of China Replacing GB/T 17999.6-1999 SPF chickens microbiological monitoring Part 7. SPF chicken embryo sensitivity test Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts. --- The first part 1. SPF chicken microbiological monitoring General; Part --- Article 2. SPF chicken hemagglutination inhibition test; Part --- Section 3. SPF chicken serum neutralization test; Part --- Chapter 4. SPF chicken serum plate agglutination test; Part --- Article 5. SPF chicken agar diffusion test; --- Part 6. SPF chickens ELISA; Part --- Article 7. SPF chicken embryo susceptibility testing; Part --- Article 8. SPF chicken Salmonella testing; --- Part 9. SPF chickens tube agglutination test; Part --- Article 10. SPF chickens indirect immunofluorescence assay. This section GB/T 17999 Part 7. This Part replaces GB/T 17999.6-1999 "SPF chicken embryo sensitivity test." This section compared with the GB/T 17999.6-1999 The main changes are as follows. --- Increase the informative A "yolk sac inoculation and EID50 determination" appendix; --- Re-enacted the establishment of experimental conditions and results criteria. Appendix A of this section is an informative annex. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized. This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law Adams Poultry Limited. The main drafters of this section. song even East Liujia Sen, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Jiang Qian, Secretary Chand, Yu Haibo, Mengqing Wen. This part of the standard replaces the previous editions are. --- GB/T 17999.6-1999. SPF chickens microbiological monitoring Part 7. SPF chicken embryo sensitivity test

1 Scope

This section GB/T 17999 specifies the technical requirements for susceptibility testing of embryos. This section applies to SPF chickens were detected avian encephalomyelitis virus antibodies.

2 Normative references

The following documents contain provisions which, through reference GB/T 17999 in this section constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB/T 17999.1-2008 SPF chickens microbiological monitoring - Part 1. SPF chicken microbiological monitoring General Principle 3 VanRoekel (CVCCAV35) strain is a strain of avian embryos adapt strains highly neurotropic. None of the anti Chicken flocks body pathogenic, causing muscular dystrophy, motor decreases, toes curling characteristic lesions. Immune or infected flocks After the chick embryo yolk sac inoculation of virus resistance.

4 Reagents and Equipment

4.1 Reagents 4.1.1 AE-VanRoekel virus (EID50 determination see Appendix A). 4.1.2 SPF eggs. 4.1.3 is (see sampling ratio GB/T 17999.1-2008) seized eggs. 4.2 Equipment 4.2.1 1mL syringe. 4.2.2 the 7th needle. 4.2.3 incubator. 4.2.4 clean bench. 4.2.5 Small incubator.

5 Procedure

5.1 Take the subject eggs, standard SPF eggs hatch to 6 days of age. 5.2 Take 6-day-old chick embryos were seized, each embryo by yolk sac inoculation (inoculation method see Appendix A) 0.2mL containing 100EID50 the AE-Van Roekel virus was inoculated in complete sealing wax, and set 6-day-old SPF chicken embryos inoculated with the virus and not the virus inoculation were used as a standard positive, Negative control. 5.3 inoculated chicken embryos, placed in 37 ℃ ~ 37.5 ℃ incubator hatching, once a day, according to the embryo, remove dead embryo, the first 12d after inoculation Remove all the chicken to be seized.

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